 | Bruce Greenwood als Bruce Wayne / Batman (voice) |
 | Jensen Ackles als Jason Todd / Red Hood (voice) |
 | Neil Patrick Harris als Dick Grayson / Nightwing (voice) |
 | Jason Isaacs als Ra's al Ghul (voice) |
 | John DiMaggio als Jack Napier / Joker / Red Hood (voice) |
 | Wade Williams als Black Mask (voice) |
 | Carlos Alazraqui als Chi Chi / Thug #1 / Baton (voice) |
 | Robert Clotworthy als Leon / Thug (voice) |
 | Gary Cole als Bobo / Commissioner Gordon / Shot / Guard (voice) |
 | Brian George als Ra's Assistant (voice) |
 | Kelly Hu als Ms. Li (voice) |
 | Phil LaMarr als Rick / Bulk (voice) |
 | Alexander Martella als Young Robin / Jason Todd (voice) |
 | Vincent Martella als Robin / Jason Todd (voice) |
 | Jim Piddock als Alfred Pennyworth (voice) |
 | Kevin Michael Richardson als Tyler Bramford (voice) |
 | Andrea Romano als Reporter #1 (voice) |
 | Dwight Schultz als Freddie / Drug Dealer (voice) |
 | Fred Tatasciore als Mercenary #1 / Amazo / Guard (voice) |
| Keri Tombazian als Reporter #2 / Fearsome Hand Leader (voice) |
 | Bruce Timm als The Riddler (voice) |
 | Michael Villani als Reporter #3 (voice) |
| Brandon Vietti | Director |
| Judd Winick | Writer |
| Judd Winick | Screenplay |
| Jay Oliva | Storyboard |
| Michael Uslan | Executive Producer |
| Margaret Hou | Editor |
| Bob Kane | Characters |
| Andrea Romano | Casting |
| Yan Jia | Digital Effects Supervisor |
| Benjamin Melniker | Executive Producer |
| Sam Register | Executive Producer |
| Gregory Noveck | Executive Producer |
| Alan Burnett | Co-Producer |
| Bobbie Page | Producer |
| Bruce Timm | Producer |
| Tim Yoon | Line Producer |
| Christopher Drake | Original Music Composer |
| George Brooks | Sound Effects Editor |
| Robert Hargreaves | Sound Effects Editor |
| Robert Hargreaves | Sound Designer |
