 | Troy Baker als Bruce Wayne / Batman / The Joker (voice) |
 | Eric Bauza als Leonardo (voice) |
 | Darren Criss als Raphael (voice) |
 | Kyle Mooney als Michelangelo (voice) |
 | Baron Vaughn als Donatello (voice) |
 | Rachel Bloom als Barbara Gordon / Batgirl (voice) |
 | Ben Giroux als Damian Wayne / Robin (voice) |
 | Brian George als Alfred Pennyworth (voice) |
 | Jim Meskimen als Comissioner Gordon / Jonathan Crane / Scarecrow (voice) |
 | Tom Kenny als Oswald Chesterfield Cobblepot / The Penguin (voice) |
 | John DiMaggio als Victor Fries / Mr. Freeze (voice) |
 | Tara Strong als Harleen F. Quinzel / Harley Quinn / Pamela Isley / Poison Ivy (voice) |
 | Carlos Alazraqui als Bane (voice) |
 | Cas Anvar als Ra's al Ghul (voice) |
 | Keith Ferguson als Harvey Dent / Two-Face / Baxter Stockman (voice) |
 | Andrew Kishino als Shredder (voice) |
| Mitchell Lestner | Sound Effects Editor |
| Jake Castorena | Director |
| Marly Halpern-Graser | Writer |
| Gene Vassilaros | Casting |
| Wes Gleason | Casting |
| Robert Ehrenreich | Editor |
| Kyle Stafford | Editor |
| Kevin Riepl | Music |
| Kimberly S. Moreau | Line Producer |
| Ben Jones | Producer |
| Sam Register | Executive Producer |
| Benjamin Melniker | Executive Producer |
| Michael Uslan | Executive Producer |
| Chris Viscardi | Executive Producer |
| Freddie Williams II | Comic Book |
| James Tynion IV | Comic Book |
| Bob Kane | Characters |
| Bill Finger | Characters |
| Kevin Eastman | Characters |
| Peter Laird | Characters |
| Paul Dini | Characters |
| Bruce Timm | Characters |
| Denny O'Neil | Characters |
| Neal Adams | Characters |
| Chuck Dixon | Characters |
| Doug Moench | Characters |
| Graham Nolan | Characters |
