 | Wesley Snipes als Eric Brooks / Blade |
 | Kris Kristofferson als Abraham Whistler |
 | Ron Perlman als Dieter Reinhardt |
 | Leonor Varela als Nyssa Damaskinos |
 | Norman Reedus als Scud |
 | Thomas Kretschmann als Eli Damaskinos |
 | Luke Goss als Jared Nomak |
 | Matt Schulze als Chupa |
 | Danny John-Jules als Asad |
 | Donnie Yen als Snowman |
 | Karel Roden als Karel Kounen |
 | Marit Velle Kile als Verlaine |
 | Tony Curran als Priest |
 | Daz Crawford als Lighthammer |
 | Santiago Segura als Rush |
 | Samuel Le als Jigsaw |
 | Marek Vašut als Golem |
| Pete Lee-Wilson als Blood Bank Doctor / Reaper |
 | Paul Kasey als Blood Bank Guard / Reaper |
 | Andrea Miltner als Blood Bank Nurse |
 | Ladislav Beran als Drug Dealer |
 | Jiří Maria Sieber als Blood Bank Guard |
| Bridge Markland als Vampire with Exposed Spine |
| Jamie Wilson als Reaper |
| Stuart Luis als Reaper |
| Ladislav Mohyla als Reaper |
| Jan Malík als Reaper |
 | Jan Révai als Reaper |
| Mário Wild als Reaper |
| Tomáš Böhm als Reaper |
| Zdenek Bubák als Reaper |
| Jan Loukota als Reaper |
| Jan Bursa als Reaper |
| Petr Krusalnicky als Reaper |
| Jaroslav Misek als Reaper |
| Karel Vávrovec als St. Cloud |
| Jaroslav Peterka als Choad |
 | Miloš Kulhavý als Little G |
| Ivan Mareš als Tea Bag |
 | Lennox Brown als Man in London Porno Shop |
| André Hyde-Braithwaite als Young Blade |
 | Pavel Cajzl als Moscow Vampire (uncredited) |
 | Olga Lounová als Prostitute (uncredited) |
 | Stephen R. Peluso als Paramedic (uncredited) |
 | John Salvitti als Moscow Vampire Attacking Blade (uncredited) |
 | Kenji Tanigaki als Moscow Vampire Attacking Blade (uncredited) |
| Michael Woods als Moscow Vampire Attacking Blade (uncredited) |
| Guillermo del Toro | Director |
| David S. Goyer | Writer |
| Wesley Snipes | Producer |
| Gabriel Beristain | Director of Photography |
| David S. Goyer | Executive Producer |
| Gene Colan | Characters |
| Avi Arad | Executive Producer |
| Toby Emmerich | Executive Producer |
| Nancy Foy | Casting |
| Patrick J. Palmer | Producer |
| Peter Amundson | Editor |
| Wendy Partridge | Costume Design |
| Marv Wolfman | Characters |
| Peter Frankfurt | Producer |
| Marco Beltrami | Original Music Composer |
| Lynn Harris | Executive Producer |
| Stan Lee | Executive Producer |
| Michael De Luca | Executive Producer |
| Jon Divens | Co-Producer |
| Andrea Clark | Casting Associate |
| Andrew J. Horne | Co-Producer |
| Peter Nicolakakos | Set Decoration |
| Wendy Cox | Production Supervisor |
| Scott Martin Gershin | Sound Designer |
| Michelle Taylor | Makeup Artist |
| Michael Madden | Set Designer |
| Gordon White | Set Designer |
| Bruce DiValerio | Construction Coordinator |
| Jeanette Freeman | Hairstylist |
| Michelle Weller | Production Supervisor |
| Mathew Waters | Supervising Sound Editor |
| John Ross | Sound Re-Recording Mixer |
| Jeffrey Kushon | Set Decoration |
| Phillip Tellez | Construction Coordinator |
| Constantine Sekeris | Conceptual Design |
| Jaromír Švarc | Art Direction |
| Martin Vačkář | Assistant Art Director |
| James F. Truesdale | Art Direction |
| Pavel Typolt | Production Manager |
| Milena Koubková | Set Designer |
| Carol Spier | Production Design |
| Dan Morski | Co-Art Director |
| Nikita Rae | Makeup Artist |
| Michael Keller | Sound Re-Recording Mixer |
| Mike Hyrman | Art Department Coordinator |
| Scott Martin Gershin | Supervising Sound Editor |
| Robert Duncan | Visual Effects Supervisor |
| Pavel Cajzl | Stunt Coordinator |
| Douglas Dresser | Location Manager |
| Anthony Gaudioz | Camera Operator |
| Jaromir Simek | Gaffer |
| Dominic Parker | Visual Effects Supervisor |
| Aaron Skalka | Transportation Coordinator |
| Marianna Krenova | Stand In |
| Blair Clark | Visual Effects Supervisor |
| Dirk Westervelt | Visual Effects Editor |
| Guy Hodgen | Additional Camera |
| Jon Sperry | Dialect Coach |
| Sarah Schubart | Visual Effects Editor |
| Nicholas Brooks | Visual Effects Supervisor |
| Susan MacLeod | Visual Effects Producer |
| Bill Abbott | Music Editor |
| Pavel Sterba | Location Manager |
| Terry Bradley | Visual Effects Supervisor |
| Craig Hayes | Visual Effects Supervisor |
| Ruth Greenberg | Visual Effects Producer |
| Klaus Fuxjäger | Camera Operator |
| Craig Fikse | Steadicam Operator |
| Peter S. Elliot | Visual Effects Editor |
| Barbora Kolarova | Special Effects Coordinator |
| H. Mark Vuille | Gaffer |
| Justin Martin | Visual Effects Supervisor |
| Moira Houlihan | Unit Publicist |
| Brian Richards | Music Editor |
| Vaclav Mottl | Transportation Coordinator |
| David Sosalla | Visual Effects Supervisor |
| Nordin Rahhali | Visual Effects Supervisor |
| George Macri | Visual Effects Producer |
| Viktor Lonek | Video Assist Operator |
| Bob Munroe | Visual Effects Supervisor |
| Edward Irastorza | Visual Effects Producer |
| Dominic Sidoli | Visual Effects Producer |
| Marese Langan | Key Hair Stylist |
| Elinor Rose Galbraith | Supervising Art Director |
| Michael Hertlein | ADR Editor |
| Alexandre Szabo-Fresnais | Camera Loader |
| Thomas Jones | ADR Supervisor |
| Jeff Ward | Martial Arts Choreographer |
| Christopher Sposa | Cableman |
| Chris Ferrence | Driver |
| Kelly Fraser | Assistant Costume Designer |
| Jayson Wall | Post Production Assistant |
| Sara Romilly | Post Production Supervisor |
| Freddy Bouciegues | Utility Stunts |
| Tim Larsen | Painter |
| James Weston | Armorer |
| Ladislav Hrubý | First Assistant Camera |
| Danny Saber | Additional Music |
| Ladislav Beran | Choreographer |
| Alex Matcham | Production Controller |
| Eva Dvorakova | Set Medic |
| Phred Lender | Systems Administrators & Support |
| Keith D. Fisher | Transportation Captain |
| John Weldy | Sculptor |
| Jan Kodera | Leadman |
| David Dupuis | Prosthetic Supervisor |
| Mark Fattibene | CG Supervisor |
| Wayne D. Barlowe | Creature Design |
| Jason Brubaker | Storyboard |
| Bryan Bowen | Sound Effects Editor |
| Petr Svoboda | Rigging Gaffer |
| Michal Houzvicka | Rigging Grip |
| Peter Eusebe | Boom Operator |
| Hector C. Gika | Sound Editor |
| David Rosenthal | I/O Supervisor |
| Mark Holding | Production Sound Mixer |
| Kandece Brown | Visual Effects Coordinator |
| Sissy Grover | Production Coordinator |
| John Kurlander | Scoring Mixer |
| Claudine Strasser | Script Supervisor |
| Robert Bernacchi | Associate Producer |
| Nick Allder | Special Effects Supervisor |
| Steve Watson | 24 Frame Playback |
| Laura Rindner | First Assistant Editor |
| Dan Muscarella | Color Timer |
| Ben Bowling | Electrician |
| Mike Macias | Lighting Technician |
| Jiri Tichacek | Production Accountant |
| Nerses Gezalyan | Sound mixer |
| Asregadoo Arundi | Digital Compositors |
| Vicki Wong | I/O Manager |
| Happy Walters | Music Supervisor |
| Robert Bernacchi | Unit Production Manager |
| Malcolm Sheehan | Key Grip |
| Miroslav Čáslavka | Set Dresser |
| John Emory | Grip |
| Stephani Hunter | Assistant Production Coordinator |
| David Dupuis | Key Makeup Artist |
| Philip Seeger | Third Assistant Director |
| Susan Cahill | Assistant Sound Editor |
| Craig Jurkiewicz | Foley Editor |
| Jerry Corda-Stanley | CG Animator |
| Clayton J. Barber | Fight Choreographer |
| Libor Hatas | Pilot |
| Gary Burritt | Negative Cutter |
| Minna Pyyhkala | Casting Assistant |
| Pete Anthony | Conductor |
| Heather Moore | Key Costumer |
| Tom Boyd | Musician |
| Warren J.W. Leathem | Lead Animator |
| Julian Wall | First Assistant Director |
| Jana Lynn Gold | Associate Editor |
| Clive Miles | Assistant Production Manager |
| Bill Daimant | Dolly Grip |
| Nigel Booth | Prosthetic Makeup Artist |
| Paul Anderson | 3D Artist |
| Matt Colleran | Sound Recordist |
| Jed M. Dodge | Dialogue Editor |
| Simon Werry | Aerial Camera |
| Greg Zimmerman | Foley Recordist |
| Gert Broekema | Property Master |
| Michael Hertlein | Dialogue Editor |
| Louis Creveling | Dialogue Editor |
| Michael Lindsay | Property Master |
| Robert Getty | Dialogue Editor |
| Mark Taylor | Second Assistant Director |
| TyRuben Ellingson | Concept Artist |
| Chas Bain | Focus Puller |
| Adam Glasman | Digital Colorist |
| Natasha Ivanova | Additional Dialogue |
| John Bannister | Key Scenic Artist |
| Jan Beran | Special Effects Technician |
| Jim Garrow | Colorist |
| Martina Götthansova | Second Second Assistant Director |
| Brian Basham | ADR Recordist |
| Nerses Gezalyan | Foley Mixer |
| Darrel Griffin | Data Wrangler |
| Leon Dudevoir | Production Executive |
| Michael K. O'Melia | Assistant Chief Lighting Technician |
| Jennifer Hunt Pedevilla | Payroll Accountant |
| Ray Scalice | General Manager |
| Betty Burkhart | Post Production Coordinator |
| Vera Trousilová | Assistant Accountant |
| Dave Jordan | Music Consultant |
| Rob Seager | First Assistant Accountant |
| Avtar Bains | Compositing Artist |
| Dan Feinstein | Rotoscoping Artist |
| Ron Bedrosian | ADR Mixer |
| Clay Dale | CG Artist |
| Lisa Bechard | Visual Effects Production Manager |
| Kenny Groom | Assistant Camera |
| Alexander Bscheidl | Second Assistant Camera |
| David Antol | Location Assistant |
| Alonzo Ruvalcaba | Head of Production |
| Tyler Ham | Production Assistant |
| Cammeron Truesdale | Production Runner |
| Nicola 'Nikki' Berwick | Stunt Double |
| Jim Ervin | Music Producer |
| Jemma Scott-Knox-Gore | Contact Lens Technician |
| Greg Runnels | Swing |
| Petr Brodsky | Extras Casting |
| Ian Unterreiner | Visual Effects Production Assistant |
| Leo Duranona | Storyboard Artist |
| Josh Campbell | Assistant Editor |
| Ray McIntyre Jr. | Visual Effects Supervisor |
| Donnie Yen | Martial Arts Choreographer |
| Gregory Palmer | Second Assistant Director |
| John T. Sweeney | Production Coordinator |
| Gary Archer | Prosthetics |
| Gary Powell | Stunts |
| Clay Cullen | Stunts |
| Lane Leavitt | Stunts |
