 | Barrie Ingham als Basil of Baker Street / Bartholomew (voice) |
 | Val Bettin als Dr. David Q. Dawson (voice) |
 | Vincent Price als Professor Ratigan (voice) |
 | Susanne Pollatschek als Olivia Flaversham (voice) |
 | Candy Candido als Fidget (voice) |
| Diana Chesney als Mrs. Judson (voice) |
 | Eve Brenner als The Mouse Queen (voice) |
 | Alan Young als Hiram Flaversham (voice) |
 | Basil Rathbone als Sherlock Holmes (voice) |
| Ellen Fitzhugh als Bar Maid (voice) |
| Walker Edmiston als Citizen (voice) |
 | Wayne Allwine als Thug Guard (voice) |
 | Tony Anselmo als Thug Guard (voice) |
 | Frank Welker als Toby the Dog / Felicia the Cat (voice) (uncredited) |
 | Laurie Main als Dr. Watson (archive sound) |
 | Shani Wallis als Lady Mouse (voice) |
| Melissa Manchester als Miss Kitty Mouse (voice) |
 | Charles Fleischer als Additional Voices (voice) (uncredited) |
| Burny Mattinson | Screenplay |
| Ed Austin | Director of Photography |
| David Michener | Director |
| Peter Young | Screenplay |
| Ron Clements | Screenplay |
| Burny Mattinson | Director |
| John Musker | Director |
| Burny Mattinson | Producer |
| Matthew O'Callaghan | Screenplay |
| James Melton | Editor |
| Mel Shaw | Screenplay |
| Ron Clements | Director |
| Roy M. Brewer Jr. | Editor |
| Paul Galdone | Novel |
| Eve Titus | Novel |
| David Michener | Screenplay |
| Bruce Morris | Screenplay |
| Henry Mancini | Original Music Composer |
| Steve Hulett | Screenplay |
| John Musker | Screenplay |
| Vance Gerry | Screenplay |
| Anthony DeRosa | Animation |
| Jennifer Yuan | Layout |
| Jay Jackson | Visual Effects |
| Ron Miller | Producer |
| Mark Henn | Supervising Animator |
| Glen Keane | Supervising Animator |
| Jack Wadsworth | Music Editor |
| Guy Vasilovich | Art Direction |
| Henry Mancini | Songs |
| Larry Grossman | Lyricist |
| Ellen Fitzhugh | Lyricist |
| Melissa Manchester | Songs |
| Melissa Manchester | Lyricist |
