 | Adewale Akinnuoye-Agbaje als Heavy Duty |
 | Christopher Eccleston als McCullen / Destro |
 | Lee Byung-hun als Storm Shadow |
 | Joseph Gordon-Levitt als The Doctor / Rex |
 | Sienna Miller als Ana Lewis / Baroness |
 | Rachel Nichols als Scarlet |
 | Ray Park als Snake Eyes |
 | Jonathan Pryce als U.S. President |
 | Saïd Taghmaoui als Breaker |
 | Channing Tatum als Duke |
 | Arnold Vosloo als Zartan |
 | Marlon Wayans als Ripcord |
 | Dennis Quaid als General Hawk |
 | Grégory Fitoussi als Baron de Cobray |
 | Leo Howard als Young Snake Eyes |
 | Karolína Kurková als Cover Girl |
 | David Murray als James McCullen - 1641 |
 | Kevin J. O'Connor als Doctor Mindbender |
 | Gerald Okamura als Hard Master |
 | Brandon Soo Hoo als Young Storm Shadow |
 | Christopher Akers als G.I. Joe Security Tech |
| Fabrice Baral als CNN Reporter |
 | Michael Benyaer als Flight Control Technician |
 | Peter Breitmayer als Dr. Hundtkinder |
 | Michael Broderick als Screaming Man |
 | Elena Evangelo als White House Staff |
| Mark Hames als White House Staff |
 | Jacques Frantz als Bastille Prison Warden |
 | Wayne Lopez als G.I. Joe Security Tech |
| Kellie Matteson als G.I. Joe Control Room Technician |
 | Burton Perez als Bravo Soldier |
 | Bob Rumnock als M.A.R.S. Lab Worker |
 | Robert Russell als Bastille Prison Priest |
| Ashley Sommers als Little Girl |
| Michael Sommers als Neo Viper Secret Service |
 | Gunner Wright als Secret Service Agent |
 | Duncan Bravo als Foreign General |
 | Charles Howerton als Foreign General |
 | Robert Almodovar als Foreign General |
| David Jean Thomas als Foreign General |
 | Ken Thomas als Apache Navigator |
| Frederic Doss als Apache Navigator |
| Buzz Covington als Apache Helicopter Pilot |
 | Ron Thompson als Apache Helicopter Pilot |
 | Brendan Fraser als Sgt. Stone (uncredited) |
 | Larry Hama als NATO General (uncredited) |
| Stephen Sommers | Director |
| Stuart Beattie | Screenplay |
| David Elliot | Screenplay |
| Paul Lovett | Screenplay |
| Lorenzo di Bonaventura | Producer |
| Bob Ducsay | Producer |
| Brian Goldner | Producer |
| Nancy Bishop | Casting |
| Ronna Kress | Casting |
| Ellen Mirojnick | Costume Design |
| Ed Verreaux | Production Design |
| Chad S. Frey | Art Direction |
| Jeremy Stewart | Animation |
| Dottie Starling | Visual Effects Supervisor |
| Mitchell Amundsen | Director of Photography |
| Alan Silvestri | Original Music Composer |
| Kevin Ishioka | Art Direction |
| Randy Moore | Art Direction |
| Brad Ricker | Art Direction |
| Anne Seibel | Art Direction |
| Greg Papalia | Supervising Art Director |
| Kate J. Sullivan | Set Decoration |
| Diana R. Lupo | Stunts |
| Stan Winston | Special Effects |
| Ronn Surels | Stunts |
| Rick James | Camera Trainee |
| Josh Tessier | Stunts |
| Shauna Duggins | Stunt Double |
| Bob Ducsay | Editor |
| Jim May | Editor |
| Boyd Shermis | Visual Effects Supervisor |
| JoAnn Perritano | Co-Producer |
| Greg Michael | Second Unit Director |
| Rhonda C. Gunner | Visual Effects Producer |
| Kelly Matsumoto | Editor |
| Danny Rafic | Visual Effects Editor |
| Per Hallberg | Supervising Sound Editor |
| Karen Baker Landers | Supervising Sound Editor |
| Daniel Sudick | Special Effects Supervisor |
| Amy Schmiederer | Key Makeup Artist |
| Peter Tothpal | Hair Department Head |
| Roxane Griffin | Key Hair Stylist |
| Gregory L. McMurry | Visual Effects Supervisor |
| Miranda Garrison | Choreographer |
| Tricia Miles | Post Production Supervisor |
| Kenneth Karman | Music Editor |
| Dan DiPrima | Music Editor |
| Shane Mahan | Effects Supervisor |
| Bryan Grill | Visual Effects Supervisor |
| David P.I. James | Visual Effects Producer |
| Bernd Angerer | Animation Supervisor |
| Darren Hendler | Digital Effects Supervisor |
| Greg Butler | Visual Effects Supervisor |
| Emma Phillips | Visual Effects Producer |
| Bryan Hirota | Visual Effects Supervisor |
| Chris Harvey | Visual Effects Supervisor |
| Chris Bond | Senior Visual Effects Supervisor |
| Sophia M. Crawford | Stunt Double |
| Jessie Graff | Stunt Double |
| Stuart Beattie | Story |
| Stephen Sommers | Story |
| Michael B. Gordon | Story |
| David Womark | Executive Producer |
| Stephen Sommers | Executive Producer |
| Gary Barber | Executive Producer |
| Roger Birnbaum | Executive Producer |
| Erik Howsam | Executive Producer |
| JoAnn Perritano | Unit Production Manager |
| Cliff Lanning | First Assistant Director |
| Ian Stone | Second Assistant Director |
| R.A. Rondell | Stunt Coordinator |
| Marcus Young | Fight Choreographer |
| Cliff Lanning | Associate Producer |
| Matthew Stuecken | Associate Producer |
| David Emmerichs | Camera Operator |
| David Emmerichs | Steadicam Operator |
| Christopher Duskin | Camera Operator |
| Brooks P. Guyer | Camera Operator |
| Sylvie Chesneau | Script Supervisor |
| Lee Orloff | Sound Mixer |
| Scott Millan | Sound Re-Recording Mixer |
| David Parker | Sound Re-Recording Mixer |
| Leslie Shatz | Sound Re-Recording Mixer |
| Bart Barbuscia | Set Dresser |
| Christopher Fuentes | Set Dresser |
| Tom Callinicos | On Set Dresser |
| Sara O'Donnell | Assistant Costume Designer |
| Carin Richardson | Assistant Costume Designer |
| Bob Morgan | Costume Supervisor |
| Amy Schmiederer | Makeup Artist |
| Cindy J. Williams | Makeup Department Head |
| Kentaro Yano | Makeup Artist |
| Kazu Hiro | Makeup Designer |
| Leo Corey Castellano | Makeup Designer |
| Sheryl Blum | Hairstylist |
| Matt Danon | Hairstylist |
| Fríða Aradóttir | Hairstylist |
| Eric Hugunin | Assistant Art Director |
| Kevin Loo | Assistant Art Director |
| Courtney Bright | Casting Associate |
| Tom Bellfort | Supervising ADR Editor |
| Chris Jargo | Supervising ADR Editor |
| Glenn T. Morgan | Foley Editor |
| Philip D. Morrill | Assistant Sound Editor |
| Tony Negrete | Assistant Sound Editor |
| Christopher Assells | Sound Effects Editor |
| Dino DiMuro | Sound Effects Editor |
| Dan Hegeman | Sound Effects Editor |
| Peter Staubli | Sound Effects Editor |
| Jon Title | Sound Effects Editor |
| David Werntz | Sound Effects Editor |
| Hector C. Gika | Sound Effects Editor |
| Benjamin Edelberg | Set Designer |
| Noelle King | Set Designer |
| Jeff Markwith | Set Designer |
| Patte Strong-Lord | Set Designer |
| Geoff Hubbard | Set Designer |
| James O.F. Hewitt | Set Designer |
| Joseph Hiura | Set Designer |
| Gary A. Lee | Set Designer |
| John Berger | Set Designer |
| Jonathan Taylor | Second Unit Director of Photography |
| Tad Griffith | Stunt Driver |
| Jeffrey J. Dashnaw | Stunts |
| Matt McColm | Stunt Driver |
| Clay Cullen | Stunts |
| Erik Rondell | Stunts |
| Eugene Collier | Stunt Double |
