 | Ben Stiller als Alex (voice) |
 | Chris Rock als Marty (voice) |
 | David Schwimmer als Melman (voice) |
 | Jada Pinkett Smith als Gloria (voice) |
 | Sacha Baron Cohen als Julien (voice) |
 | Cedric the Entertainer als Maurice (voice) |
 | Andy Richter als Mort (voice) |
 | Tom McGrath als Skipper / Fossa / Panicky Man on Subway (voice) |
 | Christopher Knights als Private (voice) |
 | Chris Miller als Kowalski (voice) |
 | Conrad Vernon als Mason (voice) |
 | Eric Darnell als Zoo Announcer / Lemur #1 / Fossa / Subway Car Announcer (voice) |
 | David Cowgill als Police Horse (voice) |
 | Elisa Gabrielli als Old Lady (voice) |
 | Stephen Apostolina als Police Officer (voice) |
 | Devika Parikh als News Reporter (voice) |
 | David P. Smith als Spider / Lemur #2 (voice) |
 | Cody Cameron als Willie (voice) |
 | Bob Saget als Zoo Animal (voice) |
 | Jeffrey Katzenberg als Rico (voice) (uncredited) |
| Mark Burton | Writer |
| Eric Darnell | Director |
| Billy Frolick | Writer |
| Tom McGrath | Director |
| James Baxter | Animation Supervisor |
| Trey Thomas | Animation Supervisor |
| Pilar Flynn | Production Supervisor |
| Paul Carrera | First Assistant Editor |
| Emilio Ghorayeb | Animation |
| Mark A. Hester | Editor |
| Teresa Cheng | Co-Producer |
| Tom McGrath | Writer |
| Clare Knight | Editor |
| Hans Zimmer | Original Music Composer |
| H. Lee Peterson | Editor |
| Mireille Soria | Producer |
| Eric Darnell | Writer |
| Paul Chung | Supervising Animator |
| Mark Binder | Sound Effects Editor |
| Tracey Dispensa | Animation Production Assistant |
| Terry Claborn | Color Timer |
| Melissa Muik | Music Editor |
| Richard L. Anderson | Supervising Sound Editor |
| Kory Heinzen | Production Illustrator |
| Erin Michael Rettig | Sound Engineer |
| Travis Koller | Visual Development |
| Edie Bielman | Additional Editing |
| Doug Pierce | Set Designer |
| Craig Heath | Sound Recordist |
| Christi Soper Hilt | Casting Associate |
| Damon Bard | Sculptor |
| Corban Gossett | Supervising Technical Director |
| Barbara Ford | Post-Production Manager |
| Robert C. Campion | Production Accountant |
| Mark Schoellkopf | Post Production Assistant |
| Greg Bulman | Systems Administrators & Support |
| Mark Coffey | Assistant Sound Editor |
| Philippe Gluckman | Visual Effects Supervisor |
| Dave Rosenbaum | Unit Manager |
| Leslee Feldman | Casting |
| Shannon Jeffries | Art Direction |
| Stewart Birnam | Software Engineer |
| José Antonio Macías | Translator |
| Gary L. Wohlleben | Production Controller |
| Lori Korngiebel | Post Production Supervisor |
| Jamie J. Silverman | Production Coordinator |
| Francois Antoine | Visual Effects |
| Kendal Cronkhite | Production Design |
| Ryeland Allison | Additional Music |
| Amy Christensen | Lighting Artist |
| Karey Kirkpatrick | Creative Consultant |
| Maryann Garger | Production Manager |
| Brian Brecht | Production Supervisor |
| Bruce Fowler | Orchestrator |
| Warren Zielinski | Musician |
| Jennifer Dahlman | Production Supervisor |
| Alessandro Ongaro | Visual Effects |
| Morten Folmer Nielsen | ADR Editor |
| Steve Mair | Musician |
| Ryan Harris | Production Supervisor |
| Betsy Nofsinger | Lighting Artist |
| Owen Slade | Musician |
| Jim Dooley | Additional Music |
| Heitor Pereira | Additional Music |
| Jonathan Williams | Musician |
| Andrew Findon | Musician |
| Lee Rasé | Production Coordinator |
| Tonia Pizzato | Production Supervisor |
| James Wood Wilson | Production Illustrator |
| Ted Tally | Creative Consultant |
| Bill Damaschke | Thanks |
| Olivier Mouroux | Publicist |
| Mark A. McGuire | Visual Effects Technical Director |
| Heitor Pereira | Musician |
| Paul Archibald | Musician |
| John Detke | Systems Administrators & Support |
| Jeff Lee | Systems Administrators & Support |
| Al Clay | Scoring Mixer |
| Donnachada Daly | Supervising Animator |
| Kelly Jean-Ware | Production Supervisor |
| Peter Mehlman | Creative Consultant |
| Andy Sisul | Assistant Sound Editor |
| Terri Anderson | Finance |
| Jeff Hayes | Character Modelling Supervisor |
| Anthony Pike | Musician |
| Mike Lovett | Musician |
| Fadi Basem Kandah | Production Supervisor |
| Ruben Hickman | Production Illustrator |
| Michael Min | Supervising Technical Director |
| Linda Bel | Animation |
| James Bolt | Sound Re-Recording Mixer |
| James Beshears | Executive In Charge Of Post Production |
| Buck Lewis | Character Designer |
| Erik Mattson | CG Supervisor |
| Dan Newell | Musician |
| Boguslav Kostecki | Musician |
| Alexandre Puvilland | Production Illustrator |
| Alan Meyerson | Additional Sound Re-Recording Mixer |
| Jaime Castañeda | Animation |
| Morgan R. Kelly | Animation |
| Wade Wilson | Sound Effects Editor |
| Nicholas Bucknall | Musician |
| Conrad Vernon | Creative Consultant |
| Debbie L. O'Keeffe | Thanks |
| Tom Holkenborg | Additional Music |
| Anna Noakes | Musician |
| Bruce White | Musician |
| Katherine Levin | Production Coordinator |
| Stacy Rentel | Production Supervisor |
| Yoriko Ito | Production Illustrator |
| Edwin R. Leonard | Thanks |
| Evan Smyth | Software Engineer |
| Shaun Collaco | Lighting Artist |
| Paul Clarvis | Musician |
| Jo Watanabe | Production Illustrator |
| Nicholas Long | Software Engineer |
| Cheryl Abood | Thanks |
| Robyn Rindge | Visual Effects Technical Director |
| James S. Levine | Additional Music |
| Andrew Crowley | Musician |
| Jonathan Evans-Jones | Musician |
| Mike Cutler | Systems Administrators & Support |
| Jill Hopper | Executive In Charge Of Production |
| Kristina Reed | Executive In Charge Of Production |
| Mark Berrow | Musician |
| Kristen D. Chidel | Executive In Charge Of Production |
| Rachel Bolt | Musician |
| Noel Wolfman | Production Supervisor |
| Ariandy Chandra | Title Designer |
| Rich Marco | Systems Administrators & Support |
| Craig Paulsen | First Assistant Editor |
| Matthieu Grospiron | Lighting Artist |
| Jennifer Freeman | Production Supervisor |
| Claudia Southmartin | Production Supervisor |
| Nick Ingman | Conductor |
| Bill Diaz | Animation |
| Jakob Hjort Jensen | Animation |
| Van Phan | Animation |
| Cristian Dumitriu | Modeling |
| Dug Stanat | Visual Effects |
| John O'Sullivan | Systems Administrators & Support |
| Holly Edwards | Production Supervisor |
| Gavin Greenaway | Conductor |
| Julia Woolf | Visual Development |
| Serguei Kouchnerov | Animation |
| Kevin Vassey | Visual Effects |
| Alison Fedrick | Production Coordinator |
| Philip R. Garrett | Production Supervisor |
| Raffaella Filipponi | Animation |
| Antony Gray | Animation |
| James Hull | Animation |
| Heather Knight | Animation |
| Andre LeBlanc | Visual Effects |
| Bart Feliciano | Systems Administrators & Support |
| Derek Mann | Systems Administrators & Support |
| Stephen E. Ross | Systems Administrators & Support |
| Tim Toll | Systems Administrators & Support |
| Jessie Carbonaro | Production Coordinator |
| Trinity Murphy | Production Coordinator |
| Andre de Oliviera Araujo | Production Supervisor |
| Andy Nelson | Sound Re-Recording Mixer |
| Jeremy Bernstein | Animation |
| Chung Chan | Animation |
| Nick Craven | Animation |
| Ethan Hurd | Animation |
| Sean Mahoney | Animation |
| David Fent | Systems Administrators & Support |
| Mo Henry | Negative Cutter |
| Cindy Hong | Lighting Artist |
| Anna Behlmer | Sound Re-Recording Mixer |
| Roberto Espanto Domingo | Animation |
| Jeffrey Joe | Animation |
| Bill E. Miller | Animation |
| William Opdyke | Visual Effects |
| Scott Kilty | Systems Administrators & Support |
| Joe Campana | Associate Editor |
| Robyn Mesher | Production Coordinator |
| Kevan Shorey | Animation |
| Scott Wright | Animation |
| Philippe Tilikete | Character Designer |
| Andrew Wheeler | Visual Effects |
| Ellen Poon | VFX Artist |
| Michael McNeill | Lighting Artist |
| Reuben Simon | Sound Effects Editor |
| Cassidy Curtis | Animation |
| Lou Dellarosa | Animation |
| Eric Deuel | Animation |
| Ben Rush | Animation |
| Charlie Ramos | Modeling |
| Jake Walker | Musician |
| Dave Burgess | Animation |
| Melanie Cordan | Animation |
| Kevin MacLean | Animation |
| Trish Schutz-Krause | Animation |
| Craig Kellman | Lead Character Designer |
| Joshua West | Modeling |
| Scott Liedtka | Visual Effects |
| Joe Monteleone | Assistant Editor |
| Jenny Wolfe-Binder | Production Coordinator |
| Dan O'Connell | Foley |
| Rick Giovinazzo | Orchestrator |
| David A. Whittaker | Sound Effects Editor |
| Arnaud Berthier | Animation |
| Kenny Chung | Animation |
| Kevin Koch | Animation |
| Fredrik Nilsson | Animation |
| Nick Foster | Visual Effects |
| Kerry Shea | Production Manager |
| Walt Fowler | Orchestrator |
| Luci Napier | Animation |
| Jason Schleifer | Animation |
| Nico Marlet | Character Designer |
| Robert Lurye | Lighting Artist |
| Tony Cosanella | Production Supervisor |
| Patrick Bonneau | Animation |
| Laurent Kermel | Visual Effects |
| Angela Ensele | Production Coordinator |
| Pres Romanillos | Animation |
| Theodore Anthony Lee Ty | Animation |
| Chris Wahl | Animation |
| Paul Rademacher | Visual Effects |
| Daniel Rich | Systems Administrators & Support |
| Hannibal P. Odisho | Production Coordinator |
| Jenna Grigg Thomas | Production Supervisor |
| Mariko Hoshi | Animation |
| Carlos M. Rosas | Animation |
| David Spivack | Animation |
| Karen Smith | VFX Artist |
| Frank Sabia Jr. | Lighting Artist |
| Stephanie C. Lee | Production Coordinator |
| Ray Felipe | Script Researcher |
| Robert Renga | Sound Recordist |
| Manu Ittina | Animation |
| Marek Kochout | Animation |
| David Caeiro Cebrián | Visual Effects |
| Christopher Knights | First Assistant Editor |
| Celeste Leger | Casting Assistant |
| Steve Mason | Animation |
| John K. Dodelson | Modeling |
| Thomas Jones | Supervising ADR Editor |
| Wayne Hellinger | Post Production Coordinator |
| Ewan Johnson | Head of Layout |
| Jim Mainard | Head of Research |
| York N. Schueller | Animation Technical Director |
| Noah Klocek | Matte Painter |
| James Ashwill | Foley Mixer |
| Timothy Peeler | Digital Colorist |
| Kristen D. Chidel | Production Executive |
| Jane Hartwell | Head of Production |
| Julie Imboden Keel | Music Coordinator |
| Linda Bork | Production Assistant |
| Chris Douridas | Music Consultant |
| John T. Cucci | Foley Artist |
| Dawn Turner | Visual Effects Production Assistant |
| Walt Dohrn | Story Artist |
| Troy Porter | ADR Mixer |
| Laverne Dewberry | ADR Recordist |
| Eric Lessard | Senior Animator |
