 | Ben Stiller als Greg Focker |
 | Robert De Niro als Jack Byrnes |
 | Dustin Hoffman als Bernie Focker |
 | Barbra Streisand als Rozalin Focker |
 | Blythe Danner als Dina Byrnes |
 | Teri Polo als Pam Byrnes |
 | Owen Wilson als Kevin Rawley |
 | Spencer Pickren als Little Jack 'L.J.' Byrnes |
| Bradley Pickren als Little Jack 'L.J.' Byrnes |
 | Alanna Ubach als Isabel Villalobos |
 | Ray Santiago als Jorge Villalobos |
 | Tim Blake Nelson als Officer LeFlore |
 | Shelley Berman als Judge Ira |
 | Kali Rocha als Flight Attendant |
 | Dorie Barton als Airline Clerk |
 | Jack Plotnick als Rent-a-Car Agent |
| Wayne Thomas Yorke als Airport Security Guard |
| B.J. Hansen als B.J. |
| J.P. Manoux als Local Cop |
 | Myra Turley als Admitting Nurse |
 | Vahe Bejan als Gunther - Immigrant Man |
 | Kathleen Gati als Venka - Immigrant Woman |
 | Angelo Tiffe als Businessman |
| Kyle T. McNamee als Undercover Waiter |
 | Cedric Yarbrough als Prison Guard |
 | Max Hoffman als Woody Focker |
| Benjamin Trueblood als Newborn Baby |
 | Roberto Garcia als Waiter |
| Bruno Coon als Strolling Minister / Band Member |
| Rock Deadrick als Wedding Band |
| David Sutton als Wedding Band |
 | Bernadette Pérez als Girl Bus Driver |
 | Linda O'Neil als Girl on Bus |
 | Tiffany Turner als Girl on Bus |
 | David Zimmerman als Dom Focker (uncredited) |
| Lenny Vullo | Unit Production Manager |
| Lee Haxall | Editor |
| Amy Sayres | Executive Producer |
| Jane Rosenthal | Producer |
| Alan Baumgarten | Editor |
| Jim Herzfeld | Screenplay |
| Nancy Tenenbaum | Executive Producer |
| Jon Poll | Editor |
| Sara Andrews | Set Decoration |
| Francine Maisler | Casting |
| Carol Ramsey | Costume Design |
| John Schwartzman | Director of Photography |
| Randy Newman | Original Music Composer |
| Rusty Smith | Production Design |
| Jay Roach | Producer |
| Andrew Neskoromny | Art Direction |
| Jay Roach | Director |
| Marc Hyman | Screenplay |
| Robert De Niro | Producer |
| Jon Poll | Co-Producer |
| Greg Glienna | Characters |
| Mary Ruth Clarke | Characters |
| Larry Stuckey | Associate Producer |
| John Hamburg | Screenplay |
| Orlando Avila | Driver |
| John A. Brubaker | Transportation Coordinator |
| Renzo Bartolotta | Electrician |
| Craig Alpert | Additional Editing |
| Melissa Bartley | Art Department Assistant |
| Clyde E. Bryan | First Assistant Camera |
| Fran Allgood | Set Costumer |
| Adam Braid | Set Dressing Artist |
| Bill Meadows | Sound Recordist |
| Cherie Tamai | First Assistant Sound Editor |
| Jonathan Sacks | Orchestrator |
| David D. Johnson | Visual Effects Supervisor |
| Teressa Hill | Key Hair Stylist |
| Christopher Woodworth | Scenic Artist |
| Peter Oillataguerre | Executive In Charge Of Production |
| Reese St. Amant | Painter |
| Bruno Coon | Additional Music |
| Brigette Lester | Production Coordinator |
| Ken McLaughlin | Production Sound Mixer |
| Lois DeArmond | Assistant Costume Designer |
| Ross Gallo | Stunts |
| Samuel R. Harrison III | Video Assist Operator |
| Michael Ambrose | Gaffer |
| Michael Morgenthal | Production Accountant |
| Clay Pinney | Special Effects Supervisor |
| Jonah Markowitz | Assistant Art Director |
| Will Thayer | Carpenter |
| Ted Yonenaka | Craft Service |
| Dennis McCarthy | Picture Car Coordinator |
| Ahmadu Garba | Post Production Assistant |
| John Polce | Stand In |
| Jimmy Culligan | Rigging Grip |
| Ryan Claridge | Greensman |
| Carmine Goglia | Standby Painter |
| Marisa Aboitiz | Costume Supervisor |
| Melanie Banders | Loader |
| Jill Frank | Assistant Director |
| Frank Dorowsky | Rigging Gaffer |
| Louise Hone | Foley |
| John Naehrlich | Leadman |
| Ian Fox | Camera Operator |
| Kate Biscoe | Makeup Department Head |
| Andy Clement | Animatronic and Prosthetic Effects |
| Frank D. Menges | Sound Mixer |
| Kevin Cross | Set Designer |
| Victoria Wood | Wigmaker |
| Eugene McCarthy | Property Master |
| Rhonda Sherman | Studio Teachers |
| Douglas Womack | Construction Coordinator |
| Wilma Garscadden-Gahret | Script Supervisor |
| Craig Scagnetti | Lighting Technician |
| Ryan Ferro | Boom Operator |
| Tracy Bennett | Still Photographer |
| Carol Pershing | Hairstylist |
| Steve D. Weinmuller | Transportation Captain |
| Scott C. Keys | Best Boy Electric |
| Bryan Corbett | Construction Foreman |
| Kurt E. Soderling | Aerial Director of Photography |
| George H. Anderson | Supervising Sound Editor |
| Jennifer R. Blair | Art Department Coordinator |
| Les T. Tomita | Key Grip |
| Lisa Rodgers | Post Production Supervisor |
| Bobby Mara | Propmaker |
| Doug Coleman | Stunt Coordinator |
| Pamela Lynn Thomas | Casting Associate |
| Jason W. Jennings | Sound Effects Editor |
| Terry Baliel | Hair Department Head |
| David DeLeon | Makeup Artist |
| Grady Holder | Makeup Effects |
| Andrew Lipschultz | Unit Publicist |
| Randall D. Wilkins | Set Designer |
| Guy Hietala | Set Painter |
| Cass Asher | Stunt Double |
| Gary Burritt | Negative Cutter |
| Doreen Austria | Graphic Designer |
| Matthew Haskins | Additional Second Assistant Camera |
| Nicki Johnson | Assistant Production Coordinator |
| Gail Rose | First Assistant Accountant |
| Marlene Marks | Payroll Accountant |
| Doug DuRose | Assistant Property Master |
| Vanessa Rodriguez Spencer | Casting Assistant |
| Judith A. Cory | Hairdresser |
| Gabriel Hardman | Storyboard Artist |
| Renée Tondelli | Supervising ADR Editor |
| Donald Krause | Special Effects Technician |
| Rebecca Feldman | Second Assistant Accountant |
| Doug Coleman | Second Unit Director |
| Rick Granville | Production Secretary |
| Ray Neapolitan | Assistant Editor |
| Rick Harris | Key Rigging Grip |
| Denise Anderson Poore | Key Set Production Assistant |
| Christine Wada | Key Costumer |
| Douglas J. Stewart | Textile Artist |
| Thomas DeRose | Assistant Chief Lighting Technician |
| Leslie A. Tokunaga | Construction Buyer |
| Derek Casari | ADR Engineer |
| Kurt E. Soderling | Visual Effects Camera |
| Goro Koyama | Foley Artist |
| John Johnson | Additional Still Photographer |
| Kevin Lane Hummel | Assistant Location Manager |
| Maryann Marchetti | Assistant Makeup Artist |
| David Lucarelli | ADR Recordist |
| Marisa Ferrey | Second Second Assistant Director |
| Calvin Callaway | Production Assistant |
| Charleen Richards-Steeves | ADR Mixer |
| Will Dearborn | Second Assistant Camera |
| Anthea Strangis | Assistant Accountant |
| Richard Lilly | Location Assistant |
| Stephen Richards | Additional Production Assistant |
| James Matheny | Dialogue Editor |
| Rick Avery | Stunt Double |
| Kurt Smith | Color Timer |
