 | Chris Hemsworth als Henry / Agent H |
 | Tessa Thompson als Molly Wright / Agent M |
 | Rebecca Ferguson als Riza Stavros |
 | Kumail Nanjiani als Pawny (voice) |
 | Rafe Spall als Agent C |
 | Laurent Bourgeois als Alien Twin #1 |
 | Larry Bourgeois als Alien Twin #2 |
 | Emma Thompson als Agent O |
 | Liam Neeson als Agent High T |
 | Mandeiya Flory als Young Molly |
 | Kayvan Novak als Vungus / Nasr / Bassam |
 | Annie Burkin als Nerlene |
 | Tim Blaney als Frank the Pug (voice) |
 | Spencer Wilding als Luca Brasi |
 | Marcy Harriell als Molly's Mom |
 | Inny Clemons als Molly's Dad |
| Thom Fountain als Worm Guy #1 (voice) |
 | Drew Massey als Worm Guy #2 (voice) |
 | Aaron Serotsky als Man in Black #1 |
 | Matthew Bates als FBI HR Guy |
| Sartaj Garewal als CIA Recruiter |
 | Stephen Wight als Guy |
 | Brian Silliman als Cabbie |
| John Sousa als Jimmy the Alien |
 | Jeff Kim als Lead MIB Agent |
 | Michael Adams als MIB Lead Interrogator |
 | Andrew Greenough als Freddie the Alien |
 | Natasha Culzac als Nightclub Waitress |
| David Hartley als MIB Forensics Agent |
 | Paul Brennen als Alien Boss |
 | Ania Sowinski als Alien Woman / Boss' Wife |
 | Stefan Kalipha als Antique Shopkeeper |
 | Daniel Scott-Smith als Nightclub Bartender |
 | Vincent Pirillo als MIB Security Guard |
 | Kaya Brown-Hallam als NIghtclub Patron #1 |
 | Beau Fowler als Nightclub Patron #2 |
 | Lukas DiSparrow als Nightclub Patron #3 |
 | Andy Beckwith als Viper Room Player |
 | Romanos Blanco als Man at Eiffel Tower |
 | Aistė Diržiūtė als Woman at Eiffel Tower |
 | Anatole Taubman als French Salesman |
 | Sorel Johnson als Nervous Forensic Tech |
 | Paul Smith als Proprietor (Charlie) |
 | Mike Capozzola als Veteran MIB Agent |
 | J.J. Abrams als Alien on TV Monitor (uncredited) |
 | Elle Black als Alien Dancer (uncredited) |
 | Donald Glover als Alien on TV Monitor (uncredited) |
 | Ariana Grande als Alien on TV Monitor (uncredited) |
 | Elon Musk als Alien on TV Monitor (uncredited) |
 | Hiten Patel als Tech Geek (uncredited) |
 | Dimitri Vegas als Undercover Alien (uncredited) |
 | Mike Lewis |
 | Luke Johnson als Dancer (uncredited) |
| F. Gary Gray | Director |
| Lowell Cunningham | Characters |
| Walter F. Parkes | Producer |
| Laurie MacDonald | Producer |
| Steven Spielberg | Executive Producer |
| Barry Sonnenfeld | Executive Producer |
| E. Bennett Walsh | Executive Producer |
| Christian Wagner | Editor |
| Zene Baker | Editor |
| Stuart Dryburgh | Director of Photography |
| Charles Wood | Production Design |
| Matt Willard | Editor |
| Danny Elfman | Original Music Composer |
| Chris Bacon | Original Music Composer |
| Art Marcum | Screenplay |
| Matt Holloway | Screenplay |
| Lowell Cunningham | Comic Book |
| Edward Cheng | Executive Producer |
| Howard Chen | Executive Producer |
| Riyoko Tanaka | Executive Producer |
| David Beaubaire | Executive Producer |
| David Rubin | Casting |
| Reg Poerscout-Edgerton | Casting |
| Penny Rose | Costume Designer |
| Jerome Chen | Visual Effects Supervisor |
| Daniel Kramer | Visual Effects Supervisor |
| Wade Eastwood | Stunt Coordinator |
| Wade Eastwood | Second Unit Director |
| Michael Sharp | Unit Production Manager |
| E. Bennett Walsh | Unit Production Manager |
| Lisa C. Satriano | First Assistant Director |
| David Keadell | Second Assistant Director |
| Deven LeTendre | Co-Producer |
| Michael Sharp | Co-Producer |
| Tom Brown | Supervising Art Director |
| John Bush | Set Decoration |
| Jeremy Woodhead | Prosthetic Designer |
| Peter Lindsay | Sound Designer |
| Paul Corbould | Special Effects Supervisor |
| Gemma Kingsley | Graphic Designer |
| Giles Keyte | Still Photographer |
| Lukasz Jogalla | Second Unit Director of Photography |
| Christian Joyce | Sound Mixer |
| Julian Townsend | Sound Mixer |
| Julian Slater | Sound Re-Recording Mixer |
| Hamilton Sterling | Sound Designer |
| Lennart Breede | Visual Effects Technical Director |
| Tim McGovern | Digital Effects Supervisor |
| Buddie Wilkinson | Standby Property Master |
| Chris Waegner | Visual Effects Supervisor |
| Joe Cash | Props |
| Wolfgang Stegemann | Fight Choreographer |
| Bobby Holland Hanton | Stunt Double |
| Tara Macken | Stunt Double |
| James Apps | Stunts |
| George Bailey | Stunts |
| Oliver Bailey | Stunts |
| Jason Beeston | Stunts |
| Mauro Calo | Stunts |
| Kieran Clarke | Stunts |
| Lucy Cork | Stunts |
| Kachina Dechert | Stunts |
| Craig Dolby | Stunts |
| Rick English | Stunts |
| Niki Faulkner | Stunts |
| Stephane Fiossanangaye | Stunts |
| David Forman | Stunts |
| Sofian Francis | Stunts |
| Richard Hall | Stunts |
| Matt Hermiston | Stunts |
| Timo Honsa | Stunts |
| Erol Ismail | Stunts |
| Tomasz Krzemieniecki | Stunts |
| Adrian McGaw | Stunts |
| Cali Nelle | Stunts |
| Andy Pilgrim | Stunts |
| Laurent Plancel | Stunts |
| Juliet Reeve | Stunts |
| Nik Schodel | Stunts |
| Tony van Silva | Stunts |
| Jeremie Vigot | Stunts |
| Kaloian Vodenicharov | Stunts |
| Ben Wilkinson | Property Master |
| Lisa Vick | Script Supervisor |
| Chris Plevin | "A" Camera Operator |
| Ashley Bond | First Assistant "A" Camera |
| Nick Thursby | Second Assistant "A" Camera |
| Vince McGahon | "B" Camera Operator |
| Vince McGahon | Steadicam Operator |
| Lewis Hume | First Assistant "B" Camera |
| Archie Muller | Second Assistant "B" Camera |
| Peter Welch | Digital Imaging Technician |
| Hannah Walter | Costume Supervisor |
| Lucy Bowring | Assistant Costume Designer |
| Sophie Hart-Walsh | Assistant Costume Designer |
| Jason Marshall | Key Costumer |
| Anna Spencer | Key Costumer |
| Holly Rosenthal | Costumer |
| Lizzie Moul | Costumer |
| Elena Taborra | Costumer |
| Amy Pollitt | Costumer |
| Martin Clarke | Costumer |
| Hannah Warren | Costumer |
| Dan Blacklock | Costumer |
| Jeremy Woodhead | Makeup Designer |
| Jeremy Woodhead | Hair Designer |
| Robb Crafer | Makeup & Hair |
| Robb Crafer | Prosthetic Makeup Artist |
| Belinda Hodgson | Makeup Artist |
| Phyllis Temple | Makeup Artist |
| Matteo Silvi | Makeup Artist |
| Tilly Calder | Makeup & Hair |
| Luca Vannella | Hairstylist |
| Lacey Redway | Hair Designer |
| Tracey Smith | Hairstylist |
| Noriko Watanabe | Makeup & Hair |
| Chuck Finch | Gaffer |
| Richard Merrell | Best Boy Electric |
| Steve Wood | Best Boy Electric |
| Benjamin Caldwell | Electrician |
| Perry Cullen | Electrician |
| Timothy Neill | Electrician |
| Jack Ridout | Electrician |
| Tommy Finch | Rigging Gaffer |
| Steve Ellingworth | Key Grip |
| Dave Wells | Best Boy Grip |
| Charlie Wall | Dolly Grip |
| Ken Musson | Dolly Grip |
| Peter Hayley-Barker | Dolly Grip |
| Peter Lindsay | Sound Mixer |
| Kate Morath | Boom Operator |
| Jamie Lengyel | Location Manager |
| Stephen Parker | Location Manager |
| Lindsey Powell | Location Manager |
| Matt Craufurd | Assistant Location Manager |
| Dan Peacock | Assistant Location Manager |
| Matt Risebrow | Assistant Location Manager |
| Pamela Joyce | Production Supervisor |
| Emma Hazell | Production Coordinator |
| Craig Jamieson | Assistant Production Coordinator |
| Felicity Thorpe | Travel Coordinator |
| Alistair Steele | Production Secretary |
| Josh Allan | Production Accountant |
| Mike DeSouza | Assistant Accountant |
| Katherine Holder | Payroll Accountant |
| Georgina Voysey | Payroll Accountant |
| Alana Self | Payroll Accountant |
| Tom Rothwell | Assistant Accountant |
| Khoa Dong | Assistant Accountant |
| Grace Heath | Assistant Accountant |
| Harry Skinner | Assistant Accountant |
| Tom Ray | Assistant Accountant |
| Jack Skinner | Assistant Accountant |
| Paul J. Hayes | Construction Manager |
| Amanda Pettett | Construction Coordinator |
| Nicola Dewey | Construction Coordinator |
| Julian Ashby | Art Direction |
| David Doran | Art Direction |
| Chris 'Flimsy' Howes | Art Direction |
| Mark Swain | Art Direction |
| Sue Whitaker | Art Direction |
| Sarah Bicknell | Assistant Art Director |
| Alex Bowens | Assistant Art Director |
| James M. Spencer | Assistant Art Director |
| Richard Hardy | Assistant Art Director |
| Choi Ho Man | Assistant Art Director |
| Alison Clements | Draughtsman |
| Diego De Lajonquiere | Draughtsman |
| Alfredo Lupo | Draughtsman |
| Albert McCausland | Draughtsman |
| Andy Young | Draughtsman |
| Paul Catling | Concept Artist |
| Tim Hill | Concept Artist |
| Pete Thompson | Concept Artist |
| Norman Walshe | Concept Artist |
| Ivan Weightman | Concept Artist |
| John Colebourn | Storyboard Artist |
| Ferenc Nothóf | Storyboard Artist |
| Kathryn Prince | Sculptor |
| Sarah Iovino | Art Department Coordinator |
| Huw Arthur | Standby Art Director |
| Tonja Schürmann | Assistant Set Decoration |
| Clare Holton | Set Decorating Coordinator |
| Matt Cooke | Assistant Property Master |
| Katherine McCormack | Unit Publicist |
| Mark Johnstone | Third Assistant Director |
| Brittany Smith | Third Assistant Director |
| Marlon Beyer Rieger | Third Assistant Director |
| Jamie Graham | Third Assistant Director |
| Barney Shakespeare | Third Assistant Director |
| Lillie Jeffrey | Casting Associate |
| Tamsyn Manson | Casting Assistant |
| Rory Okey | Casting Assistant |
| Andrea Bunker | Casting Associate |
| Matt Lander | Casting Assistant |
| Kirsty Savory | Craft Service |
| Andrew Jack | Dialect Coach |
| Jeremy Braben | Aerial Director of Photography |
| Bryan Baverstock | Transportation Captain |
| Laura Dalton | Transportation Coordinator |
| Richard Conkling | Additional Editor |
| Michael A. Webber | Additional Editor |
| Jonathan Silver | Additional Editor |
| Scott A. Jacobs | Assistant Editor |
| Isabel Zippert | Assistant Editor |
| Matthew Ozerski | Assistant Editor |
| Andrew McGivney | Assistant Editor |
| Hillary Hendler | Post Production Coordinator |
| Brooke Lowery | Post Production Coordinator |
| Paul N.J. Ottosson | Sound Re-Recording Mixer |
| Paul N.J. Ottosson | Sound Designer |
| Paul N.J. Ottosson | Supervising Sound Editor |
| Greg ten Bosch | Sound Effects Editor |
| Bobbi Banks | Supervising ADR Editor |
| Daniel Saxlid | ADR Editor |
| Ryan Juggler | ADR Editor |
| Robert Troy | Dialogue Editor |
| Mark Pappas | Foley Supervisor |
| Gary A. Hecker | Foley Artist |
| Rick Owens | Foley Artist |
| Kyle Rochlin | Foley Mixer |
| Howard London | ADR Mixer |
| Bill Abbott | Music Editor |
| Robb Boyd | Music Editor |
| Steve Bartek | Orchestrator |
| David Slonaker | Orchestrator |
| Edward Trybek | Orchestrator |
| Pete Anthony | Conductor |
| Melisa McGregor | Music Coordinator |
| Clay Cullen | Stunts |
| Clara Martinez | Visual Effects Coordinator |
