 | James Corden als Peter Rabbit (voice) |
 | Rose Byrne als Beatrix 'Bea' Potter / Jemima Puddle-Duck (voice) |
 | Domhnall Gleeson als Thomas McGregor / Mr. Jeremy Fisher (voice) |
 | Margot Robbie als Flopsy Rabbit / Narrator (voice) |
 | Elizabeth Debicki als Mopsy Rabbit (voice) |
 | Daisy Ridley als Cottontail Rabbit (voice) |
 | Colin Moody als Benjamin Bunny (voice) |
 | Sam Neill als Mr. McGregor / Tommy Brock (voice) |
 | Marianne Jean-Baptiste als Harrods General Manager |
 | Felix Williamson als Derek |
 | Sia als Mrs. Tiggy-Winkle (voice) |
 | Fayssal Bazzi als Mr. Tod (voice) |
 | Ewen Leslie als Pigling Bland (voice) |
 | Christian Gazal als Felix D'eer (voice) |
 | Rachel Ward als Josephine Rabbit (voice) |
 | Bryan Brown als Mr. Rabbit (voice) |
 | David Wenham als Johnny Town-Mouse (voice) |
 | Will Reichelt als JW Rooster II (voice) |
 | Ming-Zhu Hii als Sarabeth |
| Lance Kerfuffle als J. David Scarfington |
 | Natalie Dew als Janelle |
| Terenia Edwards als Siobhan |
| Gareth Davies als Bannerman |
 | Vauxhall Jermaine als Harrods Security |
| Tom Greaves als Harrods Security |
 | Sacha Horler als Betty |
 | Alex Blias als Phil |
 | Dave Lawson als Chris |
 | Sam Haft als Postman |
 | Jessica Friedman als Singing Sparrow (voice) |
| Shana Halligan als Singing Sparrow (voice) |
| Katharine Hoye als Singing Sparrow (voice) |
 | Chris Mann als Singing Sparrow (voice) |
| Chad Reisser als Singing Sparrow (voice) |
 | Fletcher Sheridan als Singing Sparrow (voice) |
| Alexandra Gluck als London Street Rat (voice) |
| Taryn Gluck als London Street Rat (voice) |
| Will Gluck | Writer |
| Will Gluck | Director |
| Beatrix Potter | Book |
| Rob Lieber | Writer |
| Zareh Nalbandian | Producer |
| Peter Menzies Jr. | Director of Photography |
| Christian Gazal | Editor |
| Will Gluck | Producer |
| Brian Lynch | Associate Producer |
| David Williams | Lead Animator |
| Sara Desmond | Unit Production Manager |
| Chris Upson | Visual Effects |
| Rob Coleman | Animation Director |
| Lawrence Woodward | Stunt Coordinator |
| Michael Furniss | Visual Effects |
| P.J. Voeten | First Assistant Director |
| Andrew Power | Second Unit First Assistant Director |
| Danielle Blake | Second Second Assistant Director |
| Xander Collier | Assistant Director |
| Zoe Liang | Second Assistant Director |
| Christine Luby | Third Assistant Director |
| Christian Otty | Second Assistant Director |
| Barney Shakespeare | Third Assistant Director |
| Richard Bradshaw | "B" Camera Operator |
| David Churchyard | First Assistant "B" Camera |
| Rebecca Crowe | First Assistant Camera |
| Inaki De Ubago | Second Assistant Camera |
| Simon Dunn | Second Assistant "A" Camera |
| David Elmes | First Assistant "A" Camera |
| Frank Flick | "A" Camera Operator |
| Ian Jackson | Second Assistant "A" Camera |
| Andrew Jones | Second Assistant "B" Camera |
| Gerard Maher | First Assistant "B" Camera |
| Jack Mayo | First Assistant "B" Camera |
| Jac Norton | Second Assistant "B" Camera |
| Lorena Pagés | Second Assistant Camera |
| Adrien Seffrin | First Assistant Camera |
| Naomi Sharp | Second Assistant "A" Camera |
| Matt Windon | First Assistant "A" Camera |
| Charmian Gradwell | Dialect Coach |
| Sally Hague | Dialect Coach |
| Dominic Lewis | Original Music Composer |
