 | Jessica Alba als Marissa Wilson |
 | Joel McHale als Wilbur Wilson |
 | Rowan Blanchard als Rebecca Wilson |
 | Mason Cook als Cecil Wilson |
 | Jeremy Piven als Danger D'Amo / Tick Tock / Time Keeper |
 | Alexa PenaVega als Carmen Cortez |
 | Daryl Sabara als Juni Cortez |
 | Danny Trejo als Uncle Machete |
 | Belle Solorzano als Spy Baby |
| Genny Solorzano als Spy Baby |
 | Ricky Gervais als Voice of Argonaut |
| Elmo als Argonaut |
| Jett Good als Young Danger |
| Chuck Cureau als News Anchor |
| Albert Im als Head Scientist |
 | Wray Crawford als Editor / Cameraman |
 | Jonathan Breck als Wilbur's Boss |
| Alcides Dias als OSS Agent #1 |
 | Roger Edwards als OSS Agent #2 |
 | Angela Lanza als Female OSS Agent |
 | Chad Guerrero als Henchman in Van |
| Marci Madison als Agent Fine |
 | Antonio Banderas als Gregorio Cortez (uncredited) |
| Robert Rodriguez | Writer |
| Robert Rodriguez | Director |
| David Hack | Set Decoration |
| Robert Rodriguez | Director of Photography |
| George Huang | Co-Producer |
| Robert Rodriguez | Music |
| Steve Joyner | Production Design |
| Carl Thiel | Music |
| Dan Zimmerman | Editor |
| J.C. Cantu | Casting |
| Rebecca Rodriguez | Co-Producer |
| Mary Vernieu | Casting |
| Bob Weinstein | Executive Producer |
| Tom Proper | Associate Producer |
| Elizabeth Avellan | Producer |
| Harvey Weinstein | Executive Producer |
| Caylah Eddleblute | Production Design |
| Nina Proctor | Costume Design |
| Jimmy Lindsey | Director of Photography |
| Robert Rodriguez | Producer |
| Bill Scott | Line Producer |
| Jackson Spidell | Stunts |
| Alex Toader | Visual Effects Supervisor |
| Lauren Andersen | Makeup Artist |
| Howard Berger | Makeup Artist |
| Becki Drake | Makeup Artist |
| Jake Garber | Makeup Artist |
| Ermahn Ospina | Makeup Department Head |
| Robert Ramos | Hairstylist |
| Greg Nicotero | Special Effects Makeup Artist |
| Joe Rivera | Key Hair Stylist |
| Elizabeth Kushman | Executive In Charge Of Production |
| Matthew Stein | Executive In Charge Of Production |
| Matt Landon | Executive In Charge Of Post Production |
| Brian Bettwy | First Assistant Director |
| Vanessa Gaitan | Second Second Assistant Director |
| David Rimer | Second Assistant Director |
| Jeff B. Adams Jr. | Set Designer |
| Paul Alix | Concept Artist |
| Steve Allen | Propmaker |
| Ted Altman | Props |
| Marc Baird | Storyboard Artist |
| Edward J. Borasch Jr | Property Master |
| Jarid Boyce | Storyboard Artist |
| Jessica Bradley | Art Department Production Assistant |
| Marcia Bruscato-Poss | Construction Buyer |
| Brandon Campbell | Sculptor |
| Corey Childress | Carpenter |
| Marc Dabrusin | Greensman |
| Jaime Fernandez | Assistant Property Master |
| Ken Gaston-Kilgore | Set Dresser |
| Mark Hanks | Leadman |
| Chad Stahelski | Stunts |
| Steve M. Davison | Stunt Coordinator |
| Jeffrey J. Dashnaw | Stunt Coordinator |
