 | William Shatner als Captain James T. Kirk |
 | Leonard Nimoy als Captain Spock |
 | DeForest Kelley als Dr. Leonard 'Bones' McCoy |
 | James Doohan als Captain Montgomery 'Scotty' Scott |
 | George Takei als Captain Hikaru Sulu |
 | Walter Koenig als Cmdr. Pavel Chekov |
 | Nichelle Nichols als Cmdr. Uhura |
 | Christopher Plummer als General Chang |
 | Mark Lenard als Sarek |
 | David Warner als Chancellor Gorkon |
 | Kim Cattrall als Lieutenant Valeris |
 | Rosanna DeSoto als Azetbur |
 | Kurtwood Smith als Federation President |
 | Brock Peters als Admiral Cartwright |
 | Paul Rossilli als Kerla |
 | John Schuck als Klingon Ambassador |
 | Iman als Martia |
 | Leon Russom als Chief in Command |
 | Michael Dorn als Klingon Defense Attorney |
 | Robert Easton als Klingon Judge |
 | Grace Lee Whitney als Excelsior Communications Officer (Janice Rand) |
 | Christian Slater als Excelsior Communications Officer |
 | Jeremy Roberts als Excelsior Officer |
 | Darryl Henriques als Nanclus |
 | Matthias Hues als Second Klingon General |
| Clifford Shegog als Klingon Officer |
 | William Morgan Sheppard als Klingon Commander |
 | Brett Porter als General Stex |
 | Angelo Tiffe als Excelsior Navigator |
 | Boris Lee Krutonog als Helmsman Lojur |
 | Tom Morga als Brute |
 | Todd Bryant als Klingon Translator |
 | John Bloom als Behemoth Alien |
| Jim Boeke als Klingon General |
| Michael Snyder als Crewman Dax |
 | Denise Lynne Roberts als Bridge Background (uncredited) |
| Nicholas Meyer | Director |
| Steven-Charles Jaffe | Producer |
| Ralph Winter | Producer |
| Nicholas Meyer | Screenplay |
| Denny Martin Flinn | Screenplay |
| Hiro Narita | Director of Photography |
| Nilo Rodis-Jamero | Art Direction |
| Mickey S. Michaels | Set Decoration |
| Dodie Shepard | Costume Design |
| Cliff Eidelman | Original Music Composer |
| Herman F. Zimmerman | Production Design |
| Mary Jo Slater | Casting |
| William Hoy | Editor |
| Ronald Roose | Editor |
| Michael Key | Makeup Artist |
| Leonard Nimoy | Executive Producer |
| Brooke Breton | Associate Producer |
| Janice Alexander | Hairstylist |
| R. Christopher Biggs | Special Effects Makeup Artist |
| Mary Burton | Makeup Artist |
| Ed French | Special Effects Makeup Artist |
| Art Harding | Makeup Artist |
| Tina Hoffman | Makeup Artist |
| Nancy J. Hvasta Leonardi | Assistant Makeup Artist |
| Dayne Johnson | Prosthetic Makeup Artist |
| Dean Jones | Makeup Artist |
| Karen Kubeck | Assistant Makeup Artist |
| Cat'Ania McCoy-Howze | Special Effects Makeup Artist |
| Michael Mills | Makeup Supervisor |
| Gilbert A. Mosko | Makeup Artist |
| Kenny Myers | Makeup Department Head |
| Carol A. O'Connell | Hairstylist |
| Gerald Quist | Makeup Artist |
| Rick Stratton | Makeup Artist |
| Wayne Toth | Special Effects Makeup Artist |
| Todd Tucker | Special Effects Makeup Artist |
| Ron Walters | Makeup Artist |
| Marty Hornstein | Unit Production Manager |
| Katy Garretson | Second Assistant Director |
| Christopher T. Gerrity | Assistant Director |
| Steven-Charles Jaffe | Second Unit Director |
| Randy Suhr | Second Second Assistant Director |
| Douglas E. Wise | First Assistant Director |
| Richard J. Bayard | Construction Coordinator |
| Richard Beck | Assistant Property Master |
| Cliff Bergman | Construction Foreman |
| Cliff Boulé | Graphic Designer |
| Joseph Conti | Graphic Designer |
| Edward G. Fitzgerald | Leadman |
| Don Hulett | Property Master |
| Alan S. Kaye | Set Designer |
| Iain McCaig | Storyboard Artist |
| Louise Nielsen | Set Designer |
| Gene Nollman | Set Designer |
| Phillip Norwood | Storyboard Artist |
| Michael Okuda | Graphic Designer |
| Robert Van Dyke | Propmaker |
| Ron Wilkinson | Set Designer |
| Bobbi Banks | ADR Editor |
| Bob Baron | ADR Mixer |
| Bruce Bell | Sound Editor |
| Steve Cantamessa | Boom Operator |
| James Cavarretta | Sound Recordist |
| Greg Curda | Foley Mixer |
| Ken Dufva | Foley Artist |
| David Lee Fein | Foley Artist |
| Marva Fucci | Assistant Sound Editor |
| Thomas A. Fucci | Sound Editor |
| Jeffrey J. Haboush | Sound Re-Recording Mixer |
| Mike Haney | Sound Recordist |
| Matthew Harrison | Foley Editor |
| Michael Herbick | Sound Re-Recording Mixer |
| Frank Howard | Sound Editor |
| Suhail Kafity | Sound Editor |
| Victoria Martin | Foley Editor |
| F. Hudson Miller | Supervising Sound Editor |
| R.J. Palmer | Sound Editor |
| Gary Ritchie | Sound Recordist |
| Greg P. Russell | Sound Re-Recording Mixer |
| Fred Stafford | ADR Editor |
| George Watters II | Supervising Sound Editor |
| Butch Wolf | Foley Editor |
| Jack Woods | Sound Editor |
| Terry D. Frazee | Special Effects Supervisor |
| Don Pike | Stunt Coordinator |
| Eric Anderson | Camera Operator |
| Jeff Case | Dolly Grip |
| John V. Fante | Second Unit Director of Photography |
| Kristin R. Glover | Camera Operator |
| Rob Morey | First Assistant Camera |
| Raman Rao | Chief Lighting Technician |
| Gregory Schwartz | Still Photographer |
| Jan Glaser | Casting Associate |
| Christine Heinz | Key Costumer |
| Joseph Markham | Key Costumer |
| Elaine Maser | Costume Supervisor |
| Scott Caldwell | Assistant Editor |
| Daniel Candib | Assistant Editor |
| Dale E. Grahn | Color Timer |
| Theresa Repola Mohammed | Negative Cutter |
| Rhonda Baer | Location Manager |
| Bunny Andrews | Music Editor |
| Cliff Eidelman | Conductor |
| Faye Brenner | Script Supervisor |
| Andrea Walzer | Script Supervisor |
| Timothy Greenwood | Projection |
| Bob Hoffman | Unit Publicist |
| David Oliver Pfeil | Title Designer |
| Brian Wensel | Production Accountant |
| Gene Roddenberry | In Memory Of |
| Steve Neill | Special Effects Makeup Artist |
| Cole S. McKay | Stunts |
| Jeff Imada | Stunts |
| Terry D. Frazee | Special Effects |
| Don Pulford | Stunt Double |
| Erik Stabenau | Stunts |
| Gary Baxley | Stunts |
| Ed Anders | Stunts |
| Jeff Bornstein | Stunts |
| Hal Burton | Stunts |
| Charlie Brewer | Stunts |
| Joe Farago | Stunts |
| BJ Davis | Stunts |
| Joy Hooper | Stunts |
| Tommy J. Huff | Stunts |
| Rob King | Stunts |
| Scott Leva | Stunts |
| Jeffrey Scott Jensen | Stunts |
| Eric Norris | Stunts |
| Noon Orsatti | Stunts |
| Alan Marcus | Stunts |
| Gary Pike | Stunts |
| Pat Romano | Stunts |
| Danny Rogers | Stunts |
| Don Ruffin | Stunts |
| Spike Silver | Stunts |
| Joyce Agu | Stunt Double |
