 | Andrew Garfield als Spider-Man / Peter Parker |
 | Emma Stone als Gwen Stacy |
 | Rhys Ifans als The Lizard / Dr. Curt Connors |
 | Denis Leary als Captain Stacy |
 | Martin Sheen als Uncle Ben |
 | Sally Field als Aunt May |
 | Irrfan Khan als Rajit Ratha |
 | Campbell Scott als Richard Parker |
 | Embeth Davidtz als Mary Parker |
 | Chris Zylka als Flash Thompson |
 | Max Charles als Peter Parker (Age 4) |
 | C. Thomas Howell als Jack's Father |
 | Jake Keiffer als Jack |
 | Kari Coleman als Helen Stacy |
 | Stan Lee als School Librarian |
 | Hannah Marks als Missy Kallenback |
 | Kelsey Chow als Hot Girl |
 | Kevin McCorkle als Mr. Cramer |
 | Barbara Eve Harris als Miss Ritter |
 | Danielle Burgio als Nicky's Girlfriend |
 | Michael Barra als Store Clerk |
 | Leif Gantvoort als Cash Register Thief |
 | Andy Pessoa als Gordon |
 | Andy Gladbach als Physics Nerd |
 | Ring Hendricks-Tellefsen als Physics Nerd |
 | Tom Waite als Nicky |
 | Keith Campbell als Car Thief |
 | Steve DeCastro als Car Thief Cop |
 | James Chen als olice Officer |
| Cuitlahuac Morales Velazquez | Sculptor |
| Abe Costanza | Scenic Artist |
| Eric Sundahl | Set Designer |
| Shannon Mills | Supervising Sound Editor |
| Alvin Sargent | Screenplay |
| Steve Kloves | Screenplay |
| James Vanderbilt | Story |
| Stan Lee | Characters |
| Steve Ditko | Characters |
| Francine Maisler | Casting |
| J. Michael Riva | Production Design |
| Page Buckner | Art Direction |
| Michael E. Goldman | Art Direction |
| David F. Klassen | Supervising Art Director |
| Suzan Wexler | Art Direction |
| Leslie A. Pope | Set Decoration |
| Lynda Foote | Costume Supervisor |
| Susan J. Wright | Costume Supervisor |
| James Vanderbilt | Screenplay |
| Marc Webb | Director |
| James Horner | Original Music Composer |
| John Schwartzman | Director of Photography |
| Alan Edward Bell | Editor |
| Pietro Scalia | Editor |
| Stan Lee | Executive Producer |
| Kevin Feige | Executive Producer |
| Michael Grillo | Executive Producer |
| Laura Ziskin | Producer |
| Avi Arad | Producer |
| Matt Tolmach | Producer |
| Kym Barrett | Costume Design |
| Vincent Schicchi | Makeup Department Head |
| Ve Neill | Makeup Department Head |
| Andrew Birdzell | Set Designer |
| Ernie Avila | Set Designer |
| Andrew M. Siegel | Property Master |
| Michelle Collier | Art Department Coordinator |
| Joseph A. Alfieri Jr. | Construction Coordinator |
| Joseph S. Alfieri | Construction Coordinator |
| Russell R. Anderson | Leadman |
| Eric Hugunin | Assistant Art Director |
| Nancy A. King | Art Department Coordinator |
| Miguel López-Castillo | Assistant Art Director |
| Paul Sonski | Assistant Art Director |
| Noelle King | Set Designer |
| William J. Law III | Set Designer |
| Thomas Machan | Set Designer |
| Shannon Mills | Sound Designer |
| Addison Teague | Supervising Sound Editor |
| Addison Teague | Sound Designer |
| Robert Troy | Dialogue Editor |
| Robin Harlan | Foley |
| Sarah Monat | Foley |
| Deb Adair | Sound Re-Recording Mixer |
| David Giammarco | Sound Re-Recording Mixer |
| Paul Massey | Sound Re-Recording Mixer |
| Patrick Ballin | Visual Effects Editor |
| Ashley Beck | Visual Effects Supervisor |
| John Berri | Visual Effects Editor |
| Terry Bradley | Visual Effects Supervisor |
| Jerome Chen | Visual Effects Supervisor |
| Kevin Crandell | Visual Effects Editor |
| Joe Farrell | Visual Effects Supervisor |
| Mark Herman | Visual Effects Editor |
| Sébastien Moreau | Visual Effects Supervisor |
| Sascha Wolff | Visual Effects Editor |
| Lindsey Zamplas | Visual Effects Producer |
| Jennifer Silver | Visual Effects Producer |
| Julie Orosz | Visual Effects Producer |
| Julia Neighly | Visual Effects Producer |
| Hetal Jain | Visual Effects Producer |
| Matt Daly | Visual Effects Producer |
| John Clinton | Visual Effects Producer |
| Andy Armstrong | Stunt Coordinator |
| James Armstrong | Stunt Coordinator |
| David R. Christensen | Gaffer |
| Russell Engels | Gaffer |
| Ian Fox | Camera Operator |
| David Luckenbach | Camera Operator |
| David Luckenbach | Steadicam Operator |
| Peter Mercurio | Camera Operator |
| Michael Nelson | Camera Operator |
| Jaimie Trueblood | Still Photographer |
| Valentina Aulisi | Set Costumer |
| Paul Corricelli | Set Costumer |
| David Fernandez | Set Costumer |
| Elizabeth Frank | Set Costumer |
| Mustapha Mimis | Set Costumer |
| Bob Moore Jr. | Set Costumer |
| Julie Shack | Set Costumer |
| Malik Summers | Set Costumer |
| Jim Henrikson | Music Editor |
| Barbara McDermott | Music Editor |
| Joe E. Rand | Music Editor |
| Joel Marrow | Transportation Coordinator |
| Trisha Burton | Script Supervisor |
| Elizabeth Himelstein | Dialect Coach |
