 | Andrew Garfield als Peter Parker |
 | Emma Stone als Gwen Stacy |
 | Martin Sheen als Uncle Ben |
 | Sally Field als Aunt May |
 | Rhys Ifans als Dr. Curt Connors/ Lizard |
 | Denis Leary als Capt. George Stacy |
 | Irrfan Khan als Dr. Ratha |
 | Embeth Davidtz als Mary Parker |
| James Vanderbilt | Screenplay |
| Marc Webb | Director |
| James Horner | Original Music Composer |
| John Schwartzman | Director of Photography |
| Alan Edward Bell | Editor |
| Pietro Scalia | Editor |
| Stan Lee | Executive Producer |
| Kevin Feige | Executive Producer |
| Michael Grillo | Executive Producer |
| Laura Ziskin | Producer |
| Avi Arad | Producer |
| Matt Tolmach | Producer |
| Kym Barrett | Costume Design |
| Leslie A. Pope | Costume Design |
| Ve Neill | Makeup Department Head |
