 | Andrew Garfield als Spider-Man / Peter Parker |
 | Emma Stone als Gwen Stacy |
 | Jamie Foxx als Electro / Max Dillon |
 | Dane DeHaan als Green Goblin / Harry Osborn |
 | Colm Feore als Donald Menken |
 | Felicity Jones als Felicia |
 | Paul Giamatti als Aleksei Sytsevich |
 | Sally Field als Aunt May |
 | Embeth Davidtz als Mary Parker |
 | Campbell Scott als Richard Parker |
 | Marton Csokas als Dr. Ashley Kafka |
 | Louis Cancelmi als Man in Black Suit |
 | Max Charles als Young Peter Parker |
 | Sarah Gadon als Kari |
 | Stan Lee als Graduation Guest |
 | Chris Cooper als Norman Osborn |
 | Frank Deal als Agent Berkley |
 | Denis Leary als Captain Stacy |
 | Martin Sheen als Uncle Ben |
 | Chris Zylka als Flash Thompson |
 | B. J. Novak als Allistar Smythe |
| Marc Webb | Director |
| Avi Arad | Producer |
| Matt Tolmach | Producer |
| Mark Friedberg | Production Design |
| Pietro Scalia | Editor |
| Deborah Lynn Scott | Costume Design |
| Alex Kurtzman | Screenplay |
| Roberto Orci | Screenplay |
| Jeff Pinkner | Screenplay |
| James Vanderbilt | Story |
| Daniel Mindel | Director of Photography |
| Ve Neill | Makeup Artist |
| Alex Kurtzman | Story |
| Roberto Orci | Story |
| Jeff Pinkner | Story |
| Stan Lee | Characters |
| Steve Ditko | Characters |
| Nevin Seus | Music Editor |
| Johnny Marr | Original Music Composer |
| Hans Zimmer | Original Music Composer |
| Pharrell Williams | Original Music Composer |
| Kathleen Chopin | Casting |
| Kim Jennings | Art Direction |
| Richard L. Johnson | Supervising Art Director |
| Susan Bode | Set Decoration |
| John Caglione Jr. | Makeup Department Head |
| Stephen M. Kelley | Makeup Department Head |
| Joseph S. Alfieri | Construction Coordinator |
| Tim Croshaw | Set Designer |
| Sandy Hamilton | Property Master |
| Noelle King | Set Designer |
| Douglas Poland | Art Department Coordinator |
| Tim Zydel | Greensman |
| David V. Butler | Dialogue Editor |
| Sean Massey | Dialogue Editor |
| Robert Troy | Dialogue Editor |
| Luis Galdames | Sound Effects Editor |
| Lee Gilmore | Sound Effects Editor |
| Hamilton Sterling | Sound Effects Editor |
| Robin Harlan | Foley |
| Sarah Monat | Foley |
| Kira Smith | Boom Operator |
| Addison Teague | Supervising Sound Editor |
| Eric A. Norris | Sound Designer |
| Joe Digaetano | Special Effects Coordinator |
| Ashley Beck | Visual Effects Supervisor |
| Don Lee | Visual Effects Supervisor |
| Kim Lee | Visual Effects Producer |
| Gregory L. McMurry | Visual Effects Supervisor |
| Andy Armstrong | Stunt Coordinator |
| James Armstrong | Stunt Coordinator |
| Nicole Greenbaum | Costume Supervisor |
| Lisa Lovaas | Costume Supervisor |
| Donna Maloney | Costume Supervisor |
| James Fanning | Transportation Captain |
| Donna Belsky | Studio Teachers |
| Jason Farrar | Location Manager |
| Eric Lewis Beauzay | Assistant Art Director |
| Ryan Heck | Assistant Art Director |
| Malchus Janocko | Assistant Art Director |
| Stephen Morahan | Assistant Art Director |
| Rachel Nemec | Assistant Art Director |
| Mark Pollard | Assistant Art Director |
| Matthew Jeffrey Sama | Assistant Art Director |
| David Stein | Assistant Art Director |
| Joseph Michael Tenga | Assistant Art Director |
| Scott Dougan | Assistant Art Director |
| Marc Connor | Scenic Artist |
| Eric de Jesus | Scenic Artist |
| Max Grafe | Scenic Artist |
| Christopher Kay | Scenic Artist |
| Kathryn C. McGinnis | Scenic Artist |
| Diana Shaller | Scenic Artist |
| Bruce Lee Gross | Leadman |
| Linda Drake | Visual Effects Editor |
| Mark Herman | Visual Effects Editor |
| Philippe Carr-Forster | Camera Operator |
| Ted Chu | Camera Operator |
| Petr Hlinomaz | Camera Operator |
| William Klayer | Camera Operator |
| George Peters | Camera Operator |
| Thomas Lappin | Camera Operator |
| Christopher Prampin | Gaffer |
| Stephen Pardee | Set Costumer |
| Laura Wolford | Set Costumer |
| Deborah Zimmerman | Set Costumer |
| Mandy Arnold | Digital Intermediate |
| Elizabeth Himelstein | Dialect Coach |
| Julie Kuehndorf | Publicist |
| Wendy Lee Roberts | Script Supervisor |
| Daniel Carbo | Production Manager |
| Denise Pinckley | Production Manager |
| Angela Quiles | Production Supervisor |
