 | Ben Stiller als Larry Daley / Laaa |
 | Robin Williams als Theodore Roosevelt |
 | Owen Wilson als Jedediah Smith |
 | Steve Coogan als Octavius |
 | Ricky Gervais als Dr. McPhee |
 | Dan Stevens als Sir Lancelot |
 | Rebel Wilson als Tilly |
 | Skyler Gisondo als Nick Daley |
 | Rami Malek als Ahkmenrah |
 | Patrick Gallagher als Attila the Hun |
 | Mizuo Peck als Sacajawea |
 | Ben Kingsley als Merenkahre |
 | Dick Van Dyke als Cecil |
 | Mickey Rooney als Gus |
 | Bill Cobbs als Reginald |
 | Andrea Martin als Rose |
 | Rachael Harris als Madeline Phelps |
 | Matt Frewer als Archibald Stanley |
 | Brad Garrett als Easter Island Head (voice) |
 | Regina Taufen als New York Reporter (voice) |
 | Percy Hynes White als C.J. Fredericks |
 | Brennan Elliott als Robert Fredericks |
 | Kerry van der Griend als Neanderthal #1 |
 | Matthew Harrison als Neanderthal #2 |
 | Jody Racicot als Neanderthal #3 |
 | Randy Lee als Hun #1 |
 | Darryl Quon als Hun #2 |
 | Paul Chih-Ping Cheng als Hun #3 |
 | Gerald Wong als Hun #4 |
 | Anjali Jay als Shepseheret |
 | Matty Finochio als Roman Sentry |
 | Patrick Sabongui als Amir |
 | Amir M. Korangy als 1938 Egyptian Worker |
 | Louriza Tronco als Andrea |
 | Sophie Levy als Sophie |
 | Jin Sangha als Egyptian High Priest |
 | James Neate als Augustus Statue |
 | Alice Eve als Alice Eve (uncredited) |
 | Hugh Jackman als Hugh Jackman (uncredited) |
 | Kishore Bhatt als Theatre Patron (uncredited) |
 | Dmitrious Bistrevsky als Greek Statue (uncredited) |
| Hannah Blamires als Theatre Patron (uncredited) |
 | Jill Buchanan als Passerby (uncredited) |
 | Arpit Chaudhary als Theatre Patron (uncredited) |
 | Michael Denis als Young Ahkmenrah's Father (uncredited) |
 | Theo Devaney als Lord Nelson (uncredited) |
| John Dryden als Mummy (uncredited) |
| Jordan Gardiner als Wealthy Donor (uncredited) |
| Ian Hawes als Gala Photographer (uncredited) |
 | Nils Hognestad als Cowboy (uncredited) |
 | Tony Honickberg als Theatre Patron (uncredited) |
| Todor Jordanov als Museum Guard (uncredited) |
 | Zivile Kaminskaite als Gala Event Wealthy Donor (uncredited) |
| Shaun Lucas als Museum Patron / Passerby (uncredited) |
| Sid Man als Passerby (uncredited) |
 | Craig March als NY Cop (uncredited) |
 | Stuart Matthews als Theatre Patron (uncredited) |
 | Martyn Mayger als Action Car Driver (uncredited) |
 | Doris McCarthy als Wealthy Donor (uncredited) |
| Pete Meads als Passerby (uncredited) |
| Leah Perkins als BMW Driver (uncredited) |
 | Jacqueline Ryan als Wealthy Donor (uncredited) |
 | Andrea Sandell als Passerby (uncredited) |
 | Steve Saunders als Passerby (uncredited) |
| Bomber Hurley-Smith als Roman Sentry (uncredited) |
| Celeste White Steele als Theater Attendee (uncredited) |
 | Ella Stiller als Child on London Bus (uncredited) |
 | Quinn Dempsey Stiller als Child on London Bus (uncredited) |
| Winson Ting als Man (uncredited) |
| Stefano Villabona als Gala Photographer (uncredited) |
 | Seth Whittaker als Museum Guard (uncredited) |
| Buppha Witt als Passerby (uncredited) |
| Brian Woodward als Theatre Patron (uncredited) |
 | Crystal the Monkey als Dexter |
| Shawn Levy | Director |
| Thomas Lennon | Screenplay |
| David Guion | Screenplay |
| Michael Handelman | Screenplay |
| Milan Trenc | Characters |
| Shawn Levy | Producer |
| Josh McLaglen | Executive Producer |
| Mary McLaglen | Executive Producer |
| Alan Silvestri | Music |
| Guillermo Navarro | Director of Photography |
| Dean Zimmerman | Editor |
| Robert Ben Garant | Screenplay |
| Chris Columbus | Producer |
| Mark Radcliffe | Producer |
| Heike Brandstatter | Casting |
| Coreen Mayrs | Casting |
| Martin Whist | Production Design |
| Ravi Bansal | Art Direction |
| Nigel Evans | Art Direction |
| Catherine Ircha | Art Direction |
| Peter Lando | Set Decoration |
| Marlene Stewart | Costume Design |
| Naomi Bakstad | Key Makeup Artist |
| Don Kozma | Makeup Department Head |
| Alla Kornilov | Makeup Artist |
| Nuria Mbomio | Makeup Artist |
| Liliana Meyrick | Hair Department Head |
| Sarah Bergeest Still | Makeup Artist |
| Josh McLaglen | First Assistant Director |
| Frédéric Amblard | Set Designer |
| Kris Bergthorson | Set Designer |
| Aric Cheng | Set Designer |
| Sarah Contant | Set Designer |
| David Dowling | Property Master |
| Houman Eshraghi | Set Designer |
| Matthew Gatlin | Set Designer |
| Douglas Jang | Construction Foreman |
| Rick Kramer | Greensman |
| Brent Lambert | Set Designer |
| Brian Shell | Construction Coordinator |
| Lucie Tremblay | Set Designer |
| Trinh Vu | Set Designer |
| Ginny Walsh | Greensman |
| Carie Wallis | Art Department Coordinator |
| Ralph Sarabia | Painter |
| Gwendolyn Margetson | Set Designer |
| Chris Hopkins | Sculptor |
| Dan Engle | Sculptor |
| Victor James Martinez | Conceptual Design |
| Jean-Paul Costaz | Painter |
| Martin P. Blaine | Carpenter |
| Coll Anderson | Sound Effects Editor |
| Scott Curtis | Foley Editor |
| Susan Dawes | Dialogue Editor |
| Matthew Harrison | Foley Editor |
| Warren Hendriks | Sound Designer |
| Craig Henighan | Supervising Sound Editor |
| Jon Lavender | Boom Operator |
| Michael Asiman | Visual Effects Coordinator |
| Ashley Bellm | Visual Effects Coordinator |
| Hugh Brompton | Visual Effects Coordinator |
| Sirius Buisson | Lighting Director |
| Lesly Cazares | Visual Effects Coordinator |
| Satyaki Paul Choudhury | Lighting Director |
| Ann Chow | Visual Effects Coordinator |
| Monette Dubin | Visual Effects Producer |
| Jamie Erickson | Visual Effects Coordinator |
| Olivia Evelyn | Visual Effects Coordinator |
| Erin Fernie | Production Coordinator |
| Ty Gibson | Visual Effects Editor |
| David Gullman | Visual Effects Coordinator |
| Wahid Ibn Reza | Visual Effects Coordinator |
| Manon Ih | Visual Effects Coordinator |
| Dana Jurcic | Visual Effects Coordinator |
| Rakesh Kali | Visual Effects Coordinator |
| Andrea Kistler | Visual Effects Coordinator |
| Viet Luu | Visual Effects Supervisor |
| Melody Mead | Visual Effects Coordinator |
| Jennifer Meislohn | Visual Effects Producer |
| Derrick Mitchell | Visual Effects Editor |
| Erik Nash | Visual Effects Supervisor |
| Joana Powell | Visual Effects Coordinator |
| Alex Reinach | Visual Effects Coordinator |
| Danny Singh | VFX Editor |
| Laura Schultz | Visual Effects Producer |
| Michael Struk | Visual Effects Editor |
| Paulo Welter | Modeling |
| Brad Martin | Stunt Coordinator |
| Kerry Brown | Still Photographer |
| Mark Chow | Camera Operator |
| Steve Ellingworth | Key Grip |
| Joseph Lederer | Still Photographer |
| John Marzano | Aerial Director of Photography |
| Vince McGahon | Steadicam Operator |
| Ryan Monro | Dolly Grip |
| Grace Delahanty | Seamstress |
| Richard Schoen | Costume Supervisor |
| Jenn Touchie | Seamstress |
| Matthew G. Carson | First Assistant Editor |
| Tyler Ruocco | First Assistant Editor |
| Mark Graham | Orchestrator |
| Kenneth Karman | Supervising Music Editor |
| Rosalie Carew | Script Supervisor |
| Jessica Drake | Dialect Coach |
| Diane Durant | Script Supervisor |
| Samantha Ellison | Script Coordinator |
| Rob Fournier | Armorer |
| Chuck Maldonado | Choreographer |
| Kathy Hubble | Stunt Double |
| Lani Gelera | Stunts |
| Leanne Buchanan | Stunts |
| Celia Bond | Stunts |
| Krista Bell | Stunts |
| Doug Chapman | Stunts |
| Robin Williams | In Memory Of |
| Ferran Domenech | Animation Supervisor |
