 | Keanu Reeves als Kai |
 | Hiroyuki Sanada als Kuranosuke Ôishi |
 | Ko Shibasaki als Mika |
 | Tadanobu Asano als Lord Kira |
 | Min Tanaka als Lord Asano |
 | Rinko Kikuchi als Witch |
 | Jin Akanishi als Chikara |
 | Masayoshi Haneda als Yasuno |
 | Hiroshi Sogabe als Hazama |
 | Takato Yonemoto als Basho |
| Shû Nakajima als Horibe |
 | Hiroshi Yamada als Hara |
 | Cary-Hiroyuki Tagawa als Shogun Tsunayoshi |
 | Tanroh Ishida als Shogun's Adjutant |
 | Yorick van Wageningen als Kapitan |
 | Ron Bottitta als Narrator |
 | Natsuki Kunimoto als Riku |
 | Togo Igawa als Tengu Lord |
 | Akira Koieyama als Kira's Spy |
 | Haruka Abe als Mika's Handmaiden |
 | Clyde Kusatsu als Drunk Official |
 | Junichi Kajioka als Guard |
| Masashi Fujimoto als Lead Soldier |
 | Neil Fingleton als Lovecraftian Samurai |
 | Rick Genest als Foreman |
 | Masayuki Deai als Isogai |
| Carl Rinsch | Director |
| Scott Stuber | Producer |
| Pamela Abdy | Producer |
| Eric McLeod | Producer |
| Chris Morgan | Screenplay |
| Hossein Amini | Screenplay |
| Walter Hamada | Screenstory |
| John Mathieson | Cinematography |
| Ilan Eshkeri | Music |
| Denise Chamian | Casting |
| Priscilla John | Casting |
| Ania O'Hare | Casting |
| Penny Rose | Costume Design |
| Charlotte Finlay | Costume Supervisor |
| Jan Roelfs | Production Design |
| David Allday | Art Direction |
| John Chichester | Art Direction |
| Robert Cowper | Art Direction |
| Fay Greene | Art Direction |
| Stuart Rose | Art Direction |
| Leslie Tomkins | Art Direction |
| Elli Griff | Set Decoration |
| Guy Bradley | Assistant Art Director |
| Gavin Fitch | Assistant Art Director |
| Gary Jopling | Assistant Art Director |
| Zoltán Tóth | Construction Coordinator |
| László Szirmai | Assistant Art Director |
| Slemer Karolina | Art Department Coordinator |
| Jennifer Lewicki | Art Department Coordinator |
| Kevin Cross | Set Designer |
| Miranda Diamond | Set Designer |
| David Doran | Set Designer |
| A. Todd Holland | Set Designer |
| Masako Masuda | Set Designer |
| Suzanna Smith | Set Designer |
| Zoltán Kiss | Sculptor |
| Rohan Harris | Scenic Artist |
| Graeme Purdy | Property Master |
| Codrina Spataru | Sculptor |
| Dan O'Connell | Foley |
| Daniel Laurie | Dialogue Editor |
| Tim Nielsen | Supervising Sound Editor |
| Dean Covill | Sound Designer |
| Dean Covill | Sound Re-Recording Mixer |
| Frank A. Montaño | Sound Re-Recording Mixer |
| Jon Taylor | Sound Re-Recording Mixer |
| Kevin O'Connell | Sound Re-Recording Mixer |
| Nicolas Aithadi | Visual Effects Supervisor |
| Jay Barton | Visual Effects Supervisor |
| Justin Denton | Visual Effects Supervisor |
| Allan Magled | Visual Effects Supervisor |
| Christian Manz | Visual Effects Supervisor |
| Michael Mulholland | Visual Effects Supervisor |
| Tom Woodall | Visual Effects Editor |
| Garv Thorp | Visual Effects Producer |
| Sarah Swick | Visual Effects Producer |
| Lena Scanlan | Visual Effects Producer |
| Tom Partridge | Visual Effects Editor |
| Alain Lalanne | Visual Effects Producer |
| Elise Butt | Visual Effects Editor |
| Stuart M. Ellis | Supervising Animator |
| Rachel Chrisp | Set Costumer |
| Ildikó Fogt | Set Costumer |
| Ian Foweraker | Set Costumer |
| Philip Goldsworthy | Set Costumer |
| Kenneth Karman | Music Editor |
| Julie Pearce | Music Editor |
| Dean Burtenshaw | Transportation Coordinator |
| Claudia Kossik | Transportation Coordinator |
| Rudolf András | Location Manager |
| Bobby White | Dialect Coach |
| Laszlo Rorariusz | Location Manager |
| Olivia Pickford | Choreographer |
| Brian C. Hirono | Dialect Coach |
| Jamie Kemp | Chef |
| Brian N. Bentley | Compositors |
| James Baker | Visual Effects |
| Erik Classen | VFX Artist |
| Gary Freeman | Supervising Art Director |
| Chris Morgan | Screenstory |
| Géza Kovács | Stunts |
| Stephen McLaughlin | Music Score Producer |
| Matthias Gohl | Music Score Producer |
| Andrew Brown | Conductor |
| Kôichi Funayama | Stunts |
| Ken Goto | Stunts |
| Nobuyuki Obikane | Stunts |
| Masahito Okamoto | Stunts |
| Kazuyuki Okayama | Stunts |
| Kazuhiko Ozaki | Stunts |
| Yoshimitsu Tada | Stunts |
| Hiroki Takano | Stunts |
| Ryo Tanaka | Stunts |
| Stuart Baird | Editor |
| Sara Desmond | Production Supervisor |
| Nadia Stacey | Other |
| Chris Fenton | Executive Producer |
| David R. Ellis | Second Unit Director |
| Gary Powell | Stunt Coordinator |
| Fraser Taggart | Second Unit Director of Photography |
| Addison Teague | Sound Designer |
| Walter Hamada | Executive Producer |
