 | Samuel L. Jackson als Afro / Ninja-Ninja (voice) |
 | Lucy Liu als Sio (voice) |
 | Mark Hamill als Bin / Oden Shop Master (voice) |
 | S. Scott Bullock als Professor Dharman / Kidnapper (voice) |
 | Greg Eagles als Afro's Father (voice) |
 | Grey DeLisle als Tomoe / Ogin (voice) |
 | Kevin Michael Richardson als Blacksmith / Takimoto (voice) |
 | Steve Blum als Man A / Assassins (voice) |
 | Dave Wittenberg als Adolescent / Fencing Brother (voice) |
 | Jeff Bennett als Brother 3 (voice) |
 | Liam O'Brien als Shichogoro (voice) |
 | Zachary Gordon als Kotaro (voice) |
 | Phil LaMarr als Young Afro (voice) |
 | Yuri Lowenthal als Jinno (voice) |
 | RZA als DJ (voice) |
 | Ariel Winter als Young Sio (voice) |
| Fuminori Kizaki | Director |
| Shinjiro Mogi | Key Animation |
| Eric S. Calderon | Writers' Production |
| Leo Chu | Writers' Production |
| Samuel L. Jackson | Executive Producer |
| Takashi Okazaki | Comic Book |
| Tomohiro Hirata | Key Animation |
| Kyoko Kotani | Key Animation |
| Tomo Maruyama | Key Animation |
| Yuji Hosogoe | Key Animation |
| Yuuko Yoshida | Key Animation |
| Yoshitaka Yasuda | Key Animation |
| Yoshio Mizumura | Key Animation |
| Yasuko Higuchi | Key Animation |
| Ushio Tazawa | Key Animation |
| Yoshimitsu Ohashi | Key Animation |
| Tomoko Tanaka | Key Animation |
| Tomoko Nitta | Key Animation |
| Tomohiro Kawahara | Key Animation |
| Tetsuo Hirakawa | Key Animation |
| Takashi Kumazen | Key Animation |
| Takako Shimizu | Key Animation |
| Takahiro Takamizawa | Key Animation |
| Takaaki Wada | Key Animation |
| Tadashi Abiru | Key Animation |
