 | Jason Lee als David Seville |
 | David Cross als Ian |
 | Jenny Slate als Zoe |
 | Justin Long als Alvin (voice) |
 | Matthew Gray Gubler als Simon (voice) |
 | Jesse McCartney als Theodore (voice) |
 | Amy Poehler als Eleanor (voice) |
 | Anna Faris als Jeanette (voice) |
 | Christina Applegate als Brittany (voice) |
 | Alan Tudyk als Simone (voice) |
 | Michael P. Northey als Hawaiian Shirt Guy |
 | Sophia Aguiar als Club Woman #1 |
 | Lauren Gottlieb als Club Woman #2 |
 | Tera Perez als Club Woman #3 |
 | Andy Buckley als Captain Correlli |
 | Chad Krowchuk als Waiter |
 | Luisa D'Oliveira als Tessa |
 | Tucker Albrizzi als Tucker / Kite Kid |
 | Nelson Wong als Casino Guy |
| Michael Bagdasarian als Excursion Clerk |
| Amber-Rochelle DeMarco als Damsel #1 |
| Marcela Caceres als Damsel #2 |
| Natalie Sutcliffe als Damsel #3 |
 | Ian Harmon als Monster |
 | Phyllis Smith als Flight Attendant |
 | Ross Bagdasarian, Jr. als Alvin (singing voice) (uncredited) |
 | Janice Karman als Theodore / The Chipettes (singing voice) (uncredited) |
 | Steve Vining als Simon (singing voice) (uncredited) |
| Aaron Atkins als Casino Dealer (uncredited) |
| Eoin Bates als Music Executive (uncredited) |
| Seth Robert Dusky als Additional Voices (voice) (uncredited) |
| Wendell Kinney als Cruise Guest (uncredited) |
| Jeremy Palko als Cruise Guy (uncredited) |
| Rachael Wegener als Model (uncredited) |
| Doug Jackson | Sound Effects Editor |
| Arnon Milchan | Executive Producer |
| Karen Rosenfelt | Executive Producer |
| Mark Mothersbaugh | Original Music Composer |
| Neil A. Machlis | Executive Producer |
| Neil A. Machlis | Unit Production Manager |
| Rose Marie McSherry | Set Decoration |
| Peter Amundson | Editor |
| Casey Grant | Unit Production Manager |
| Lisa Love | Key Makeup Artist |
| Ross Bagdasarian, Jr. | Producer |
| Janice Karman | Producer |
| Thomas E. Ackerman | Director of Photography |
| Allison Jones | Casting |
| Steve Waterman | Executive Producer |
| Richard Holland | Production Design |
| Alexandra Welker | Costume Design |
| David Hadaway | Set Designer |
| David Betancourt | ADR Mixer |
| Galen Goodpaster | Supervising ADR Editor |
| David Lucarelli | ADR Recordist |
| John Murray | Foley Supervisor |
| Charleen Richards | ADR Mixer |
| Glenn Berger | Writer |
| Jonathan Aibel | Writer |
| Mike Mitchell | Director |
| Julia Michels | Music Supervisor |
| Don MacAulay | Art Direction |
| Peter Bodnarus | Set Designer |
| Dan O'Connell | Foley Artist |
| Dan DiPrima | Supervising Music Editor |
| John T. Cucci | Foley Artist |
| Jim Brookshire | Supervising Dialogue Editor |
| Anna Behlmer | Sound Re-Recording Mixer |
| James Bolt | Sound Re-Recording Mixer |
| Stacey Herbert | Makeup Artist |
| Mira Caveno | Set Designer |
| Nico Salvato | First Assistant Sound Editor |
| Sachin Bangera | Animation |
| Aravind Jayaraman | Visual Effects |
| Tony Mecca | Animation |
| Christine Sirois | ADR Recordist |
| Dixie Pizani | Animation |
| Andrew Malesky | Animation |
| Rohit Kolhe | Animation |
| Samidha Nagwekar | Animation |
| Phil Langone | Storyboard |
| Matthew Harrison | Foley Editor |
| John Morris | Supervising Sound Editor |
| Matt Fausak | Music Editor |
| David Husby | Sound Mixer |
| James Ashwill | Foley Mixer |
| Ian Blum | Animation Supervisor |
| Johnny Gidcomb | ADR Voice Casting |
| Melanie Cassidy | Sculptor |
| Jesse Johnstone | ADR Recordist |
