 | Michael Fassbender als Callum Lynch / Aguilar de Nerha |
 | Marion Cotillard als Dr. Sophia Rikkin |
 | Jeremy Irons als Alan Rikkin |
 | Brendan Gleeson als Joseph Lynch |
 | Charlotte Rampling als Ellen Kaye |
 | Michael Kenneth Williams als Moussa |
 | Ariane Labed als Maria |
 | James Sobol Kelly als Father Raymond |
 | Denis Ménochet als Abstergo Güvenlik Müdürü |
 | Callum Turner als Nathan |
 | Khalid Abdalla als Sultan Muhammad XII |
 | Essie Davis als Mary Lynch |
 | Carlos Bardem als Benedicto |
 | Javier Gutiérrez als Tomas de Torquemada |
 | Hovik Keuchkerian als Ojeda |
 | Crystal Clarke als Samia |
 | Brian Gleeson als Young Joseph |
 | Rufus Wright als Alex |
 | Matias Varela als Emir |
 | Coco König als Orderly |
 | Michelle H. Lin als Lin |
 | Julio Jordán als General Ramirez |
 | Angus Brown als Young Cal |
 | Kemaal Deen-Ellis als Prince Ahmed |
 | Aaron Monaghan als Gilles |
 | Thomas Camilleri als King Ferdinand |
 | Marysia S. Peres als Queen Isabella |
 | Gabriel Andreu als Christopher Columbus |
 | Jeff Mash als Prison Warden |
 | Yuric Allison als Muhammad's Official |
 | Joe Kennard als Abstergo Guard |
 | Gertrude Thoma als Speaker |
 | Octavia Selena Alexandru als Lara (uncredited) |
 | Iain Batchelor als Abstergo Guard 1 (uncredited) |
 | Dacio Caballero als Assasin 3 (uncredited) |
 | Emma Clay als Child Chorister (uncredited) |
 | Graham Curry als Templar (uncredited) |
 | Jem Kai Olsen als Templar (uncredited) |
 | Louis Dulanto-Sime als Chorister (uncredited) |
 | Malcolm Ellul als Executioner (uncredited) |
 | Dino Fazzani als Prison Guard (uncredited) |
 | Lee Nicholas Harris als Paramedic (uncredited) |
 | Bruce Lester-Johnson als Prison Guard (uncredited) |
 | Mohammed Ali als Infinity Patient |
| Jade Clarke als Prisoner |
| Alexander Cooper als Passerby |
 | Ty Hurley als Freemason |
 | Matthew Leonhart als Templar |
| Simon Manley als Guard |
 | Juan Carlos Martínez Antuña als Templar |
 | Martyn Mayger als Templar |
 | Stephen McDade als Templar |
 | Anthony Milton als Templar Guard (uncredited) |
 | Ross Moneypenny als Templar |
 | Jassie Mortimer als Female Mason |
 | Cali Nelle als Assassin #2 |
 | Emeson Nwolie als Administration Officer |
| Massimo Portelli als Spanish Crossbowman |
 | Jonathan Pyatt als Spanish Guard |
| Mike Ray als Templar Guest |
| Jonathan Ronin als Spanish Guard |
 | Steve Saunders als Patient |
| Jasper Schellekens als Spanish Infantry |
| Rahji Shrinarine als Templar |
 | Clem So als Animus Patient |
| Clyde Vassallo als Spanish Infantry |
| Sean Vassallo als Spanish Archer |
| Roberto Vivancos als Merchant |
 | Roberto Vivancos als Merchant |
 | Abi Whetton als Choir Member |
 | Christopher Whitlow als Assassin #1 |
 | Cerys Wrigley-Moss als Chorister |
 | Juan Pablo Shuk als Father |
 | Maxime Durand als Spanish Archer (uncredited) |
| Michael Fassbender | Producer |
| Justin Kurzel | Director |
| Paul Gooch | Hair Designer |
| Elizabeth Leslie | VFX Editor |
| Danielle Millington-Peck | VFX Editor |
| Christopher Learmonth | VFX Editor |
| Kuen-Il Song | Foley Editor |
| Jed Kurzel | Music |
| Christopher Tellefsen | Editor |
| Eric Clement | CG Supervisor |
| Michael Lesslie | Screenplay |
| Adam Cooper | Screenplay |
| Bill Collage | Screenplay |
| Markus Barmettler | Executive Producer |
| Jean-Julien Baronnet | Producer |
| Christine Burgess-Quémard | Executive Producer |
| Patrick Crowley | Producer |
| Jean de Rivieres | Executive Producer |
| Daniel Emmerson | Associate Producer |
| Gerard Guillemot | Producer |
| Serge Hascoet | Executive Producer |
| Philip Lee | Executive Producer |
| Frank Marshall | Producer |
| Conor McCaughan | Producer |
| Arnon Milchan | Producer |
| Alex Taylor | Associate Producer |
| Richard Whelan | Associate Producer |
| Jina Jay | Casting |
| Andy Nicholson | Production Design |
| Oliver Carroll | Art Direction |
| David Doran | Art Direction |
| Marc Homes | Supervising Art Director |
| Tom Weaving | Art Direction |
| Matt Wynne | Art Direction |
| Tina Jones | Set Decoration |
| Sammy Sheldon | Costume Design |
| Alison Banks | Production Supervisor |
| Sally French | Unit Production Manager |
| Katie Reynolds | Post Production Supervisor |
| Olivia Brittain | Casting Assistant |
| Andrés Cuenca | Casting Assistant |
| Edward Said | Casting Associate |
| Cesco Bonello | Location Scout |
| Joseph Formosa Randon | Location Manager |
| Rubén Gómez | Location Manager |
| Jacques Mezger | Location Scout |
| Nick Oliver | Location Manager |
| Dan Whitty | Location Manager |
| Abby Mills | Production Coordinator |
| Simon Mills | Production Coordinator |
| Kaitlyn Smith | Production Coordinator |
| Lisa Vick | Script Supervisor |
| David Farrow | Armorer |
| Graeme Purdy | Property Master |
| Thomas Goodwin | Draughtsman |
| Sophie Neil | Draughtsman |
| Tonucha Vidal | Casting |
| Vanessa Baker | ADR Voice Casting |
| Dave Evans | Armorer |
| Adam Arkapaw | Director of Photography |
| Marc Wolff | Aerial Coordinator |
| Lucia Foster Found | Aerial Coordinator |
| Kathleen Shanks | Music Director |
| Poppy Kavanagh | Music Editor |
| Lucy Bright | Music Supervisor |
| Poli Kyriacou | Assistant Costume Designer |
| Robin Jiro Hall | Costume Coordinator |
| Christian Goddard | Set Costumer |
| Geneva Corlett | Set Costumer |
| Marina Angela Ruiz | Set Costumer |
| Sharon McCormack | Tailor |
| Jeremy Braben | Aerial Director of Photography |
| Terence Bulley | Aerial Director of Photography |
| Simon Finney | Camera Operator |
| Simon Baker | Camera Operator |
| Matthew Poynter | Camera Operator |
| Gary Spratling | Camera Operator |
| Lee Walters | Gaffer |
| Nikos Kalimerakis | Gaffer |
| Gavin Walters | Rigging Gaffer |
| Jason Ewart | Steadicam Operator |
| Kerry Brown | Still Photographer |
| Mike Valentine | Underwater Camera |
| Peter Ayriss | Drone Operator |
| Simon Muir | Dolly Grip |
| Alex Coverley | Dolly Grip |
| Paolo Frasson | Dolly Grip |
| Tommaso Mele | Key Grip |
| Massimiliano Dessena | Rigging Grip |
| Marc Wolff | Pilot |
| Zoe Clare Brown | Key Hair Stylist |
| Marcelle Genovese | Key Hair Stylist |
| Julie Kendrick | Key Makeup Artist |
| Julia Vernon | Makeup Supervisor |
| Ann Fenton | Makeup Supervisor |
| Pranay Agarwal | 2D Supervisor |
| Marta Padilla | 3D Modeller |
| Stafford Lawrence | Animation Supervisor |
| Dan Neal | CG Supervisor |
| Sylvain Theroux | CG Supervisor |
| Abbie Kennedy | Matchmove Supervisor |
| Michael Karp | Matchmove Supervisor |
| Adam Coglan | Pre-Visualization Supervisor |
| Mike Hill | Roto Supervisor |
| Adam Heinis | Special Effects Coordinator |
| Dot Kyle | Special Effects Coordinator |
| Andy Williams | Special Effects Supervisor |
| Juan Ramón Molina | Special Effects Supervisor |
| Kenneth Cassar | Special Effects Supervisor |
| Matthew Glen | VFX Editor |
| Lars Andersen | VFX Supervisor |
| Marc Hutchings | VFX Supervisor |
| Sarah Tulloch | Visual Effects Producer |
| Tyler Weiss | Visual Effects Producer |
| David Yrisarri | Visual Effects Producer |
| Kamila Ostra | Visual Effects Producer |
| Tracey Leadbetter | Visual Effects Producer |
| Leila Nicotera | Visual Effects Producer |
| Annie Cliche | Visual Effects Producer |
| Iain Eyre | Dialogue Editor |
| Steve Little | Dialogue Editor |
| James Shannon | Dolby Consultant |
| Carsten Richter | Foley |
| Hanse Warns | Foley |
| Markus Stemler | Sound Designer |
| Alistair Hawkins | Sound Effects Editor |
| Benjamin Krbetschek | Sound Effects Editor |
| Christian Conrad | Sound Effects Editor |
| Sacha Walker | Sound Effects Editor |
| Gilbert Lake | Sound Re-Recording Mixer |
| Steve Single | Sound Re-Recording Mixer |
| Steve Single | Supervising Dialogue Editor |
| Frank Kruse | Supervising Sound Editor |
| Andrew Dearnley | Digital Intermediate |
| Jens Baylis | First Assistant Editor |
| Richard Davies | Assistant Costume Designer |
| Damien Walters | Stunt Double |
| Ben Cooke | Stunt Coordinator |
| Rick English | Stunt Double |
| Jacob Hugghins | Stunts |
| David Hugghins | Stunts |
| Michael Hugghins | Stunts |
| Antal Kalik | Stunt Double |
| Sarah Lochlan | Stunt Double |
| Marek Svitek | Stunts |
| Sara Taddei | Art Department Assistant |
| Ellis Court | Art Department Assistant |
| William McCann | Art Department Assistant |
| Alexia Reveleau | Art Department Assistant |
| Sven Bonnici | Assistant Art Director |
| David Doran | Assistant Art Director |
| Shira Hockman | Assistant Art Director |
| Elizabeth Loach | Assistant Art Director |
| Marco Anton Restivo | Assistant Art Director |
| Ketan Waikar | Assistant Art Director |
| Felicity Hickson | Assistant Art Director |
| Nick Milner | Assistant Property Master |
| Matt Tkocz | Conceptual Design |
| Josue Clotaire Fleurimond | Conceptual Illustrator |
| Jack Dyer | Construction Buyer |
| Dylan Gouder | Construction Coordinator |
| Ray Barrett | Construction Manager |
| Michael Blake | Dressing Prop |
| Tiffany M. Carpenter | Graphic Designer |
| Michael Eaton | Graphic Designer |
| Ash Thorp | Graphic Designer |
| Ian Whiteford | Greensman |
| León Bigiu | Painter |
| Jacqueline Portelli Avellino | Painter |
| Michael Weaver | Painter |
| Jon Banthorpe | Props |
| Richard MacMillan | Props |
| Adam Robinson | Props |
| Terry Taylor | Props |
| Matt Wyles | Props |
| Russell Oxley | Scenic Artist |
| James Dean | Sculptor |
| Codrina Spataru | Sculptor |
| Eleanor Bailey | Set Decorating Coordinator |
| Toby Russell | Set Dresser |
| Ulrich Zeidler | Storyboard Artist |
| Kurt Van Der Basch | Storyboard Artist |
| Darren Hayward | Supervising Carpenter |
| Phil Booth | First Assistant Director |
| Richard Whelan | First Assistant Director |
| Nic Cupac | Best Boy Grip |
| Alex Seery | Digital Imaging Technician |
| James Shovlar | Digital Imaging Technician |
| Joe Steel | Digital Imaging Technician |
| Mardon de Carvalho | Digital Imaging Technician |
| Timo Andert | Digital Imaging Technician |
| Peter Ayriss | Drone Pilot |
| Jamie Bruce | Electrician |
| Ross Busby | Electrician |
| Joe Hissey | Electrician |
| Gary Nolan | Electrician |
| Adam Walker | Electrician |
| Marvin Baldacchino | Electrician |
| Julian Bucknall | First Assistant "A" Camera |
| Spencer Murray | First Assistant "A" Camera |
| Olly Tellett | First Assistant "A" Camera |
| Tobias Eedy | First Assistant "B" Camera |
| Charlie Lia | Key Grip |
| Massimiliano Dessena | Key Rigging Grip |
| Andy Munday | Lighting Programmer |
| Eamonn Fitzgerald | Lighting Technician |
| Thomas Royal | Lighting Technician |
| Paul Snell | Second Assistant "A" Camera |
| Tommy Holman | Second Assistant "B" Camera |
| Dean Murray | Second Assistant "C" Camera |
| Punam Chhatralia | Contact Lens Technician |
| Reenal Shah | Contact Lens Technician |
| Madlen Mierzwiak | Hairstylist |
| Antoinette Aderotoye | Hairstylist |
| Kat Ali | Makeup Artist |
| Sharon Colley | Makeup Artist |
| Natalia Osipova | Makeup Artist |
| Stacy Wodu | Makeup Artist |
| Alex Rouse | Wigmaker |
| Michael Agosta | Costume Assistant |
| Carmen Fenech | Costume Assistant |
| Samuela Galea | Costume Assistant |
| Jessica Lawless | Costume Assistant |
| Billy Prichard | Costume Assistant |
| Claire Mitchell | Principal Costumer |
| Amelia Bianchi | Wardrobe Assistant |
| James Debono | Wardrobe Assistant |
| Tom Scott | Assistant Editor |
| Shay Greenfield | Assistant Editor |
| Justin Tillett | Online Editor |
| Tom Poole | Senior Colorist |
| Chris Keller | 2D Sequence Supervisor |
| Luke Letkey | 2D Sequence Supervisor |
| Chris Gooch | 2D Sequence Supervisor |
| Gruff Owen | Compositing Supervisor |
| Benjamin Ribière | Compositing Supervisor |
| Alex Webb | Compositing Supervisor |
| Jean-Pierre Flayeux | Compositing Supervisor |
| Vincent Poitras | Compositing Supervisor |
| Didier Bertrand | Compositing Supervisor |
| Charlotte Roberts | Creature Technical Director |
| Jack Beynon | Creature Technical Director |
| Avijit Das | Creature Technical Director |
| Adam Vanner | Creature Technical Director |
| Brett Schroeder | Lead Animator |
| Alper Aksit | Lead Animator |
| Nigel Rafter | Lead Animator |
| Roland Yepez | Lead Animator |
| Steve Cady | Lead Animator |
| Janson Chew | Lighting Artist |
| Ian Allard | Lighting Supervisor |
| Nicolas Seck | Senior Animator |
| Kiel Figgins | Senior Animator |
| Philip Cabana | Visual Effects Coordinator |
| Mark Divekar | Visual Effects Coordinator |
| Chris Jestico | Visual Effects Coordinator |
| Nishtha Kantharia | Visual Effects Coordinator |
| Adam Lawrence | Visual Effects Coordinator |
| Alexander Maldoff | Visual Effects Coordinator |
| Nicole Maria Nonis | Visual Effects Coordinator |
| Emma Rider | Visual Effects Coordinator |
| Kasey Simpson | Visual Effects Coordinator |
| Natalia Altavilla | Visual Effects Coordinator |
| Rachel Cadden | Visual Effects Coordinator |
| Balendu Kurup | Visual Effects Coordinator |
| Livi Naylor | Visual Effects Coordinator |
| Megan Louise Smith | Visual Effects Coordinator |
| Mat Thompson | Visual Effects Coordinator |
| Hannah Johnson | Visual Effects Coordinator |
| Marie-Pier Barrette | Visual Effects Coordinator |
| Marie-Claude Lafontaine | Visual Effects Coordinator |
| Louis-Charles Lapointe | Visual Effects Coordinator |
| Joyce Au | Visual Effects Coordinator |
| Joanie Croteau | Visual Effects Coordinator |
| Karina Mariano | Visual Effects Coordinator |
| Deborah Zadzora | Visual Effects Coordinator |
| Samuel Perkins | Visual Effects Editor |
| Sivaprayag | Visual Effects Editor |
| Collette Nunes | Visual Effects Editor |
| Sam Paul Toms | Visual Effects Editor |
| Guillaume Poulin | Visual Effects Editor |
| Samantha Tracey | Visual Effects Production Manager |
| Anita Ho | Visual Effects Production Manager |
| Nairye Apelian | Visual Effects Production Manager |
| Owen E. Williams | Visual Effects Supervisor |
| Ged Wright | Visual Effects Supervisor |
| Justin Cornish | Visual Effects Supervisor |
| Clwyd Edwards | Visual Effects Supervisor |
| Brooke Lyndon-Stanford | Visual Effects Supervisor |
| Christian Irles | Visual Effects Supervisor |
| Joao Sita | Visual Effects Supervisor |
| Sara Bennett | Visual Effects Supervisor |
| Graham Page | Visual Effects Supervisor |
| Dominic Parker | Visual Effects Supervisor |
| Michael Grobe | Visual Effects Supervisor |
| Jean-François Ferland | Visual Effects Supervisor |
| Loeng Wong-Savun | Visual Effects Supervisor |
| Joseph Kasparian | Visual Effects Supervisor |
| Yanick Wilisky | Visual Effects Supervisor |
| Javier Meroño | Visual Effects |
| Katie McDonnell | Stunts |
| Matt Crook | Stunts |
| Caridad Angus | Stand In |
| Nina Jalava | Stand In |
| Jaan Roose | Stunt Double |
| Fraser Taggart | Additional Photography |
| Dan Bradley | Second Unit Director |
| Lucy Cork | Stunt Double |
| Ben Shepherd | Visual Effects |
