 | Kevin Conroy als Batman / Bruce Wayne (voice) |
 | Neal McDonough als Deadshot / Floyd Lawton (voice) |
 | Hynden Walch als Harley Quinn / Harleen Quinzel (voice) |
 | Matthew Gray Gubler als The Riddler / Edward Nigma (voice) |
 | Troy Baker als The Joker (voice) |
 | Eric Bauza als Security Guy (voice) |
 | Chris Cox als G.C.P.D. Commissioner James Gordon (voice) |
 | John DiMaggio als King Shark / Nanaue / Bane (voice) |
 | Greg Ellis als Captain Boomerang (voice) |
 | Giancarlo Esposito als Black Spider / Eric Needham (voice) |
 | Jennifer Hale als Killer Frost / Louise Lincoln (voice) |
 | Martin Jarvis als Alfred Pennyworth (voice) |
 | Peter Jessop als Watch Commander (voice) |
 | Christian Lanz als Scarecrow / Dr. Jonathan Crane / Victor Zsasz (voice) |
 | Nolan North als The Penguin / Oswald Cobblepot / KGBeast / Anatoli Knyazev (voice) |
 | Christopher Lee Parson als Stout Guard (voice) |
 | CCH Pounder als Amanda Waller (voice) |
 | Andrea Romano als Woman (voice) |
 | Travis Willingham als Morgue Guy / Penguin Thug (voice) |
 | Mick Wingert als Joker Security Guy (voice) |
| Ethan Spaulding | Director |
| Jay Oliva | Director |
| Benjamin Melniker | Executive Producer |
| Sam Register | Executive Producer |
| Heath Corson | Writer |
| Michael Uslan | Executive Producer |
| James Tucker | Supervising Producer |
| Robert J. Kral | Original Music Composer |
| Christopher D. Lozinski | Editor |
| Sam Liu | Storyboard Designer |
| Alan Burnett | Co-Producer |
| Bob Kane | Characters |
| Lew Schwartz | Characters |
| John Ostrander | Characters |
| John Byrne | Characters |
| Paul Dini | Characters |
| Bruce Timm | Characters |
| Sunisa Petchpoo | Production Manager |
| Meredith Layne | Casting Director |
| Andrea Romano | Other |
| Matt Singer | Post-Production Manager |
| Mark A. Keatts | Supervising Dialogue Editor |
| Mark A. Keatts | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Patrick J. Foley | Dialogue Editor |
| Patrick J. Foley | Foley Editor |
| Michael Garcia | Dialogue Editor |
| Michael Garcia | ADR Editor |
| Gabriel Thorburn | Online Editor |
| Christine H. Luethje | Music Editor |
| Niki Sherrod | Other |
| Liz Carroll | Administration |
| Bobbie Page | Production Supervisor |
| Ed Adams | Production Manager |
| Toshiyuki Hiruma | Line Producer |
