 | Zach Braff als Chicken Little (voice) |
 | Garry Marshall als Buck Cluck (voice) |
 | Don Knotts als Mayor Turkey Lurkey (voice) |
 | Amy Sedaris als Foxy Loxy (voice) |
 | Steve Zahn als Runt of the Litter (voice) |
 | Joan Cusack als Abby Mallard (voice) |
 | Patrick Stewart als Mr. Woolensworth (voice) |
 | Fred Willard als Melvin - Alien Dad (voice) |
 | Catherine O'Hara als Tina - Alien Mom (voice) |
 | Wallace Shawn als Principal Fetchit (voice) |
 | Harry Shearer als Dog Announcer (voice) |
 | Mark Walton als Goosey Loosey (voice) |
 | Sean Elmore als Kirby - Alien Kid (voice) |
 | Matthew Josten als Kirby - Alien Kid (voice) |
| Evan Dunn als Kirby - Alien Kid (voice) |
 | Mark Dindal als Morkubine Porcupine / Coach (voice) |
| Dan Molina als Fish Out of Water (voice) |
 | Patrick Warburton als Alien Cop (voice) |
 | Adam West als Ace - Hollywood Chicken Little (voice) |
 | Joe Whyte als Rodriguez / Acorn Mascot / Umpire (voice) |
 | Kelly Hoover als Mama Runt (voice) |
| Will Finn als Hollywood Fish (voice) |
 | Dara McGarry als Hollywood Abby (voice) |
| Mark Kennedy als Hollywood Runt (voice) |
 | Terri Douglas als Additional Voices (voice) |
 | Pat Fraley als Additional Voices (voice) |
 | Scott Menville als Additional Voices (voice) |
| Aaron Spann als Additional Voices (voice) |
 | Nathan Kress als Additional Voices (voice) |
 | Brittney Lee Harvey als Additional Voices (voice) |
 | Chris Edgerly als Additional Voices (voice) |
 | Julianne Buescher als Additional Voices (voice) |
 | Mona Marshall als Additional Voices (voice) |
 | Eddie Frierson als Additional Voices (voice) |
| Mark Dindal | Director |
| John Debney | Original Music Composer |
| Jen Rudin | Casting |
| David Womersley | Production Design |
| Randy Fullmer | Producer |
| Matthew Jon Beck | Casting |
| Ian Gooding | Art Direction |
| Dan Molina | Editor |
| Mark Kennedy | Story |
| Ron Anderson | Screenplay |
| Mark Dindal | Story |
| David Reynolds | Additional Dialogue |
| Ron J. Friedman | Screenplay |
| Steve Bencich | Screenplay |
| Sandra Tsing Loh | Additional Dialogue |
| Dan Cooper | Co-Art Director |
| Kevin Geiger | CG Supervisor |
| Kyle Odermatt | CG Supervisor |
| Eric Powers | Technical Supervisor |
| Thomas Baker | Layout |
| Rick Moore | Layout |
| Leonard Robledo | Visual Development |
| Matsune Suzuki | Visual Development |
| Dan Read | Visual Development |
| Hi-Hat | Choreographer |
| Jim Harrison | Music Editor |
| Jeff Carson | Music Editor |
| Earl Ghaffari | Music Editor |
| Colin Eckart | Visual Development |
| Eamonn Butler | Animation Supervisor |
| Adolph Lusinsky | Visual Development |
| Paul Lanum | Production Manager |
| Robert L. Sephton | Supervising Sound Editor |
| Kelly McGraw | Visual Development |
| Mary Hidalgo | Casting |
| Thomas Cardone | Visual Development |
| Gregory Miller | Visual Development |
| Charles Tappan | Visual Development |
| Michelle Lee Robinson | Visual Development |
| Carol C. Hayden | Visual Development |
| Jeffrey R. Ranjo | Visual Development |
| Robert L. Sephton | Sound Designer |
| Umakanth Thumrugoti | Visual Development |
| Alessandro Jacomini | Visual Development |
| Karen de Jong | Visual Development |
| Natalie Franscioni-Karp | Visual Development |
| Kevin Nelson | Visual Development |
| Peter Del Vecho | Associate Producer |
| Steve Goldberg | Visual Effects Supervisor |
| Kyle Strawitz | Visual Development |
| Sean Eckols | Visual Development |
| Daniel Hu | Layout |
| Robert Neuman | Layout |
| Doug Walker | Layout |
| Liza Breuninger | Production Accountant |
| Scott Beattie | Layout |
| William Otsuka | Layout |
| Adam Kopald | Sound Effects Editor |
| Christian P. Minkler | Sound Re-Recording Mixer |
| Chris DeLaGuardia | Color Timer |
| Allen Blaisdell | Layout |
| Jeff Sawyer | Sound Effects Editor |
| David E. Fluhr | Sound Re-Recording Mixer |
| Mark Henley | Layout |
| Brian Kesinger | Layout |
| David Reynolds | Other |
| Jin Kim | Animation |
| Vera Pacheco | Animation |
| Jason Ryan | Supervising Animator |
| Nik Ranieri | Supervising Animator |
| Mark Anthony Austin | Supervising Animator |
| Dick Zondag | Supervising Animator |
| Doug Bennett | Supervising Animator |
| Joe Grant | In Memory Of |
| Tony Smeed | Supervising Animator |
| Mark Kennedy | Head of Story |
| Terry Moews | Layout Supervisor |
