 | Adam Devine als Ed (voice) |
 | Rachel Bloom als Op (voice) |
 | Zazie Beetz als Dottie (voice) |
 | Ken Jeong als Clarance (voice) |
 | Jim Jefferies als Burnie (voice) |
 | Catherine O'Hara als Alma (voice) |
 | Reggie Watts als Hoss / Ernest Shackleton (voice) |
 | Henry Winkler als Jepson (voice) |
 | Alex Borstein als Mali (voice) |
 | Benedict Wong als Dr. Chung (voice) |
 | Julia Chan als Mrs. Sun / Little Girl (voice) |
 | Tom Hollander als Charles Darwin (voice) |
 | Richard Kind als Wally (voice) |
 | Steve Aoki als Vinny (voice) |
 | Nick Frost als Captain (voice) |
 | Jon Lovitz als Conch (voice) |
 | Sydney Liu als Bo (voice) |
| Maria Bamford als Bo's Mom (voice) |
 | Raymond S. Persi als That Guy / Booby (voice) |
 | Terry Gross als Bomb (voice) |
 | David Silverman als Cyclops / Donut Store Clerk (voice) |
 | Kelly Hu als News Reporter (voice) |
 | Tom Kenny als Mozart / Sled Dogs (voice) |
 | Jason Hightower als Narrator / Sled Dogs (voice) |
 | Kevin Michael Richardson als Sled Dogs (voice) |
| Brenden P. Hill als Baseball Announcer (voice) |
| Wilhelm Boyens als Additional Voices |
| Rob LaZebnik | Writer |
| Raymond S. Persi | Co-Director |
| Joel Cohen | Writer |
| John Frink | Writer |
| David Silverman | Director |
| Evgeni Tomov | Production Design |
| Ryan O'Loughlin | Character Designer |
| Steven Liu | Editor |
| Linda LaMontagne | Casting |
| James Woodham | Additional Writing |
| Matthew Berkowitz | Producer |
| Yanming Jiang | Producer |
| Wang Zhonglei | Producer |
| Joe M. Aguilar | Producer |
| Bo An | Executive Producer |
| Markus Manninen | Executive Producer |
| Raymond Hau | Executive Producer |
| Noah Segal | Executive Producer |
| Jay Stern | Executive Producer |
| Aaron Dem | Executive Producer |
| Markus Manninen | Senior Visual Effects Supervisor |
| Brenden P. Hill | Co-Executive Producer |
| Helen Jiang | Co-Executive Producer |
| Jiawen Wu | Co-Executive Producer |
| Helen Marie Saric | Associate Producer |
| Yilin Li | Associate Producer |
| Shiyuan Peng | Associate Producer |
| Simon Wang | Associate Producer |
| Joel Cohen | Producer |
| John Frink | Producer |
| Rob LaZebnik | Producer |
| Matt Gossen | Additional Writing |
| Kennedy Tarrell | Story Artist |
| Patrick Mate | Story Artist |
| Quinne Larsen | Story Artist |
| Bill Riling | Story Artist |
| Shannon O'Connor | Story Artist |
| Steve Garcia | Story Artist |
| Sandra Fox | Foley Artist |
| Goro Koyama | Foley Artist |
| Simon Rodgers | Visual Development |
| Jonathan Hoekstra | Visual Development |
| Francisco Mora | Visual Development |
| Natalie Franscioni-Karp | Visual Development |
| Vahe Yefremian | Visual Development |
| Kristina Vardazaryan | Visual Development |
| Zac Retz | Visual Development |
| Natasha Wicke | Story Artist |
| Pierre-Alexandre Comtois | Story Artist |
| Amy Mai | Story Artist |
| Ricardo Osuna | Story Artist |
| Kimberly Jo Mills | Story Artist |
| Cory Toomey | Story Artist |
| Mick Giacchino | Original Music Composer |
| Michael Giacchino | Original Music Composer |
| Jason Ryan | Animation Director |
| Alaa Afifeh | Lead Animator |
| Nadine Lavoie | Lead Animator |
| Jonathan Philippon | Lead Animator |
| Gabriel Bégin | Lead Animator |
| Prasanjib Nag | Lead Animator |
| Vincent Reumont | Lead Animator |
| Jack Heeren | Foley Mixer |
| Kevin Schultz | Foley Mixer |
| Don White | Foley Mixer |
| Davi Aquino | Foley Recordist |
| Jenna Dalla Riva | Foley Recordist |
| Chelsea Body | Foley Recordist |
| Curtis Colton Green | Orchestrator |
| Chris Kazmier | Visual Effects Supervisor |
| Damien Simonklein | Producer |
| Leiki Veskimets | Production Manager |
| Kevin Reed | Story Artist |
| Peter DeSève | Character Designer |
