 | Robert Downey Jr. als Dr. John Dolittle |
 | Antonio Banderas als King Rassouli |
 | Michael Sheen als Dr. Blair Müdfly |
 | Jim Broadbent als Lord Thomas Badgley |
 | Jessie Buckley als Queen Victoria |
 | Harry Collett als Tommy Stubbins |
 | Emma Thompson als Poly (voice) |
 | Rami Malek als Chee-Chee (voice) |
 | John Cena als Yoshi (voice) |
 | Kumail Nanjiani als Plimpton (voice) |
 | Octavia Spencer als Dab-Dab (voice) |
 | Tom Holland als Jip (voice) |
 | Craig Robinson als Kevin (voice) |
 | Ralph Fiennes als Barry (voice) |
 | Selena Gomez als Betsy (voice) |
 | Marion Cotillard als Tutu (voice) |
 | Kasia Smutniak als Lily Dolittle |
 | Carmel Laniado als Lady Rose |
 | Frances de la Tour als Dragon (voice) |
 | Jason Mantzoukas als James the Dragonfly (voice) |
 | Ralph Ineson als Arnall Stubbins |
 | Joanna Page als Bethan Stubbins |
 | Sonny Ashbourne Serkis als Arnall Stubbins Jr. |
 | Oliver Chris als Sir Gareth |
 | Clive Francis als Archbishop |
 | Paul Holowaty als Navy Man |
 | Elliot Barnes-Worrell als Captain William Derrick |
 | Mark Umbers als Lieutenant |
 | David Sheinkopf als Don Carpenterino |
 | Sid Sagar als Jeff the Prisoner |
 | Martin Pemberton als Postman |
 | Tim Treloar als Humphrey the Whale (voice) |
| Jim Carretta als Beard Mouse and Leona (voice) |
 | Nick Fisher als Mini the Sugar Glider (voice) |
 | Matt King als Clyde |
 | Ranjani Brow als Mouse (voice) |
 | Kelly Stables als Mouse (voice) |
 | Scott Menville als Army Ant (voice) |
 | Gia Davis als Baby Animal (voice) |
| Henry Holcomb als Baby Animal (voice) |
 | Kyrie McAlpin als Baby Animal (voice) |
 | Isley Zamora als Baby Animal (voice) |
 | Stewart Scudamore als Pirate |
 | Samson Kayo als Pirate |
 | John-Luke Roberts als Pirate / Animal Performer |
 | Joseph Balderrama als Prisoner |
 | Daniel Hoffmann-Gill als Prisoner |
 | Jane Leaney als Animal Performer |
 | Josh Jefferies als On Set Chee-Chee / Animal Performer |
 | Shaun McKee als On Set Yoshi / Animal Performer |
 | Richard Soames als Animal Performer |
 | Will Arnett als Rabbit (voice) (uncredited) |
| Stephen Gaghan | Director |
| Joe Roth | Producer |
| Jeff Kirschenbaum | Producer |
| Susan Downey | Producer |
| Stephen Gaghan | Script |
| Hugh Lofting | Character Designer |
| Sarah Bradshaw | Associate Producer |
| Jon Mone | Executive Producer |
| Craig Alpert | Editor |
| Lucy Bevan | Casting |
| Dominic Watkins | Production Design |
| Matthew Gray | Supervising Art Director |
| Oliver Roberts | Art Direction |
| Gary Tomkins | Art Direction |
| Jenny Beavan | Costume Design |
| Zoe Tahir | Hairstylist |
| Frank Cuomo | Production Manager |
| William M. Connor | First Assistant Director |
| Tom Rye | Second Assistant Director |
| Teresa Orlando | Third Assistant Director |
| Tim Browning | Conceptual Design |
| Alison Clements | Draughtsman |
| Chris Munro | Sound Mixer |
| Darren Shearwood | Special Effects |
| Bryce Nielsen | Visual Effects Producer |
| James Bomalick | Stunts |
| Julian Bucknall | First Assistant Camera |
| Alex Bender | Second Unit Director of Photography |
| Ross Grimshaw | Lighting Technician |
| Jay Maidment | Still Photographer |
| Emily Brockmann | Casting Associate |
| Duncan Broadfoot | Location Manager |
| Dew John | Transportation Captain |
| Jimmy Rich | Assistant Set Dresser |
| Guillermo Navarro | Director of Photography |
| Danny Elfman | Music |
| Will Coubrough | Art Direction |
| Peter Dorme | Art Direction |
| Tom Weaving | Art Direction |
| Tom Whitehead | Art Direction |
| Joseph Koniak | Hair Setup |
| Sebastian Lochmann | Concept Artist |
| Chris Lyons | Special Effects Makeup Artist |
| Frank Cuomo | Post Production Supervisor |
| Simone Goodridge | Unit Production Manager |
| Mark Layton | Production Supervisor |
| Richard Ruck | Unit Manager |
| Joey Coughlin | Second Assistant Director |
| Simon Crane | Second Unit Director |
| Mark Johnstone | Third Assistant Director |
| Sandrine Loisy | Crowd Assistant Director |
| Marlon Beyer Rieger | Third Assistant Director |
| Stephen Gaghan | Screenplay |
| Thomas Shepherd | Screenplay |
| Hugh Lofting | Characters |
| David Allcock | Storyboard Artist |
| Dan Gregor | Screenplay |
| Doug Mand | Screenplay |
| Alexander T.H. Browne | VFX Artist |
| Prakash Goyal | VFX Artist |
| Ramon Álvarez | Stunts |
| Natalie Wood | Stunts |
| Donna Williams | Stunts |
| Martin Wilde | Stunts |
| Billie Jo White | Stunts |
| Richard Wheeldon | Stunts |
| Haydn Webb | Stunts |
| Andy Wareham | Stunts |
| Chris Waite | Stunts |
| Mark Aaron Wagner | Stunts |
| Ivo Vonbank | Stunts |
| Christian Arranz Villareal | Stunts |
| Pablo Verdejo | Stunts |
| Edward Upcott | Stunt Double |
| Edward Upcott | Stunts |
| Evie Twyford | Stunts |
| Anoushka Turner | Stunts |
| Arran Topham | Stunts |
| Mens-Sana Tamakloe | Stunts |
| John Street | Stunts |
| Matthew Stirling | Stunts |
| Anna Stephenson | Stunts |
| Sam Stefan | Stunts |
| Ian Songhurst | Stunts |
| Graeme Smiles | Stunts |
| Anton Simpson-Tidy | Stunts |
| Matt Sherren | Stunts |
| Stephen W. Schriver | Stunt Double |
| Hasit Savani | Stunts |
| Allyson Ryan | Stunts |
| Douglas Robson | Stunts |
| Zach Roberts | Stunts |
| Eden Reyes | Stunts |
| Mark Rayner | Stunts |
| Tilly Powell | Stunts |
| Chris Pollard | Stunts |
| Oleg Podobin | Stunts |
| Martin Pemberton | Stunts |
| Rob Pavey | Stunt Double |
| Rob Pavey | Stunts |
| James Pavey | Stunts |
| Pete Olivant | Stunts |
| Benos Noble | Stunts |
| Brian Nickels | Stunts |
| Elliot Murray | Stunts |
| Steven Mullins | Stunts |
| Erol Mehmet | Stunts |
| Sonny Louis | Stunts |
| Sarah Lochlan | Stunts |
| Richard Leggett | Stunts |
| André Layne | Stunts |
| Andrew Layne | Stunts |
| Monty Lathrope | Stunts |
| Bogdan Kumšackij | Stunts |
| George Kirby | Stunts |
| Joe Kennard | Stunts |
| Steve Jehu | Stunts |
| Rob Hunt | Stunts |
| Michael Holly | Stunts |
| Lyndon S. Hellewell | Stunts |
| George Hayes | Stunts |
| Elliot Hawkes | Stunts |
| Tom Hatt | Stunts |
| Richard Hansen | Stunts |
| Richard Hall | Stunts |
| Franki Hackett | Stunts |
| Nic Goodey | Stunts |
| Angel Luis Gomea | Stunts |
| Paul Ginns | Stunts |
| David Garrick | Stunts |
| Glenn Foster | Stunts |
| Mark Epstein | Stunts |
| Andrew Eadie | Stunts |
| Harley Durst | Stunts |
| Levan Doran | Stunts |
| Will Dent | Stunts |
| Will Dent | Utility Stunts |
| Steve Dent | Stunts |
| Sam Dent | Stunts |
| Maria Dent | Stunts |
| Kelly Dent | Stunts |
| John Dent | Stunts |
| Charlotte Dent | Stunts |
| Bobby Dent | Stunts |
| Shay Dempsey | Stunts |
| Andrius Davidenas | Stunts |
| Simon Crane | Stunt Coordinator |
| Rob Cooper | Stunts |
| Joel Conlan | Stunts |
| Adam Collins | Stunts |
| Carlos Castillo | Stunts |
| Marvin Campbell | Stunts |
| Dacio Caballero | Stunts |
| Katy Bullock | Stunts |
| Grace Bruce | Stunt Double |
| Grace Bruce | Stunts |
| Fred Brown | Stunts |
| Scott Brady | Stunt Double |
| Alexander Bracq | Stunts |
| Rachelle Biernat | Stunts |
| Rachelle Beinart | Stunt Double |
| Lloyd Bass | Stunts |
| Callum Barnes | Stunts |
| Russell Balogh | Stunt Double |
| Russell Balogh | Stunts |
| Daniel Awde | Stunts |
| Benjamin Ashley | Stunts |
| Gary Arthurs | Stunts |
| Luis Miguel Arranz | Stunts |
| Luis Arranz Villareal | Stunts |
| Luis Miguel Arranz Losa | Stunts |
| Eugenio Alonso Yenes | Stunts |
| Matt Crook | Stunts |
| Nina Jalava | Stand In |
| Matt Haggerty | Second Assistant Director |
| John Flemming | Key Grip |
| Jack Flemming | Dolly Grip |
| Steve Kitchen | Rigging Gaffer |
| David Lee | Gaffer |
| Dani Rose | Drone Pilot |
| Pat Sweeney | Gaffer |
| Bill Abbott | Music Editor |
| Pete Anthony | Orchestrator |
| Steve Bartek | Orchestrator |
| Oliver Hug | Music Editor |
| Stephanie Lowry | Music Editor |
| Edgardo Simone | Orchestrator |
| Graham Sutton | Music Editor |
| Edward Trybek | Orchestrator |
| Jo Beckett | Script Supervisor |
| James Madejski | Script Coordinator |
| Sarah Bradshaw | Unit Production Manager |
| William M. Connor | Co-Producer |
| Peter James | Standby Art Director |
| Andrew Proctor | Assistant Art Director |
| Matt Robinson | Assistant Art Director |
| Aisha Saeed | Art Department Coordinator |
| Lee Sandales | Set Decoration |
| Gary Spratling | "A" Camera Operator |
| Dave Hamilton-Green | "B" Camera Operator |
| Malcolm McLean | "C" Camera Operator |
| Julian Bucknall | First Assistant "A" Camera |
| David Pearce | Second Assistant "A" Camera |
| Robert Palmer | First Assistant "B" Camera |
| Alex Bender | Second Assistant "B" Camera |
| James Leckey | First Assistant "C" Camera |
| Morgan Spencer | Second Assistant "C" Camera |
| Alex Collings | Camera Loader |
| Giles Taylor | Camera Trainee |
| Francesco Giardiello | Digital Imaging Technician |
| David Cheesman | Property Master |
| Laura Dishington | Graphic Designer |
| Natasha Jones | Graphic Designer |
| Chris Munro | Production Sound Mixer |
| Martin Conway | Best Boy Electric |
| Derek Russell | Best Boy Grip |
| Brett Flemming | Dolly Grip |
| Dave Armstrong | Dolly Grip |
| Tracy McGregor | Assistant Costume Designer |
| India Witzand | Assistant Costume Designer |
| David Otzen | Costumer |
| Marco Scotti | Costume Supervisor |
| Sheara Abrahams | Costume Set Supervisor |
| Oliver Southall | Costumer |
| Emily Thomson | Costumer |
| Marco De Luca | Costumer |
| Janine Cunliffe | Costumer |
| Celia Yau | Costumer |
| Adam Redmore | Costumer |
| Cédric Andries | Costume Coordinator |
| Daniel Phillips | Makeup Designer |
| Daniel Phillips | Hair Designer |
| Pippa Woods | Makeup Supervisor |
| Pippa Woods | Hair Supervisor |
| Paula Price | Makeup Artist |
| Loulia Sheppard | Makeup & Hair |
| Andrea Cracknell | Makeup & Hair |
| Rachel Korn | Associate Producer |
| Michael Mann | Production Coordinator |
| Edward Squires | Assistant Production Coordinator |
| Kerryn Clements | Assistant Production Coordinator |
| Laurence Cooper | Production Secretary |
| Bill Darby | Location Manager |
| Tom Crooke | Location Manager |
| Ruby Whitelocke | Assistant Location Manager |
| Harry Le Page | Assistant Location Manager |
| Dan Peacock | Assistant Location Manager |
| Sam Turner | Assistant Location Manager |
| Harriet Wharton | Location Coordinator |
| Ed Burrell | Location Assistant |
| Chris Cranstoun | Location Assistant |
| Claire Robertson | Production Accountant |
| Giles Barnett | First Assistant Accountant |
| Karen Bicknell | Payroll Accountant |
| Neil Pierson | Payroll Accountant |
| Charles Larcombe | Assistant Accountant |
| Laura Anderson | Assistant Accountant |
| Claire Treacy | Assistant Accountant |
| Olivia Grant | Casting Assistant |
| Brian Neighbour | Construction Manager |
| Usha Chaman | Construction Coordinator |
| Stacy Mann | Unit Publicist |
| Lisa Rodgers | Post Production Supervisor |
| Jim Carretta | Additional Editor |
| Jon Taylor | Sound Re-Recording Mixer |
| Frank A. Montaño | Sound Re-Recording Mixer |
| Julian Andraus | First Assistant Editor |
| Brandon Cano-Errecart | Assistant Editor |
| Daniel Laurie | Supervising Sound Editor |
| J.R. Grubbs | Supervising Sound Editor |
| Al Nelson | Sound Designer |
| Benjamin A. Burtt | Sound Effects Editor |
| Qianbaihui Yang | Sound Effects Editor |
| Jack Whittaker | Sound Effects Editor |
| Jacob Riehle | Dialogue Editor |
| Brad Semenoff | Dialogue Editor |
| Luke Dunn Gielmuda | Foley Supervisor |
| Andre Fenley | Foley Editor |
| Alyssa Nevarez | Assistant Sound Editor |
| Jana Vance | Foley Artist |
| Ronni Brown | Foley Artist |
| Jeff Gomillion | ADR Mixer |
| Nick Roberts | ADR Mixer |
| Chris Lebenzon | Editor |
| Nick Moore | Additional Editing |
| Fraser Taggart | Second Unit Director of Photography |
| Martin Bell | Pre-Visualization Supervisor |
