 | Sean Connery als James Bond |
 | Ursula Andress als Honey Ryder |
 | Joseph Wiseman als Dr. No |
 | Jack Lord als Felix Leiter |
 | Anthony Dawson als Professor R. J. Dent |
 | Zena Marshall als Miss Taro |
 | John Kitzmiller als Quarrel |
 | Eunice Gayson als Sylvia Trench |
 | Bernard Lee als M |
 | Lois Maxwell als Miss Moneypenny |
 | Peter Burton als Major Boothroyd |
 | Yvonne Shima als Sister Lily |
 | Michel Mok als Sister Rose |
 | Marguerite LeWars als Annabel Chung - Photographer |
 | William Foster-Davis als Superintendent Duff |
 | Dolores Keator als Mary Trueblood |
 | Reginald Carter als Mr. Jones |
 | Louis Blaazer als Pleydell-Smith |
 | Colonel Burton als General Potter |
 | John Hatton als Signals Foreman (uncredited) |
| Byron Lee als Bandleader (uncredited) |
 | Timothy Napier Moxon als Professor John Strangways (uncredited) |
 | Malou Pantera als Hotel Receptionist (uncredited) |
 | Lester Prendergast als Puss Feller (uncredited) |
 | Diana Coupland als Honey Ryder - Singing Voice (voice) (uncredited) |
 | Nikki Van der Zyl als Honey Ryder / Sylvia Trench / Various (voice) (uncredited) |
| Adrian Robinson als Hearse Driver |
| Ian Fleming | Novel |
| Richard Maibaum | Screenplay |
| Ted Moore | Director of Photography |
| Syd Cain | Art Direction |
| Terence Young | Director |
| Harry Saltzman | Producer |
| Monty Norman | Original Music Composer |
| Johanna Harwood | Screenplay |
| Albert R. Broccoli | Producer |
| Peter R. Hunt | Editor |
| Ken Adam | Production Design |
| Berkely Mather | Screenplay |
| John Shinerock | Focus Puller |
| Maureen Whitty | Production Secretary |
| John O'Gorman | Makeup Artist |
| Eileen Warwick | Hairstylist |
| L. C. Rudkin | Production Manager |
| Clive Reed | Assistant Director |
| Freda Pearson | Set Dresser |
| John Dennis | Sound Recordist |
| Wally Milner | Sound Recordist |
| Frank George | Special Effects |
| Jimmy Spoard | Grip |
| John Winbolt | Camera Operator |
| Trevor Bond | Animation |
| Tessa Prendergast | Costumer |
| Ben Rayner | Assistant Editor |
| John Barry | Music Arranger |
| Burt Rhodes | Orchestrator |
| Eric Rogers | Conductor |
| Helen Whitson | Continuity |
| Maurice Binder | Main Title Designer |
| Michael Borota | Stand In |
| Albert R. Broccoli | Presenter |
| Harry Saltzman | Presenter |
| Laurie Shane | Electrician |
| Bunny Yeager | Still Photographer |
| Stanley Sopel | Associate Producer |
| James Liggat | Casting |
| Bert Cann | Still Photographer |
| Eileen Sullivan | Wardrobe Master |
| John Brady | Wardrobe Master |
| Chris Blackwell | Location Manager |
