 | Ethan Hawke als Vincent Freeman |
 | Uma Thurman als Irene Cassini |
 | Jude Law als Jerome Eugene Morrow |
 | Alan Arkin als Detective Hugo |
 | Loren Dean als Adult Anton Freeman |
 | Gore Vidal als Director Josef |
 | Tony Shalhoub als "German" |
 | Ernest Borgnine als Caesar |
 | Blair Underwood als Geneticist |
 | Xander Berkeley als Dr. Lamar |
 | Jayne Brook als Marie Freeman |
 | Una Damon als Head Nurse |
 | Elias Koteas als Antonio Freeman |
 | Maya Rudolph als Delivery Nurse |
 | Elizabeth Dennehy als Preschool Teacher |
 | Mason Gamble als Younger Vincent |
 | Vincent Nielson als Younger Anton |
| Chad Christ als Young Vincent |
 | William Lee Scott als Young Anton |
| Clarence Graham als Personnel Officer |
| Carlton Benbry als Gattaca Hoover |
 | Susan Jennifer Sullivan als Sequencing Customer |
 | Ken Marino als Sequencing Technician |
 | Cynthia Martells als Cavendish |
 | Gabrielle Reece als Gattaca Trainer |
| Ryan Dorin als Twelve-Fingered Pianist |
 | Dean Norris als Cop on the Beat |
 | Russell Milton als Gattaca Detective |
| George Marshall Ruge als Beaten Detective |
| Steve Bessen als Blood Test Detective |
 | Lindsey Ginter als Mission Commander |
 | Greg Sestero als Gattacan Citizen (uncredited) |
| Lisa Zeno Churgin | Editor |
| Andrew Niccol | Director |
| Danny DeVito | Producer |
| Michael Nyman | Original Music Composer |
| Slawomir Idziak | Director of Photography |
| Colleen Atwood | Costume Design |
| Michael Shamberg | Producer |
| Stacey Sher | Producer |
| Francine Maisler | Casting |
| Stephen Alesch | Set Designer |
| Richard King | Supervising Sound Editor |
| Jan Roelfs | Production Design |
| Gary D'Amico | Special Effects Coordinator |
| Jerry Pooler | Visual Effects Supervisor |
| Chris Watts | Visual Effects Supervisor |
| Darren Michaels | Still Photographer |
| Steven Ticknor | Sound Effects Editor |
| Toni-Ann Walker | Hairstylist |
| R. Scott Doran | Leadman |
| Paul Massey | Sound Re-Recording Mixer |
| Robert Q. Mathews | Costume Supervisor |
| Sarah Knowles | Art Direction |
| Daniele Colajacomo | Visual Effects Supervisor |
| Jill Rockow | Makeup Artist |
| Jim Ondrejko | Construction Coordinator |
| Norman Howell | Stunt Coordinator |
| Jonathan Brown | Camera Operator |
| Randall D. Wilkins | Set Designer |
| Doug Hemphill | Sound Re-Recording Mixer |
| Lizbeth Williamson | Makeup Artist |
| Nancy Nye | Set Decoration |
| Lawrence L. Commans | Boom Operator |
| Gary A. Hecker | Foley Artist |
| Cliff Latimer | ADR Supervisor |
| Emily Ferry | Property Master |
| Scott A. Tinsley | Foley Editor |
| Jeffrey R. Payne | Foley Editor |
| Wilma Garscadden-Gahret | Script Supervisor |
| James Matheny | Dialogue Editor |
| Noah Blough | Dialogue Editor |
| Jeffrey Wilhoit | Foley Artist |
| Cynthia Biamon | Music Coordinator |
| Nerses Gezalyan | Foley Mixer |
| Georgia Kacandes | Unit Production Manager |
| Michael M. Krevitt | Key Grip |
| Matthew Nelson | Second Assistant Camera |
| Helen Hand | Second Film Editor |
| James C. Taylor | Transportation Captain |
| Catherine Calleson | Assistant Sound Editor |
| David Robbins | Additional Music |
| Brian Penikas | Prosthetic Makeup Artist |
| Beau Bonneau | Extras Casting |
| Ilt Jones | Assistant Location Manager |
| Joe Angel Rodriguez | Producer's Assistant |
| Patrick Noonan | Production Assistant |
| David Morton | Rigging Gaffer |
| Don Elliott | Swing |
| Joy Taylor | Assistant Property Master |
| Robert Earl Craft | Location Manager |
| Roger James Ferrin | Production Assistant |
| Cynthia Haagens | Assistant Sound Editor |
| Tim Swan | Unit Production Manager |
| John T. Connor | First Assistant Camera |
| Mo Henry | Negative Cutter |
| Stephan von Hase | Production Sound Mixer |
| Tim Swan | Production Supervisor |
| Daniel Silverberg | Second Assistant Director |
| Andrew Niccol | Writer |
| Pamela Abdy | Producer's Assistant |
| Emma Tillinger Koskoff | Actor's Assistant |
| Michael A. Mendez | Grip |
| Elizabeth Tompkins | Production Accountant |
| Shari LaFranchi Blakney | Production Coordinator |
| Bunny Andrews | Music Editor |
| Otie Brown | Second Assistant Director |
| Michael J. Hogan | Video Assist Operator |
| Steve Dellerson | Visual Effects Coordinator |
| Dondi Bastone | Music Consultant |
| Joshua Levinson | Associate Producer |
| Bradley Cramp | Production Supervisor |
| Tyson Dexter | Stunt Double |
| Brennan Dufresne | Casting Assistant |
| Bob Putynkowski | Color Timer |
| Kathleen Roll | Production Assistant |
| Dick Hansen | Cableman |
| Gail Lyon | Co-Producer |
| Bette Iverson | Key Hair Stylist |
| Jennifer Lax | Key Costumer |
| Maurice Schwartzman | Choreographer |
| Edward Allen | First Assistant Accountant |
| David Jernigan | Transportation Co-Captain |
| Spooky Stevens | Unit Publicist |
| Thomas Wazney | Best Boy Grip |
| John Wayne Randolph | Craft Service |
| Dan Levitan | Compositor |
| Lisa Yesko | Post Production Supervisor |
| Mike Smith | Stunts |
| Melody Beam | Production Assistant |
| Ve Neill | Makeup Supervisor |
| Alicia M. Tripi | Hair Department Head |
| Otniel Gonzalez | Assistant Property Master |
| Michael Cassidy | Stunt Coordinator |
| Robert Jason | Chief Lighting Technician |
| John Spence | First Assistant Editor |
| Janek Sirrs | Visual Effects |
| Georgia Kacandes | Associate Producer |
| John R. Woodward | First Assistant Director |
| Lizzie Friedman | Producer's Assistant |
| Jeff Smith | Dolly Grip |
| Mario West | Production Assistant |
| Kathryn Eisenstein | Casting Associate |
| Evan A. Nesbitt | First Assistant Camera |
| Stephanie Higgins Frey | Production Assistant |
| Michael Riley | Title Designer |
| Ken Dackermann | Digital Compositor |
