 | Frank Welker als Scooby-Doo / Fred Jones (voice) |
 | Grey DeLisle als Daphne Blake (voice) |
 | Matthew Lillard als Shaggy Rogers (voice) |
 | Kate Micucci als Velma Dinkley (voice) |
 | Cassandra Peterson als Elvira, Mistress of the Dark (voice) |
 | Bill Nye als Bill Nye (voice) |
 | Dwight Schultz als Dr. Crane, AKA: Scarecrow (voice) |
 | Kamali Minter als Michelle (Voice) |
 | Bumper Robinson als Mike (Voice) |
 | Roger Craig Smith als Autopilot / Parent (Voice) |
 | Fred Tatasciore als Alpha Jackal-Lantern / Federal Agent Malarkey (Voice) |
 | David Herman als Sheriff, AKA: Cutler Toe (Voice) |
| Maxwell Atoms | Director |
| Maxwell Atoms | Screenplay |
| Maxwell Atoms | Producer |
| Sam Register | Executive Producer |
| Kris Zimmerman | Casting |
| Mike Mangan | Editor |
| Steve Rucker | Original Music Composer |
| Robert J. Kral | Additional Music |
| Shaun Avnet | Line Producer |
| Colin A.B.V. Lewis | Line Producer |
| Monique Mosbe | Assistant Production Manager |
| Daniel Schier | Character Designer |
| Damon Moran | Prop Designer |
| Demetrice Wilson | Prop Designer |
| Joey Adams | Storyboard Artist |
| Tim Divar | Storyboard Artist |
| Danny Ducker | Storyboard Artist |
| Greg Emerson | Other |
| Brittany Canasi | Post-Production Manager |
| Jeff O. Collins | Other |
| Mark A. Keatts | Supervising Dialogue Editor |
| Mark A. Keatts | ADR Editor |
| David M. Cowan | Dialogue Editor |
| David M. Cowan | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Patrick J. Foley | Dialogue Editor |
| Patrick J. Foley | ADR Editor |
| Michael Garcia | Dialogue Editor |
| Michael Garcia | ADR Editor |
| David Cowles | Online Editor |
| Cris Mertens | Other |
| Frances E. Chang | Administration |
| Liz Carroll | Administration |
| Debbie Lindquist | Production Accountant |
| Chaz Murphy | Production Accountant |
| Maral Simonian | Production Accountant |
| Athena Wingate | Production Accountant |
| Bobbie Page | Production Supervisor |
| Susan Ward | Production Supervisor |
| Ed Adams | Production Manager |
| Jay Bastian | Executive In Charge Of Production |
| Joe Ruby | Thanks |
| Ken Spears | Thanks |
