 | Danny Trejo als Machete |
 | Michelle Rodriguez als Luz |
 | Jessica Alba als Sartana Rivera |
 | Robert De Niro als Senator John McLaughlin |
 | Steven Seagal als Rogelio Torrez |
 | Cheech Marin als Padre Cortez |
 | Lindsay Lohan als April Booth |
 | Electra Avellan als Nurse Mona |
 | Elise Avellan als Nurse Lisa |
 | Tom Savini als Osiris Amanpour |
 | Don Johnson als Von Jackson |
 | Billy Blair als Von's Henchman |
 | Jeff Fahey als Michael Booth |
 | Shea Whigham als Sniper |
 | Daryl Sabara als Julio |
 | Tito Larriva als Culebra Cruzado |
 | Gilbert Trejo als Jorge |
| Felix Sabates als Doc Felix |
| Alicia Rachel Marek als June |
| Marci Madison als Nurse Fine |
| Tina Rodriguez als Tristana |
 | Stacy Keach als Doc Franklin |
 | James Parks als Edgar McGraw |
 | Ara Celi als Reporter |
 | Mayra Leal als Chica (Naked Girl) |
| Tommy Nix als Dishwasher |
| Alejandro Antonio als Chief |
 | Juan Gabriel Pareja als Rico |
| Roland Ruiz als Luis |
 | Nina Leon als Machete's Wife |
 | Jason Douglas als Patrolman #1 |
 | Mitchell Lance Adams als Patrolman 2 |
| Vic Trevino als Federale Officer |
 | Jim Henry als Guard |
| Nina Proctor | Costume Design |
| Mary Vernieu | Casting |
| Myles Nestel | Executive Producer |
| Rebecca Rodriguez | Editor |
| Robert Rodriguez | Director |
| Álvaro Rodríguez | Screenplay |
| Rick Schwartz | Producer |
| Ed Borgerding | Executive Producer |
| Chingon | Original Music Composer |
| Ashok Amritraj | Executive Producer |
| Alan Bernon | Executive Producer |
| J.C. Cantu | Casting |
| Robert Rodriguez | Screenplay |
| Elizabeth Avellan | Producer |
| Jimmy Lindsey | Director of Photography |
| Robert Rodriguez | Editor |
| Ethan Maniquis | Director |
| Robert Rodriguez | Producer |
| Pete Stockton | Key Rigging Grip |
| Bart Brown | Set Decoration |
| Everett Byrom III | Special Effects Supervisor |
| P.K. Munson | First Assistant "B" Camera |
| Raul Mello | Rigging Gaffer |
| Debbie Cortez Haber | Art Department Coordinator |
| Richard M. Moore | Construction Coordinator |
| Jonathan Shaffer | Property Master |
| Jason McKnight | Electrician |
| Sebastian Vega | First Assistant "A" Camera |
| Ferrell A. Shinnick | Key Grip |
| Rico Torres | Still Photographer |
| Carlos Benavides | Electrician |
| Blake Clifton | Second Assistant "B" Camera |
| Joe Rivera | Hair Department Head |
| Joe McCusker | Construction Coordinator |
| Matt Harshbarger | Second Assistant "A" Camera |
| Matt Fliehler | Electrician |
| Christopher Stull | Production Design |
| Vance Holmes | Camera Operator |
| Wes Dixon | Electrician |
| Joe Vasquez | Best Boy Grip |
| Scott A. Reeder | Property Master |
| Sean Maxwell | Camera Operator |
| Scott Conn | Electrician |
| Todd Smiley | Gaffer |
| Ermahn Ospina | Makeup Department Head |
| Robert Janecka | Best Boy Electric |
| Neil Fraser | Dolly Grip |
| Cheryl Wheeler Duncan | Stunts |
| Russell Towery | Stunt Coordinator |
| Mark Chavarria | Stunts |
| Christopher M. Campos | Stunts |
| Joseph T. Campos | Stunts |
| Jeremy Fitzgerald | Stunts |
| Mitchell Lance Adams | Stunts |
| Troy Robinson | Stunts |
| Mark Norby | Stunts |
