 | Saoirse Ronan als Mary Stuart |
 | Margot Robbie als Queen Elizabeth I |
 | Jack Lowden als Henry Darnley |
 | Joe Alwyn als Robert Dudley |
 | David Tennant als John Knox |
 | Guy Pearce als William Cecil |
 | Gemma Chan als Bess of Hardwick |
 | Martin Compston als Earl of Bothwell |
 | Ismael Cruz Córdova als David Rizzio |
 | Brendan Coyle als Earl of Lennox |
 | Ian Hart als Lord Maitland |
 | James McArdle als James, Earl of Moray |
 | Richard Cant als Thomas Andrews |
 | Guy Rhys als Earl of Kent |
 | Thom Petty als Earl of Shrewsbury |
 | Izuka Hoyle als Mary Seton |
 | John Ramm als Bull |
 | Simon Russell Beale als Robert Beale |
 | Maria Dragus als Mary Fleming |
 | Liah O'Prey als Mary Livingston |
 | Eileen O'Higgins als Mary Beaton |
 | Greg Miller Burns als Thomas Hepburn |
 | Aneurin Pascoe als John Hepburn |
 | Adrian Derrick-Palmer als George Dalgleish |
 | Kal Sabir als The Laird of Ramorny |
 | Luke Kidd als James Haliburton |
 | Adrian Lester als Lord Randolph |
 | Georgia Burnell als Kate Carey |
 | Alan Turkington als Thomas Jenye |
 | Euan Macnaughton als William Kirkcaldy of Grange |
 | Scot Greenan als Hector Maclean |
 | Karen Dunbar als Commoner |
 | Ian Hallard als Sir Richard Knightley |
 | Andrew Rothney als King James I |
 | Angela Bain als Snuffer Woman |
 | Abby Cassidy als Fotheringhay Gentlewoman |
 | Shalisha James-Davis als Fotheringhay Gentlewoman |
 | Adam Bond als Sir William Douglas |
 | Katharine O'Donnelly als Young Holyrood Servant |
 | Grace Molony als Dorothy Stafford |
 | Luke Hobson als Henry Killigrew |
 | Ben Wiggins als John Tamworth |
 | Eldredd Wolf als William Killigrew |
 | Eric MacLennan als Portrait Painter |
 | Nathen East als Andrew Ker of Fawdonside |
 | Daniel Booroff als Knox's Secretary |
 | Sean Buchanan als Francis Yaxley |
 | Matthew Pidgeon als Wedding Announcer |
 | Benjamin Barraclough als Officer |
 | Jordan Turk als James MacDonald |
 | Adam Stevenson als Urie Campbell |
| Scot Greenan als Hector MacLean |
 | Chris Grahamson als Moray's Drummer |
 | Ed Jones als William Taylor |
 | Owen Gorman als Bishop |
 | John Stahl als Fisherman |
 | Kadiff Kirwan als Attaché |
 | Alex Beckett als Sir Walter Mildmay |
 | Will Rowlands als Soldier |
| Josie Rourke | Director |
| John Mathieson | Director of Photography |
| Beau Willimon | Screenplay |
| Alexandra Byrne | Costume Design |
| Chris Dickens | Editor |
| James Merifield | Production Design |
| John Guy | Book |
| Eric Fellner | Producer |
| Tim Bevan | Producer |
| Debra Hayward | Producer |
| Jane Robertson | Co-Producer |
| Ian Wilson | Supervising Sound Editor |
| Max Richter | Original Music Composer |
| Alastair Sirkett | Sound Mixer |
| Gina Cromwell | Set Decoration |
| Richard May | Production Manager |
| Alex Oakley | First Assistant Director |
| Mark Flanagan | Stunts |
| Jenny Shircore | Makeup Designer |
| Jessica Brooks | Makeup Artist |
| Marc Pilcher | Hairstylist |
| Kirsten Lane | Music Consultant |
| Peter Dillon | Stunts |
| Amelia Granger | Executive Producer |
| Liza Chasin | Executive Producer |
| Kate Pakenham | Executive Producer |
| Jenny Shircore | Hair Designer |
| Alastair Coomer | Casting Director |
| Alex Beckett | In Memory Of |
| Russell Balogh | Stunts |
| Tony Condren | Stunt Coordinator |
| Richard Ryan | Stunt Coordinator |
| Jason Beeston | Stunts |
| Adam Behan | Stunts |
| Gerry Behan | Stunts |
| James Bewley | Stunts |
| Sean Bingham | Stunts |
| Lemuel Black | Stunts |
| Dave Boyle | Stunts |
| Aran Condren | Stunts |
| Sean Condren | Stunts |
| Max Cormac | Stunts |
| James Cosgrave | Stunts |
| Darren Costello | Stunts |
| Gavin Coventry | Stunts |
| Ken Doherty | Stunts |
| Shay Doherty | Stunts |
| Levan Doran | Stunts |
| Stav Dvorkin | Stunts |
| Jonathan East | Stunts |
| Stuart Frift | Stunts |
| David Garrick | Stunts |
| Michael Hanna | Stunts |
| Richard Leggett | Stunts |
| Edward Linard | Stunts |
| Jinny Lofthouse | Stunts |
| Nathaniel Marten | Stunts |
| Mathew McKay | Stunts |
| Erol Mehmet | Stunts |
| Jack Miles | Stunts |
| Ciaran O'Grady | Stunts |
| Alex Payne | Stunts |
| Justin Pearson | Stunts |
| Andy Pilgrim | Stunts |
| Dan Styles | Stunts |
| Harvey Taylor | Stunts |
| Richie Wilson | Stunts |
| Lauterio Zamparelli | Stunts |
| Harriet Spencer | Associate Producer |
| James Manning | Second Assistant Director |
| Tania Blunden | Post Production Supervisor |
| Angela Moneke | Production Coordinator |
| Pete Cavaciuti | "A" Camera Operator |
| Pete Cavaciuti | Steadicam Operator |
| Simon Hume | First Assistant "A" Camera |
| Simon Dunn | Second Assistant "A" Camera |
| Rodrigo Gutierrez | "B" Camera Operator |
| Lewis Hume | First Assistant "B" Camera |
| Archie Muller | Second Assistant "B" Camera |
| Angus Hudson | "C" Camera Operator |
| Angus Hudson | Second Unit Director of Photography |
| Keith McNamara | First Assistant "C" Camera |
| Calem Trevor | Second Assistant "C" Camera |
| Derek Walker | "D" Camera Operator |
| Tommy Holman | First Assistant "D" Camera |
| Kat Spencer | Second Assistant "D" Camera |
| Filippo Maso | Camera Trainee |
| Nick Poole | Camera Trainee |
| Dafydd Bates | Camera Trainee |
| Laura Sulca | Camera Trainee |
| Alex Golding | Digital Imaging Technician |
| Anthony Dias | Digital Imaging Technician |
| Lucy Ward | Script Supervisor |
| Robert Flanagan | Production Sound Mixer |
| Paul Naughton | Boom Operator |
| James Wakefield | Supervising Art Director |
| Todd Ellis | Standby Art Director |
| Toby Britton | Art Direction |
| Gavin Fitch | Art Direction |
| Oliver Goodier | Art Direction |
| Gavin Dean | Assistant Art Director |
| Isona Rigau | Assistant Art Director |
| Matthew Robinson | Assistant Art Director |
| Denise Ball | Draughtsman |
| Catherine Whiting | Draughtsman |
| Lotta Wolgers | Draughtsman |
| Tom Turner | Draughtsman |
| Andrew Tilhoo | Draughtsman |
| Jenny Hawes | Art Department Coordinator |
| Tamara Catlin-Birch | Art Department Coordinator |
| Marie Boon | Art Department Assistant |
| Ava Skeet | Art Department Assistant |
| Emma MacDevitt | Assistant Set Decoration |
| Samantha Redwood | Assistant Set Decoration |
| Finlay Britton | Storyboard Artist |
| Brendan Handscombe | Costume Supervisor |
| Laura Smith | Assistant Costume Designer |
| Kate Chadderton | Costume Coordinator |
| Sarah Brest | Wardrobe Master |
| Sarah Kelly | Key Hair Stylist |
| Sarah Kelly | Key Makeup Artist |
| Hannah Edwards | Key Hair Stylist |
| Hannah Edwards | Key Makeup Artist |
| Louise Coles | Makeup & Hair |
| Annette Field | Makeup & Hair |
| Susie Redfern | Makeup & Hair |
| Karen Teitge | Makeup & Hair |
| Matt George | Wigmaker |
| Alex Rouse | Wigmaker |
| Terry Jarvis | Wigmaker |
| Pat Karam | Location Manager |
| Mark Walledge | Location Manager |
| Andrew Ryland | Location Manager |
| Ian Hutchinson | Location Manager |
| Chris Johnston | Location Manager |
| Nathan Flaher | Assistant Location Manager |
| Ian Lumsden | Assistant Location Manager |
| Karl King | Unit Manager |
| Debs Richardson | First Assistant Editor |
| Andy Hague | Visual Effects Editor |
| Heidi Freeman | Visual Effects Editor |
| Nina Annan | Assistant Editor |
| Victoria Blackwood | Assistant Editor |
| Paolo Magni | Assistant Editor |
| Alice Syed | Production Coordinator |
| Imogen Turner | Assistant Production Coordinator |
| Sarah Cook | Production Secretary |
| Alan Martin | Gaffer |
| David Appleby | Key Grip |
| Jim Crowther | Best Boy Grip |
| Gary Romaine | Grip |
| Craig Shiels | Grip |
| Neal Champion | Special Effects Supervisor |
| Isabella Odoffin | Casting Associate |
| Eliza Heslop | Casting Assistant |
| Liam Daniel | Still Photographer |
| Wendy Kidd | Unit Publicist |
| Dean Thomas | Transportation Captain |
| Ian Wilson | Sound Designer |
| Steve Single | Dialogue Editor |
| Adele Fletcher | ADR Editor |
| Alastair Sirkett | Sound Designer |
| Hugo Adams | Foley Supervisor |
| Lilly Blazewicz | Foley Editor |
| Glen Gathard | Foley Mixer |
| Peter Burgis | Foley Artist |
| Zoe Freed | Foley Artist |
| Sue Harding | Foley Artist |
| Steve Single | Sound Re-Recording Mixer |
| Andrew Caller | Sound Re-Recording Mixer |
| Michael Connell | Music Editor |
| Dave Foster | Orchestrator |
| Max Richter | Orchestrator |
| Alicia Brockenbrow | Assistant Director |
