 | Charlize Theron als Aileen Wuornos |
 | Christina Ricci als Selby Wall |
 | Bruce Dern als Thomas |
 | Lee Tergesen als Vincent Corey |
 | Annie Corley als Donna |
 | Pruitt Taylor Vince als Gene / Stuttering "John" |
 | Marco St. John als Evan / Undercover "John" |
 | Marc Macaulay als Will / Daddy "John" |
 | Scott Wilson als Horton / Last "John" |
 | Rus Blackwell als Cop |
 | Tim Ware als Chuck |
 | Stephan Jones als Lawyer |
 | Brett Rice als Charles |
 | Kaitlin Riley als Teenage Aileen |
 | Cree Ivey als 7-Year-Old Aileen |
| Catherine Mangan als Justy |
 | Magdalena Manville als Bar Lap Girl |
| T. Robert Pigott als Bartender |
| Romonda Shaver als Employment Agent |
 | Glenn R. Wilder als Restaurant Manager |
| Elaine Stebbins als Wife at Accident |
 | Kane Hodder als Undercover Cop |
 | Christian Stokes als Undercover Cop |
| Lyllian Barcaski als Bar Girl |
| Nonalee Davis als Bar Girl |
 | Bubba Baker als Cubby |
| Al als Himself |
| Cannonball als Himself |
| Chad Vaccarino als Trevor |
| Gene R. Stephenson als Judge |
| Jesse Stern als Skate Rink Attendant |
| Billy Boylan als Police Chief |
 | Jim R. Coleman als Newscaster |
| Chandra Leigh als Cute Teenage Attendant |
| Lori McDonald als Attendant |
| Adam Brown als Attendant |
| Ed Donovan als State Prison Guard (uncredited) |
| Andreas Grosch | Executive Producer |
| Steven Bernstein | Director of Photography |
| Shawn R. McFall | Set Decoration |
| Orvis Rigsby | Art Direction |
| Rhona Meyers | Costume Design |
| Meagan Riley-Grant | Executive Producer |
| Jane Kurson | Editor |
| Clark Peterson | Producer |
| Andreas Schmid | Executive Producer |
| Patty Jenkins | Director |
| Arthur Coburn | Editor |
| Edward T. McAvoy | Production Design |
| BT | Original Music Composer |
| Mark Damon | Producer |
| Brad Wyman | Producer |
| Sammy Lee | Executive Producer |
| Charlize Theron | Producer |
| Donald Kushner | Producer |
| Ferne Cassel | Casting |
| Patty Jenkins | Screenplay |
| Stewart Hall | Executive Producer |
| Howard Paar | Music Supervisor |
| Kane Hodder | Stunt Coordinator |
| Gene Page | Still Photographer |
| Grayson Austin | Steadicam Operator |
| Brian Bulman | Music Editor |
| Kathe Swanson | Key Hair Stylist |
| Stephanie Lowell | Script Supervisor |
| Lou Kleinman | Dialogue Editor |
| Toni G | Makeup Artist |
| Zack Davis | Sound Supervisor |
| Zack Davis | Sound Designer |
| John Dunn | Sound Effects Editor |
| Peter J. Devlin | Production Sound Mixer |
| Marc Fishman | Sound Re-Recording Mixer |
| Tony Lamberti | Sound Re-Recording Mixer |
| Geoffrey G. Rubay | Sound Supervisor |
| Geoffrey G. Rubay | Sound Designer |
| Pamela Lee Incardona | Costume Supervisor |
| Emae Villalobos | Costume Supervisor |
| David Alvarado | Associate Producer |
| Brent Morris | Co-Producer |
| Lee Grimes | Key Makeup Artist |
| Terrie Irwin | Hair Assistant |
| Rodger Jacobs | Assistant Makeup Artist |
| Mary Hedges Lampert | Hair Assistant |
| Jonah Levy | Assistant Makeup Artist |
| Carol Rasheed | Assistant Makeup Artist |
| Yoichi Art Sakamoto | Prosthetics |
| Deborah Brozovich | Additional Hairstylist |
| Jeff Standard | Makeup Artist |
| Arie Bohrer | Production Supervisor |
| Jimmy Li | Production Supervisor |
| Craig W. Richards | Unit Production Manager |
| Michael Toji | Post Production Supervisor |
| Matthew Waldman | Production Supervisor |
| David Allen Cluck | First Assistant Director |
| Guy Livneh | Second Unit Director |
| Jay Ostrowski | Second Assistant Director |
| Tony Taylor | Second Second Assistant Director |
| Derek W. Tow | Second Unit First Assistant Director |
| Eric Baker | Additional Set Dresser |
| John Balling | Scenic Artist |
| Bethany Berry-Weiss | Additional Set Dresser |
| Tammie N. Byers | Additional Set Dresser |
| Cornellius Carroll | Property Master |
| Stitch Crisp | Property Master |
| Michael Edling | Construction Coordinator |
| Michael Hendrick | Swing |
| Bret Kane | Additional Set Dresser |
| Jeff 'J.Mac' McDonald | Additional Set Dresser |
| James Parker | Additional Set Dresser |
| James B. Perdue | Scenic Artist |
| Alan S. Reynolds | Scenic Artist |
| Karen Rigsby | Art Department Coordinator |
| Gene Siegmund | Property Master |
| Mary Jo Snyder | Scenic Artist |
| John Tegethoff | Additional Set Dresser |
| Andrew Wert | Assistant Property Master |
| Dan Wilkerson | On Set Dresser |
| Jim S. Williams | Leadman |
| Douglas Winters | Construction Foreman |
| Rex Christensen | Set Dresser |
| Steven Barkan | Sound Recordist |
| John Bires | Sound Engineer |
| John Cannon | First Assistant Sound Editor |
| Kevin Cerchiai | Boom Operator |
| Scott Clements | Boom Operator |
| Robert Deschaine | ADR Mixer |
| Jeff Glueck | Sound Engineer |
| Carey Milbradt | Foley Editor |
| Carey Milbradt | Foley Mixer |
| Andy Potvin | Dolby Consultant |
| Tami Treadwell | ADR Recordist |
| Karen Vassar Triest | Sound Editor |
| David Young | Sound Engineer |
| Steve Bartkowicz | Sound Re-Recording Assistant |
| Matt Colleran | Sound Recordist |
| Phillip Beck | Special Effects Coordinator |
| Ron Keller | Special Effects Technician |
| Kevin Morrissey | Special Effects Assistant |
| Erik Dehkhoda | VFX Artist |
| Christopher Dusendschon | Visual Effects Supervisor |
| Dennis Fitzsimmons | VFX Artist |
| Elton Garcia | Main Title Designer |
| Mitch Paulson | Digital Colorist |
| Adam Stern | VFX Artist |
| Shanna Lynn | Stunt Double |
| Kiana Politis | Stunts |
| Ben Bardin | Key Grip |
| Michael Bedore | Electrician |
| Jason Bing | Grip |
| Grady Bishop | Camera Car |
| Jon Bowen | Electrician |
| Kevin J. Burroughs | Electrician |
| Stephen Campbell | Additional Photography |
| Stephen Campbell | Camera Operator |
| Michael 'Doc' Cetrulo | Grip |
| Gary H. Clay | Grip |
| Vidal Cohen | Key Grip |
| Richard L. Cottrell | Key Grip |
| Russell Curtis | Electrician |
| Rick F. Daus Jr. | Electrician |
| Brendan Duffy | Grip |
| Erik Emerson | First Assistant Camera |
| Sean Fisher | Grip |
| Michael D. Fitzgerald | Electrician |
| Josh Harrison | First Assistant "B" Camera |
| Marvin Haven | Grip |
| Ted Hayash | Chief Lighting Technician |
| Stephen Herter | Grip |
| Ken Hoover | Electrician |
| Kevin Jackson | Best Boy Grip |
| Ben Kanegson | Dolly Grip |
| J. Steven Latham | First Assistant "A" Camera |
| Guy Livneh | Second Unit Director of Photography |
| Benjamin Lowell | Second Assistant Camera |
| Tim McGuire | Electrician |
| Patrick K. Meng | Best Boy Grip |
| Chip Monk | Second Assistant Camera |
| Jack Nealy | Best Boy Electric |
| Louis Normandin | Electrician |
| John Oliveri | Second Assistant Camera |
| Richard Ramee | Best Boy Grip |
| Tom Richmond | Additional Photography |
| Kenny Rivenbark | Grip |
| John Sawyer | Electrician |
| Bob Scott | Additional Camera |
| Charlie Seale | Camera Car |
| Samuel Andy Seely | Grip |
| Phil Stevenson | Grip |
| Jonathan Stoll | Electrician |
| Scott Stuart | Best Boy Electric |
| Paige Thomas | First Assistant Camera |
| James D. Travis | Grip |
| Vali Valus | First Assistant Camera |
| Robert Veliky | Second Assistant Camera |
| Michael J. Walsh | Rigging Gaffer |
| Sean R. Wilson | Grip |
| S. Mattieu Workman | Grip |
| John Woruck | Electrician |
| Marc Wostak | Electrician |
| Arthur Yambor | Camera Loader |
| Grayson Austin | "B" Camera Operator |
| Scot A. Nederman-Paul | Camera Loader |
| Kate Nocks | Electrician |
| Sean Tanner | Gaffer |
| Kirk Tedeski | Playback Coordinator |
| Caitlin McKenna | ADR Voice Casting |
| Kimberly Mullen | Casting |
| Mark Mullen | Casting |
| Al Almeida | Wardrobe Assistant |
| Terri Binion | Costumer |
| Amelia Buhrman | Wardrobe Assistant |
| Claudia Combee | Key Set Costumer |
| Jen Haines | Wardrobe Assistant |
| Emily Harris | Costumer |
| Andrea Hathaway | Wardrobe Assistant |
| Unchin Lee | Wardrobe Assistant |
| Sara McInturff | Wardrobe Assistant |
| Judi Stewart | Costumer |
| George Cunningham | Assistant Editor |
| Joe Fineman | Post Production Consulting |
| Bob Fredrickson | Color Timer |
| Darrel Drinkard | Other |
| Michael Nouryeh | Assistant Editor |
| Rick Callan | Location Manager |
| Linda Kai | Location Manager |
| Lucy Olney | Location Scout |
| Mary Ross | Location Assistant |
| Jenée DeAngelis | Music Coordinator |
| Joshua Winget | Music Editor |
| Alice Wymer | Script Supervisor |
| Danny Mortenson | Transportation Coordinator |
| Gabriel London | Epk Director |
