 | Milla Jovovich als Alice |
 | Wentworth Miller als Chris Redfield |
 | Ali Larter als Claire Redfield |
 | Kim Coates als Bennett |
 | Kacey Clarke als Crystal |
 | Shawn Roberts als Albert Wesker |
 | Sergio Peris-Mencheta als Angel |
 | Boris Kodjoe als Luther West |
 | Spencer Locke als K-Mart |
 | Sienna Guillory als Jill Valentine |
 | Norman Yeung als Kim Yong |
 | Ray Olubowale als Axeman |
 | Christopher Kano als Sniper |
| Tatsuya Goke als Sniper |
 | Fulvio Cecere als Wendell |
 | Nobuya Shimamoto als Technician #1 |
 | Denis Akiyama als Captain Hotaka |
| Kenta William Tomeoki als Technician #2 |
| Peter Kosaka als Duty Officer |
| Shin Kawai als Umbrella Sergeant |
 | Mika Nakashima als J Pop Girl |
 | Takato Yamashita als Business Man |
 | Hiromi Okuyama als Kogel Girl Undead |
 | Iain Glen als Dr. Isaacs (archive footage) |
 | Bill Blair als Featured Zombie (uncredited) |
 | Emmanuel Buriez als Zombie (uncredited) |
 | Martin Crewes als Chad Kaplan (uncredited) |
| Jamie Ferenczi als Survivor (uncredited) |
 | Stefen Hayes als Yuri Loginova (uncredited) |
| Brian Jansen als Zombie (uncredited) |
 | Azmyth Kaminski als Zombie (uncredited) |
| Devon Richards als Zombie (uncredited) |
 | Jay Yoo als Japanese soldier (uncredited) |
| Paul W. S. Anderson | Director |
| Paul W. S. Anderson | Producer |
| Jeremy Bolt | Producer |
| Don Carmody | Producer |
| Bernd Eichinger | Producer |
| Samuel Hadida | Producer |
| Robert Kulzer | Producer |
| Niven Howie | Editor |
| Glen MacPherson | Director of Photography |
| Hiroyuki Kobayashi | Producer |
| Victor Hadida | Executive Producer |
| Martin Moszkowicz | Executive Producer |
| Robin D. Cook | Casting |
| Arvinder Greywal | Production Design |
| Suzanne Smith | Casting |
| Denise Cronenberg | Costume Design |
| Azalia Snail | Costume Design |
| Leslie A. Sebert | Makeup Department Head |
| Jo-Ann MacNeil | Makeup Effects |
| Vincent Sullivan | Hair Department Head |
| Carol Hartwick | Key Hair Stylist |
| Brandt Gordon | Art Direction |
| Carolyn A. Loucks | Set Decoration |
| Christina Smith | Makeup Artist |
| Nadia Guglieri | Script Supervisor |
| Dug Rotstein | Script Supervisor |
| Charles Taylor | Armorer |
| Al Vrkljan | Armorer |
| Arthur Rowsell | Assistant Costume Designer |
| Renee Fontana | Assistant Costume Designer |
| Collingwood Brown | Art Department Coordinator |
| Ronald Gilbert | Production Manager |
| Gregor Hutchison | Post Production Supervisor |
| William Cheng | Set Designer |
| Tucker Doherty | Set Designer |
| Vladislav Fedorov | Set Designer |
| Sorin Popescu | Set Designer |
| James Blacker | Location Scout |
| Jonathan Matthews | Location Manager |
| Tracey Savein | Location Scout |
| Kevin Banks | Music Editor |
| Melissa Girotti | Production Coordinator |
| Brian Kobo | Production Coordinator |
| Dave Tommasini | Pilot |
| Jim Holmes | Best Boy Grip |
| Michael Carella | Camera Operator |
| Keith Murphy | Camera Operator |
| Roger Finlay | Camera Operator |
| Perry Hoffman | Camera Operator |
| Carly Brenner | Camera Trainee |
| Julia Seidman | Camera Trainee |
| Jeff Hicks | Camera Trainee |
| Dan Grease White | Camera Trainee |
| Vern Nobles | Aerial Director of Photography |
| David Franco | Second Unit Director of Photography |
| Igor Meglic | Second Unit Director of Photography |
| Ron Renzetti | Dolly Grip |
| John Colavecchia | First Assistant Camera |
| Mark Cyre | First Assistant Camera |
| Colin Akoon | First Assistant Camera |
| Marcel Janisse | First Assistant Camera |
| Brian White | First Assistant Camera |
| Michael Galbraith | Gaffer |
| Gary Deneault | Gaffer |
| Robert Johnson | Key Grip |
| Michael E. Matteson | Key Grip |
| Tom Starnes | Rigging Gaffer |
| Jim Krauter | Rigging Grip |
| Benjamin Smith | Second Assistant Camera |
| Michael Carr | Second Assistant Camera |
| Brian Cassar | Second Assistant Camera |
| Amanda Wojtaszek | Second Assistant Camera |
| Patrick King | Dolly Grip |
| Rafy | Still Photographer |
| Brent Robinson | Underwater Camera |
| Sondra Treilhard | Assistant Hairstylist |
| Burton J. LeBlanc | Assistant Makeup Artist |
| Cliona Furey | Hairstylist |
| Jo-Ann MacNeil | Key Makeup Artist |
| Sandra Wheatle | Makeup Artist |
| Graham Chivers | Prosthetic Makeup Artist |
| Jason Detheridge | Prosthetic Makeup Artist |
| Chris Devitt | Prosthetic Makeup Artist |
| Kyle Glencross | Prosthetic Makeup Artist |
| Iantha Goldberg | Prosthetic Makeup Artist |
| Shane Zander | Prosthetic Makeup Artist |
| Patrick Baxter | Makeup Effects |
| Kelly Kavanagh | Makeup Effects |
| Neil Morrill | Makeup Effects |
| Kelly O'Leary | Makeup Effects |
| Rocky Faulkner | Makeup Effects |
| Richard Redlefsen | Makeup Effects |
| Travis MacKay | ADR Mixer |
| Wade Barnett | ADR Recordist |
| Adam Horley | ADR Recordist |
| Andy Schlegel | ADR Recordist |
| Alan Zielonko | Boom Operator |
| Jill Purdy | Dialogue Editor |
| Steve F.B. Smith | Dolby Consultant |
| Rose Gregoris | First Assistant Sound Editor |
| Steve Baine | Foley Artist |
| Peter Persaud | Foley Recordist |
| Sean Paul Armstrong | Boom Operator |
| John J. Thomson | Sound |
| Coll Anderson | Sound Designer |
| Alex Bullick | Sound Effects Editor |
| Thomas Hayek | Sound Recordist |
| Andrew Stirk | Sound Re-Recording Mixer |
| Andrew Tay | Sound Re-Recording Mixer |
| Mark Zsifkovits | Sound Re-Recording Mixer |
| Stephen Barden | Supervising Sound Editor |
| Hanung Lee | Animation |
| Frankie Stellato | Animation |
| Mehta Apul | Animation |
| Peter Dydo | Animation |
| Gavin Soares | Animation |
| Emrah Gonulkirmaz | Animation |
| Daniel Rhein | CG Artist |
| Gabriel Tremblay-Beauvais | CG Artist |
| Craig Calvert | CG Supervisor |
| Dominic Remane | CG Supervisor |
| Louis Katz | CG Supervisor |
| Robert Greb | Compositing Supervisor |
| Ovidiu Cinazan | Compositor |
| Kaitlyn Lovatt | Compositor |
| Prasenjit Mukherjee | Compositor |
| Chris Ryan | Compositor |
| Kamal Kumar Tiwari | Compositor |
| Mrityunjay Singh | Compositor |
| Mahapatro Kalpana | Concept Artist |
| Mark Fordham | Concept Artist |
| Sébastien Moreau | Digital Effects Supervisor |
| Engin Arslan | Lighting Artist |
| Allan Henderson | Lighting Artist |
| Brian Dignadice | Lighting Artist |
| Mathew Borrett | Matte Painter |
| Ken McCuen | Matte Painter |
| Ravindra Tamhankar | Matte Painter |
| Peter Toufidis | Matte Painter |
| Milan Schere | Matte Painter |
| Tracey McLean | Matte Painter |
| Stéphan Brisson | Matte Painter |
| Marilyne Fleury | Matte Painter |
| Éric Hamel | Matte Painter |
| Ludovic Iochem | Matte Painter |
| Moika Sabourin | Matte Painter |
| Frederic St-Arnaud | Matte Painter |
| Robin Tremblay | Matte Painter |
| Joell Xin Zhow | Matte Painter |
| Kevin Labanowich | Senior Animator |
| Mathieu Raynault | Visual Effects Art Director |
| Becca Donohoe | Visual Effects Coordinator |
| Leann Harvey | Visual Effects Coordinator |
| Benjamin Mossman | Visual Effects Coordinator |
| Julien Tremblay | Visual Effects Coordinator |
| Sarah Barber | Visual Effects Coordinator |
| Josiane O'Rourke | Visual Effects Coordinator |
| Mike Barber | Visual Effects Editor |
| Florian Vera | Visual Effects Editor |
| Eric J. Robertson | Visual Effects Producer |
| Tavia Charlton | Visual Effects Producer |
| Justin Smith | Visual Effects Production Assistant |
| Geneviève Morin | Visual Effects Production Assistant |
| Gabriel Roy | Visual Effects Production Assistant |
| Lee Ann Simard | Visual Effects Production Assistant |
| Mike Borrett | Visual Effects Production Manager |
| Eric Robinson | Visual Effects Production Manager |
| Dennis Berardi | Visual Effects Supervisor |
| Ian Hunter | Visual Effects Supervisor |
| Wojciech Zielinski | Visual Effects Supervisor |
| Tom Turnbull | Visual Effects Supervisor |
| Lara Johnston | Assistant Editor |
| Harry Muller | Color Timer |
| Chris Wallace | Digital Intermediate Colorist |
| Motassem Younes | Digital Intermediate Editor |
| Peter Armstrong | Digital Intermediate Producer |
| Lee Hughes | Digital Intermediate |
| Ben Howdeshell | First Assistant Editor |
| Dawn M. Stoliar | First Assistant Editor |
| Tom Hajdu | Original Music Composer |
| Andy Milburn | Original Music Composer |
| Angelica Lisk-Hann | Stunts |
| Stephannie Hawkins | Stunts |
| Mary Ann Stevens | Stunts |
| Karen Leach | Stunts |
| Joanne Leach | Stunts |
| Vanessa King | Stunts |
| Liise Keeling | Stunts |
| Taryn Ash | Stunts |
| Rick Forsayeth | Stunt Coordinator |
| Paul W. S. Anderson | Writer |
| tomandandy | Music |
| Dana Howes | Transportation Coordinator |
