 | Milla Jovovich als Alice |
 | Sienna Guillory als Jill Valentine |
 | Oded Fehr als Carlos Olivera |
 | Thomas Kretschmann als Major Cain |
 | Sophie Vavasseur als Angela "Angie" Ashford |
 | Razaaq Adoti als Sgt. Peyton Wells |
 | Jared Harris als Dr. Ashford |
 | Mike Epps als L.J. |
 | Sandrine Holt als Terri Morales |
 | Matthew G. Taylor als Nemesis |
 | Zack Ward als Sergente Nicholai Ginovaeff |
 | Iain Glen als Dr. Isaacs |
 | Dave Nichols als Captain Henderson |
 | Stefen Hayes als Yuri Loginova |
 | Geoffrey Pounsett als Mackenzie |
 | Shaun Austin-Olsen als Priest |
| Geny Walters als Mrs. Priest |
 | Megan Fahlenbock als Marla Mapes |
| Robert Morelli als Director |
 | Aaron Abrams als Assistant |
| Chris Benson als Lance Halloran |
| Martin Martinuzzi als Overworked Detective |
| Brad Borbridge als Overworked Sergeant |
 | Frank Chiesurin als Sniper |
| Ron Benjamin als Bum |
 | Kevin Hare als Home Owner / Scientist |
| Heather Dick als Older Woman |
| Alex Courey als Suit #1 |
| John Detoro als Suit #2 |
| Billy Khoury als Suit #3 |
 | Sava Drayton als Suit #4 |
| Nicole Ann St. Hill als Undead Gaudy Hooker |
 | Ricardo Betancourt als Burly Cop #1 |
| Ted Ludzik als Burly Cop #2 |
 | Michelle Latimer als Tech #1 |
| Catherine Burdon als Tech #2 |
| Rebecca Sims als Undead Little Girl |
| Christopher Weedon als Undead Little Boy #1 |
 | Billy Parrott als Security Guard |
| Derek Aasland als Featured Graveyard Undead 1 (as Derek Asland) / Featured "Surplus" Undead #3 |
| Richard Harte als Featured Graveyard Undead 2 / Featured Rooftop Undead #2 |
| Simon Girard als Chopper Pilot #1 |
| Dean Copkov als Chopper Pilot #2 |
 | Jeff White als Chopper Pilot #4 |
 | Timothy Burd als Refugee #1 |
| Natalie Charles als Refugee #2 |
| Pierre Henry als Screaming Soldier |
 | Jim Codrington als Lieutenant |
| Deborah Valente als Tech |
| Malcolm Fiddick als Reflection Undead |
| Malcolm Xerxes als Featured City Hall Undead #1 |
 | Michy Gustavia als Featured City Hall Undead #2 |
| Shane Mackinnon als Featured City Hall Undead #3 |
| Ed Fielding als Featured City Hall Undead #4 |
 | Stephen R. Hart als Featured City Hall Undead #5 |
| Sandi Stahlbrand als Reporter #1 |
 | Christopher Marren als Guard |
 | Tom Gerhardt als Zombie Dad |
 | Amber Marshall als Daughter |
| Venice Grant als Undead Stripper #1 |
| Ana Danilina als Undead Stripper #2 |
| Ingrid Johansson als Featured IV Woman Undead |
| Jeffrey Weedon als Undead Little Boy #2 |
 | Melanie Tonello als Featured Undead Kid #1 |
| Annabel Chu als Featured Undead Kid #2 |
| Joe Jefferson als Featured Undead Kid #3 |
| Heather Braaten als Featured Rooftop Undead #1 |
 | Joe Silvaggio als Featured Rooftop Undead #3 (as Joey Beck) |
| Eric Morgan als Featured Undead City Walker #1 |
 | Gene Pyrz als Featured Undead City Walker #2 |
 | Torquil Colbo als Featured Undead City Walker #3 |
| Joan Moore als Featured Undead City Walker #4 |
| Peter C. Wylde als Featured Undead City Walker #5 |
| Garry Geddes als Featured Undead City Walker #6 |
| Dionne Renee als Featured Undead City Walker #7 |
| Janessa Crimi als Featured Undead Kid #4 |
| Dan Ellery als Featured Graveyard Undead 1 |
| Sharon Moore als Featured "Surplus" Undead #2 |
| Jo Chim als Network Anchor #1 |
| Bobby Prochaska als Network Anchor #2 |
| Jazz Mann als Reporter #2 |
 | Larissa Gomes als Reporter #3 |
| Gregory White als Lab Technician |
 | Roman Podhora als Leader Umbrella Guard |
| Brian King als Umbrella Gate Guard |
| Rob Hall als Featured School Undead |
 | John Migliore als Zombie (uncredited) |
| Yaron Betan als Featured Racoon Police (uncredited) |
 | Martin Crewes als Chad Kaplan (uncredited) |
| Brandon Marc Gagne als Zombie (uncredited) |
 | Ryan McCluskey als M. Grey (uncredited) |
| Michael C. Newsome als Detective (uncredited) |
| Avi Salem als Refugee (uncredited) |
 | Alexander Witt als Umbrella Sniper (uncredited) |
| Alexander Witt | Director |
| Paul W. S. Anderson | Producer |
| Jeremy Bolt | Producer |
| Don Carmody | Producer |
| Dennis Chapman | Line Producer |
| Bernd Eichinger | Executive Producer |
| Samuel Hadida | Executive Producer |
| Victor Hadida | Executive Producer |
| Robert Kulzer | Executive Producer |
| Tina Gerussi | Casting |
| Mary E. McLeod | Costume Design |
| Eddie Hamilton | Editor |
| Paul D. Austerberry | Production Design |
| Nigel Churcher | Art Direction |
| Robyn Ray | Casting Consultant |
| Daniel S. Kletzky | Associate Producer |
| Hiroyuki Kobayashi | Associate Producer |
| Martin Moszkowicz | Executive Producer |
| Jeff Danna | Original Music Composer |
| Derek Rogers | Director of Photography |
| Christian Sebaldt | Director of Photography |
| Fabian Jonathan | Art Direction |
| Steven Essam | Set Decoration |
| David G. Fremlin | Assistant Art Director |
| Kirk Cheney | Construction Coordinator |
| Philip Spurvey | Location Scout |
| Stephen Paisley | Painter |
| Martin L. Mercer | Sculptor |
| Mayumi Konishi-Valentine | Set Designer |
| Roberto W. Contreras D. | Camera Operator |
| Tony Magaletta | First Assistant Camera |
| Sean Jensen | Steadicam Operator |
| Rolf Konow | Still Photographer |
| Teresa Buccione | Key Hair Stylist |
| Elia Cmiral | Additional Music |
| Charles Taylor | Armorer |
| Shawney Cohen | CG Supervisor |
| Sharon Moore | Choreographer |
| Simone Lindo | Craft Service |
| Julie Adams | Dialect Coach |
| Kyle Menzies | Digital Effects Supervisor |
| John Coles | Driver |
| Dave Axford | Makeup Effects |
| Gregor Hutchison | Post Production Supervisor |
| Matthew Lammerich | Scenic Artist |
| John Jay Hebert | Second Unit Cinematographer |
| Rick Leary | Sequence Supervisor |
| Steve Holt | Set Medic |
| Steve Lucescu | Stunt Coordinator |
| Nick Alachiotis | Stunts |
| Vladimir Popovic | Systems Administrators & Support |
| Joel Green | Unit Publicist |
| Joel Harris | Utility Stunts |
| Anthony Nocera | Video Assist Operator |
| Sue Len Quon | Visual Effects Editor |
| Alan Goluboff | Assistant Director |
| Carol Pears | Script Supervisor |
| Claire McGrane | Digital Intermediate |
| John O'Boyle | Best Boy Electric |
| Kevin Barnes | Electrician |
| Kevin Alanthwaite | Gaffer |
| Alex Murtaza | Lighting Artist |
| Ian Foulds | Rigging Gaffer |
| Tracy Shaw | Rigging Grip |
| Jonathan Matthews | Location Manager |
| Kimberley Bradley | Production Coordinator |
| Nan Skiba | Production Manager |
| Chris Ellefson | Researcher |
| Denis Bellingham | Boom Operator |
| Alex Bullick | First Assistant Sound Editor |
| Tony Lewis | Music Editor |
| Liz Gallacher | Music Supervisor |
| Nicholas Dodd | Orchestrator |
| Craig Henighan | Sound Designer |
| Kevin Banks | Sound Editor |
| Nathan Robitaille | Sound Effects Editor |
| Todd Beckett | Sound Re-Recording Mixer |
| Craig Henighan | Supervising Sound Editor |
| Barb Benoit | Digital Compositors |
| Steve Parsons | Visual Effects |
| Sarah McMurdo | Visual Effects Coordinator |
| Mark Halsall | Visual Effects Producer |
| Dennis Berardi | Visual Effects Supervisor |
| Rob McCallum | Storyboard |
| John Charles | Software Engineer |
| Clyde Miller | Aerial Camera |
| Monty Montgomerie | Key Grip |
| Marie Nardella | Key Makeup Artist |
| Brian Anderson | 3D Animator |
| Nick Fischer | Set Dresser |
| Glyn Tebbutt | Title Designer |
| Renée Pilgrim | Camera Loader |
| Thomas Care | Dolly Grip |
| Randy Burbidge | Grip |
| Madeline Brian | Costume Illustrator |
| Adam Smith | Key Costumer |
| Paul Jones | Makeup Effects Designer |
| Shelley Cook | Aerial Coordinator |
| Guillaume Carpentier | Pilot |
| Tom Blacklock | Pyrotechnician |
| Pierre Henry | First Assistant Director |
| Grant Lucibello | Second Assistant Director |
| Cameron Kuc | Third Assistant Director |
| Tom Mayclim | Negative Cutter |
| Darron Leiren-Young | Assistant Production Coordinator |
| Ian Maxwell | Assistant Production Manager |
| Roberta Romano | Casting Assistant |
| Stephen Barden | ADR Editor |
| Russ Miller | Musician |
| Jeff Danna | Music Score Producer |
| Jean-Louis Billard | 3D Artist |
| Emily Cobb | 3D Modeller |
| Conrad Dueck | 3D Supervisor |
| Mark Palowich | CG Animator |
| Monica Sandstede | Story Editor |
| Lorraine Greenwood | Assistant Makeup Artist |
| Elayne Alexander | Costume Set Supervisor |
| Liza Vespi | Continuity |
| Spiro Razatos | Second Unit Director |
| Barton Rendulic | Concept Artist |
| Ian Fraser | Head Carpenter |
| Matt Middleton | Second Assistant Art Director |
| Monica Wier | Set Supervisor |
| Temple Clark | Storyboard Artist |
| Adam Sharpe | ADR Mixer |
| Matthew McKenzie | ADR Recordist |
| Gina Gyles | Assistant Foley Artist |
| Steve Baine | Foley Artist |
| Peter Persaud | Foley Recordist |
| Rose Gregoris | Second Assistant Sound |
| Rocco Larizza | Special Effects Assistant |
| Joel Crimi | Special Effects Technician |
| Barb Benoit | Visual Effects Compositor |
| Jennifer Wood | Visual Effects Editor |
| Patrick White | Visual Effects Production Assistant |
| Carl Beyer | Visual Effects Production Manager |
| Murat Akser | Assistant Camera |
| Craig Cooper | Clapper Loader |
| Deanna Cadette | Second Assistant Camera |
| Adam Glasman | Colorist |
| Lorraine Samuel | Post Production Coordinator |
| Drazen Baric | Assistant Location Manager |
| Fernando DaSilva | Location Production Assistant |
| Nicholas Dodd | Conductor |
| Ann Fitzgerald | Accountant |
| Anthony Valenti | Assistant Accountant |
| Baron Evans | Assistant Production Coordinator |
| Karl Sisson | CG Artist |
| Derek Aasland | Choreographer |
| Noel Hooper | Compositing Supervisor |
| Miriam Laurence | Dialogue Coach |
| Jody Johnson | Digital Compositor |
| Jane Rogers | Extras Casting |
| Dylaine Bovaird | Extras Casting Assistant |
| Hope White | First Assistant Accountant |
| Paul Marshall | Head Driver |
| James F. Breithaupt | Key Accountant |
| Pete Daprato | Key Rigging Grip |
| Skyler Wilson | Key Special Effects |
| Kris Wood | Playback Coordinator |
| David McElroy | Production Assistant |
| Jan Hogevold | Production Executive |
| Jillian Sabean | Production Secretary |
| Tami Thomson | Travel Coordinator |
| Alicia Turner | Stunts |
| Melissa R. Stubbs | Stunts |
| Mary Ann Stevens | Stunts |
| Vanessa Reid | Stunts |
| Cheryl Quiacos | Stunts |
| Patrizia Quas | Stunts |
| Sue Parker | Stunts |
| Isolde O'Neill | Stunts |
| Janet Morin | Stunts |
| Stéphane Lefebvre | Stunts |
| Joanne Leach | Stunts |
| Kym Kristalie | Stunts |
| Catherine Gourdier | Stunts |
| Monique Ganderton | Stunts |
| Megan Fahlenbock | Stunts |
| Maxine Dumont | Stunts |
| Taryn Ash | Stunts |
| Paul W. S. Anderson | Writer |
| Nick Bacon | Best Boy Grip |
| Jean Frenette | Stunts |
