 | Frank Welker als Scooby-Doo / Fred Jones (voice) |
 | Grey DeLisle als Daphne Blake / Black Canary (voice) |
 | Matthew Lillard als Shaggy Rogers (voice) |
 | Kate Micucci als Velma Dinkley (voice) |
 | Diedrich Bader als Batman (voice) |
 | Jeff Bennett als Joker (voice) |
 | Jeffrey Combs als The Question (voice) |
 | John DiMaggio als Aquaman / Crimson Cloak (voice) |
 | Nika Futterman als Catwoman (voice) |
 | Nicholas Guest als Martian Manhunter (voice) |
 | John Michael Higgins als The Riddler (voice) |
 | Tom Kenny als Penguin / Plastic Man (voice) |
 | Kevin Michael Richardson als Clayface / Detective Chimp (voice) |
 | Tara Strong als Harley Quinn / Poison Ivy (voice) |
 | Fred Tatasciore als Harvey Bullock (voice) |
| Jake Castorena | Director |
| Amy McKenna | Line Producer |
| Paul Giacoppo | Teleplay |
| Bob Kane | Characters |
| Bill Finger | Characters |
| James Tucker | Supervising Producer |
| William Hanna | Characters |
| Sam Register | Executive Producer |
| Benjamin Melniker | Executive Producer |
| Joseph Barbera | Characters |
| Molly Yahr | Editor |
| Michael Jelenic | Producer |
| Michael Uslan | Executive Producer |
| James Tucker | Story |
| Christopher D. Lozinski | Editor |
| Sam Riegel | Casting |
| Kristopher Carter | Original Music Composer |
| Lolita Ritmanis | Original Music Composer |
| Michael McCuistion | Original Music Composer |
| Paul Norris | Characters |
| Joe Samachson | Characters |
| Joe Certa | Characters |
| Paul Dini | Characters |
| Bruce Timm | Characters |
| Steve Ditko | Characters |
| Matthew Mahoney | Associate Producer |
| Melissa Lohman | Assistant Production Manager |
| Dan Haskett | Character Designer |
| Lynell Forestall | Character Designer |
| Brian Uchida | Prop Designer |
| Jay Hong | Background Designer |
| Pakin Liptawat | Background Designer |
| Alan Caldwell | Storyboard Artist |
| Brendan Clogher | Storyboard Artist |
| Shannon Eric Denton | Storyboard Artist |
| Lynell Forestall | Storyboard Artist |
| Stephen Jones | Storyboard Artist |
| David Maximo | Storyboard Artist |
| Zak Plucinski | Storyboard Artist |
| Ruben Procopio | Storyboard Artist |
| Matthew Girardi | Supervising Technical Director |
| Anthony Akira Andrade | Animation |
| Aaron Chavda | Animation |
| Brett Hardin | Animation |
| Jason Plapp | Animation |
| Greg Emerson | Other |
| Brittany Canasi | Post-Production Manager |
| Rebecca Unger | Post-Production Manager |
| Jeff O. Collins | Other |
| Wilson Martinez | Other |
| Fred Salinas | Other |
| Mark A. Keatts | Supervising Dialogue Editor |
| Mark A. Keatts | ADR Editor |
| David M. Cowan | Dialogue Editor |
| David M. Cowan | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Patrick J. Foley | Dialogue Editor |
| Patrick J. Foley | ADR Editor |
| Michael Garcia | Dialogue Editor |
| Michael Garcia | ADR Editor |
| David Cowles | Online Editor |
| Melissa Bailey | Assistant Editor |
| Daphne Fei | Administration |
| Liz Carroll | Administration |
| Yadira Davis | Production Accountant |
| Debbie Lindquist | Production Accountant |
| Maral Simonian | Production Accountant |
| Athena Wingate | Production Accountant |
| Bobbie Page | Production Supervisor |
| Ed Adams | Production Manager |
| Jay Bastian | Executive In Charge Of Production |
| Joe Ruby | Thanks |
| Ken Spears | Thanks |
