 | Frank Welker als Scooby-Doo / Fred Jones / Msicellaneous Bikers (voice) |
 | Mindy Cohn als Velma Dinkley (voice) |
 | Grey DeLisle als Daphne Blake (voice) |
 | Matthew Lillard als Shaggy Rogers / Shaky Joe (voice) |
 | Cathy Cavadini als Faith (voice) |
 | John DiMaggio als Friz / GPS / Msicellaneous Bikers (voice) |
 | Michael Gough als Mr. Babbit / Blair / Grad Student #1 / Bikers (voice) |
 | Matthew Gray Gubler als Winsor (voice) |
 | Finola Hughes als Professor Svankmajer (voice) |
 | Maulik Pancholy als Doctor (voice) |
 | Kevin Michael Richardson als Tex / Cop #2 / Grad Student #3 / Bikers (voice) |
 | Fred Willard als Mr. Hubley (voice) |
 | Dave Wittenberg als Policeman / Cop #1 / Grad Student #2 / Bikers (voice) |
 | Gwendoline Yeo als Ms. Deitch (voice) |
| Ethan Spaulding | Director |
| Doug Langdale | Writer |
| Spike Brandt | Producer |
| Tony Cervone | Producer |
| Robert J. Kral | Original Music Composer |
| Andrea Romano | Casting |
| Damon P. Yoches | Editor |
| Ted Blackman | Art Direction |
| Eric Semones | Art Direction |
| Alan Burnett | Co-Producer |
| Wade Wisinski | Line Producer |
| Sam Register | Executive Producer |
| Dan Haskett | Character Designer |
| Jerome K. Moore | Character Designer |
| Judge Plummer | Production Manager |
| Scott Shinick | Post-Production Manager |
| Jeff O. Collins | Other |
| Sarah Baluch | Other |
| Mark A. Keatts | Supervising Dialogue Editor |
| Mark A. Keatts | ADR Editor |
| Kelly Ann Foley | Dialogue Editor |
| Kelly Ann Foley | ADR Editor |
| Patrick J. Foley | Dialogue Editor |
| Patrick J. Foley | ADR Editor |
| Michael Garcia | Dialogue Editor |
| Michael Garcia | ADR Editor |
| Otis Van Osten | Sound Supervisor |
| Jeff Shiffman | Sound Effects |
| Jeff Shiffman | Sound Editor |
| Christopher D. Lozinski | Online Editor |
| Carlos Sanches | Sound Re-Recording Mixer |
| Christine H. Luethje | Music Editor |
| Niki Sherrod | Other |
| Nicole Martin | Administration |
| Liz Carroll | Administration |
| Bobbie Page | Production Supervisor |
| Ed Adams | Production Manager |
| Joe Ruby | Thanks |
| Ken Spears | Thanks |
