 | Frank Welker als Scooby-Doo / Fred Jones / Self |
 | Matthew Lillard als Norville 'Shaggy' Rogers / Self - Voice of "Shaggy" |
 | Grey DeLisle als Daphne Blake / Self - Voice of "Daphne" |
 | Kate Micucci als Velma Dinkley / Self - Voice of "Velma" |
 | Janel Parrish als Janel Parrish - Host |
 | Jerry Beck als Self - Animation Historian and Author |
 | David Silverman als Self - Director and Producer, "The Simpsons" |
 | Seth Green als Self - Actor, "Scooby-Doo 2: Monsters Unleashed" |
 | Tony Cervone als Self - Director, "Scoob!" / Showrunner, "Mystery Incorporated" |
 | Tom Sito als Self - Animator, Historian |
 | Joseph Barbera als Self - Hanna-Barbera Co-Founder (archive footage) |
 | Mary Lynn Rajskub als Producer Bryn |
 | Baron Vaughn als Bram Penobscot - Scooby's Vocal Coach |
 | Casey Kasem als Shaggy Rogers (voice) (archive footage) |
 | Diedrich Bader als Batman - Concerned Citizen (voice) |
 | 'Weird Al' Yankovic als Self - Singer, Songwriter, Accordionist |
 | Olivia Liang als Olivia Liang - Shaggy's Martial Arts Trainer |
 | Karamo Brown als Costume Designer Carter |
 | Mitch Watson als Self - Showrunner, "Mystery Incorporated" |
 | Cheri Oteri als Lazlo the Mechanic |
 | Brendan Jennings als Arlo Davidsonman - Team Scooby |
| Vadym Krasnenko als Snow Ghost |
| Lindsey Alena als Additional Voices (voice) |
| Dominic Catrambone als Additional Voices (voice) |
 | Fred Tatasciore als Additional Voices (voice) |
| Jonathan Stern | Executive Producer |
| Jonathan Stern | Writer |
| Jonathan Stern | Director |
| Jerry Beck | Thanks |
| Tony Cervone | Thanks |
| Seth Green | Thanks |
| David Silverman | Thanks |
| Tom Sito | Thanks |
| Mitch Watson | Thanks |
| 'Weird Al' Yankovic | Thanks |
| David Soldinger | Producer |
| Franny Baldwin | Producer |
| Jamie Conklin | Associate Producer |
| Franny Baldwin | Other |
| Cassandra B. Laymon | Other |
| Marc Newland | Other |
| Dan Adlerstein | Director of Photography |
| Jamie Conklin | Editor |
| Trish Hadley | Post Producer |
| Matt Novack | Original Music Composer |
| Greg Martin | Original Music Composer |
| Bryn Carter | Costume Design |
| Mandy Pauline Harris | Costume Supervisor |
| Morgan McDonnell | Makeup Department Head |
| Leah Renee Rutledge | Hair Department Head |
| Caroline Stratton | Property Master |
| Jack Brungardt | Production Coordinator |
| Robbie Stern | Post Production Supervisor |
| Victor Parra | Assistant Editor |
| Payam Yazdandoost | First Assistant Camera |
| Will Elder | Gaffer |
| Eduardo Barraza | Key Grip |
| Elena Antzon | Script Supervisor |
| Zsolt Magyar | Sound Mixer |
| Michael Off | Transportation Captain |
| Claudia Geldis | Production Controller |
| Ryan Kramer | Storyboard Artist |
| Tim Blair | Animation |
| Bobbie Page | Production Executive |
| Daniel Ridgers | Line Producer |
| Aisha Clarke | Production Manager |
| Mac Whiting | Supervising Animation Director |
| Brianne Van Tuyle | Animation |
| Tracy Lee | Character Designer |
| Will Lehr | Online Editor |
| Olney Atwell | Graphic Designer |
| Charlie Tucker | Colorist |
| Ted Nichols | Other |
