 | Basil Rathbone als Sherlock Holmes |
 | Nigel Bruce als Dr. John H. Watson |
 | Alan Mowbray als Major Duncan-Bleek |
 | Dennis Hoey als Inspector Lestrade |
 | Renee Godfrey als Vivian Vedder |
 | Frederick Worlock als Prof. William Kilbane |
 | Mary Forbes als Lady Margaret Carstairs |
 | Skelton Knaggs als Sands - Moran's Accomplice |
 | Billy Bevan als Conductor Taking Tickets |
 | Geoffrey Steele als Roland Carstairs |
| Janet Murdoch als Mrs. Shallcross (uncredited) |
 | Gerald Hamer als Alf Shallcross (uncredited) |
 | Leyland Hodgson als Senior Conductor (uncredited) |
 | Harry Cording als Mock the Undertaker (uncredited) |
| Charles Knight als Baggage Car Guard (uncredited) |
| Boyd Davis als "Inspector MacDonald" (uncredited) |
| Bobby Wissler als Mock Jr. (uncredited) |
| Gilbert Allen als Dining Car Steward (uncredited) |
 | C. Aubrey Smith als Elderly gentleman on train station (uncredited) |
| Roy William Neill | Director |
| Frank Gruber | Screenplay |
| Saul A. Goodkind | Editor |
| Arthur Conan Doyle | Story |
| Maury Gertsman | Director of Photography |
| Milton Rosen | Music Director |
| John B. Goodman | Art Direction |
| Abraham Grossman | Art Direction |
| Bernard B. Brown | Sound Director |
| Russell A. Gausman | Set Decoration |
| Carl Lawrence | Set Decoration |
| Vera West | Costume Design |
| Carmen Dirigo | Hairstylist |
| Jack Pierce | Makeup Department Head |
| Howard Benedict | Executive Producer |
| Roy William Neill | Producer |
