 | James Baskett als Uncle Remus / Br'er Fox (Voice) |
 | Ruth Warrick als Sally |
 | Bobby Driscoll als Johnny |
 | Luana Patten als Ginny |
 | Lucile Watson als Grandmother |
 | Hattie McDaniel als Aunt Tempy |
 | Erik Rolf als John (as Eric Rolf) |
 | Glenn Leedy als Toby |
 | Mary Field als Mrs. Favers |
 | Anita Brown als Maid |
| Georgie Nokes als Jake Favers |
| Gene Holland als Joe Flavers |
 | Nick Stewart als Br'er Bear (voice) |
| Johnny Lee als Br'er Rabbit (voice) |
| Helen Crozier als Mother Possum (voice) |
| Babette De Castro als Bird Voices (voice) (uncredited) |
| Cherie De Castro als Bird Voices (voice) (uncredited) |
| Peggy De Castro als Bird Voices (voice) (uncredited) |
 | Roy Glenn als Br'er Frog (voice) (uncredited) |
 | Clarence Nash als Mr. Bluebird (voice) (uncredited) |
| Olivier Urbain als Mr. Favers (uncredited) |
 | Ernestine Wade als Butterfly (uncredited) |
| Wilfred Jackson | Director |
| Harve Foster | Director |
| Dalton S. Reymond | Story |
| Joel Chandler Harris | Novel |
| Dalton S. Reymond | Screenplay |
| Maurice Rapf | Screenplay |
| Morton Grant | Screenplay |
| Les Clark | Animation |
| Mary Blair | Conceptual Design |
| Walt Disney | Producer |
| Perce Pearce | Associate Producer |
| Gregg Toland | Director of Photography |
| Bill Peet | Story |
| Ralph Wright | Story |
| Vernon Stallings | Story |
| Daniele Amfitheatrof | Music Arranger |
| Paul J. Smith | Music Arranger |
| Charles Wolcott | Music |
| William Morgan | Editor |
| Perry Ferguson | Art Direction |
| Jack Atwood | Assistant Director |
| William McGarry | Assistant Director |
| Elmer Plummer | Art Designer |
| Irving W. Sindler | Props |
| Fred Lau | Sound Recordist |
| C.O. Slyfield | Sound Director |
| Harold J. Steck | Sound Recordist |
| Gloria Gottschalk | Sound Editor |
| George Lowerre | Sound Engineer |
| Brad Case | Animation |
| Blaine Gibson | Animation |
| Ub Iwerks | Visual Effects |
| Joshua Meador | Animation |
| George Rowley | Animation |
| Frederic Leahy | Production Manager |
| Mary Wills | Costume Design |
| Bob Broughton | Camera Operator |
| Vic Jones | Gaffer |
| F. Bud Mautino | Camera Operator |
| Hal Ambro | Animation |
| Ken Anderson | Supervising Art Director |
| Philip Barber | Art Direction |
| Al Coe | Animation |
| Marc Davis | Animation Director |
| Al Dempster | Background Designer |
| Harold Doughty | Art Direction |
| Hugh Hennesy | Art Direction |
| Ray Huffine | Background Designer |
| Ralph Hulett | Background Designer |
| Ollie Johnston | Animation Director |
| Milt Kahl | Animation Director |
| Hal King | Animation |
| Rudy Larriva | Animation |
| Eric Larson | Animation Director |
| John Lounsbery | Animation Director |
| Don Lusk | Animation |
| Brice Mack | Background Designer |
| Tom Massey | Animation |
| Murray McClellan | Animation |
| Cliff Nordberg | Animation |
| Ken O'Brien | Animation |
| Charles Philippi | Art Direction |
| Ed Starr | Background Designer |
| Harvey Toombs | Animation |
| Wilma Baker | Painter |
| Phyllis Barnhart | Painter |
| Rudy Cataldi | Animation |
| Virginia Fontanella | Painter |
| Myrna Gibbs | Painter |
| Grace Godino | Painter |
| Eadie Hofmann | Painter |
| Jeanne Lee Keil | Painter |
| Ann Lord | Painter |
| Dick N. Lucas | Animation |
| Clarke Mallery | Animation |
| Paul Murry | Animation |
| Buf Nerbovig | Painter |
| Bill Peet | Animation Coordinator |
| Dodie Roberts | Painter |
| Carmen Sanderson | Painter |
| Al Stetter | Animation |
| Mary Tebb | Painter |
| Margaret Trinidade | Painter |
| Val Vreeland | Painter |
| Joyce Walker | Painter |
| Xenia | Animation |
| Natalie Kalmus | Colorist |
| George Cave | Color Timer |
| Ken Darby | Music Director |
| Edward H. Plumb | Orchestrator |
| Charles Wolcott | Music Director |
| Ethmer Roten | Musician |
| Al Teeter | Music Editor |
| Mitchell Kovaleski | Color Assistant |
| Allie Wrubel | Songs |
| Ray Gilbert | Songs |
| Foster Carling | Songs |
| Sam Coslow | Songs |
| Eliot Daniel | Songs |
| Hy Heath | Songs |
| Arthur Johnston | Songs |
| Johnny Lange | Songs |
| Robert MacGimsey | Songs |
| Ken Darby | Songs |
