 | Shameik Moore als Miles Morales / Spider-Man (voice) |
 | Jake Johnson als Peter B. Parker / Spider-Man (voice) |
 | Hailee Steinfeld als Gwen Stacy / Spider-Woman (voice) |
 | Mahershala Ali als Uncle Aaron / Prowler (voice) |
 | Brian Tyree Henry als Jefferson Davis (voice) |
 | Lily Tomlin als Aunt May (voice) |
 | Luna Lauren Velez als Rio Morales (voice) |
 | Zoë Kravitz als Mary Jane (voice) |
 | John Mulaney als Peter Porker / Spider-Ham (voice) |
 | Kimiko Glenn als Peni Parker (voice) |
 | Nicolas Cage als Peter Parker / Spider-Man Noir (voice) |
 | Kathryn Hahn als Olivia 'Liv' Octavius / Doc Ock (voice) |
 | Liev Schreiber als Wilson Fisk / Kingpin (voice) |
 | Chris Pine als Peter Parker / Spider-Man (voice) |
 | Natalie Morales als Miss Calleros (voice) |
 | Edwin H. Bravo als Brooklyn Visions Security Guard (voice) |
 | Oscar Isaac als Interesting Person #1 (voice) |
 | Greta Lee als Interesting Person #2 (voice) |
 | Stan Lee als Stan (voice) |
 | Jorma Taccone als Green Goblin / Last Dude (voice) |
 | Joaquín Cosío als Scorpion (voice) |
 | Marvin 'Krondon' Jones III als Tombstone (voice) |
 | Kim Yarbrough als Scientist in Cafeteria (voice) |
 | Lake Bell als Vanessa Fisk (voice) |
 | Jessica Mikayla Adams als Brooklyn Friend (voice) |
 | Gredel Berrios Calladine als Brooklyn Friend (voice) |
 | Sarah D. Cole als Brooklyn Friend (voice) |
| Kelby Joseph als Brooklyn Friend (voice) |
 | Mimi Davila als Brooklyn Visions Student (voice) |
 | Claudia Choi als Brooklyn Visions Teacher (voice) |
 | Melanie Haynes als Brooklyn Visions Teacher (voice) |
 | Joseph Izzo als Brooklyn Visions Teacher (voice) |
 | Nick Jaine als Brooklyn Visions Teacher (voice) |
 | Muneeb Rehman als Brooklyn Visions Teacher (voice) |
 | Carlos Zaragoza als Brooklyn Visions Teacher (voice) |
 | Post Malone als Brooklyn Bystander (voice) |
| David Applebee als Additional Voices (voice) |
 | Juan Carlos Arvelo als Additional Voices (voice) |
| Adam Brown als Additional Voices (voice) |
 | Jon Bruno als Additional Voices (voice) |
 | Darcy Rose Byrnes als Additional Voices (voice) |
 | Oscar Camacho als Additional Voices (voice) |
 | June Christopher als Additional Voices (voice) |
 | Alycia Cooper als Additional Voices (voice) |
 | Michelle Jubilee Gonzalez als Additional Voices (voice) |
 | Terrence Hardy Jr. als Additional Voices (voice) |
 | Bridget Hoffman als Additional Voices (voice) |
 | Rif Hutton als Additional Voices (voice) |
 | Miguel Jiron als Additional Voices (voice) |
 | Harrison Knight als Additional Voices (voice) |
 | Lex Lang als Additional Voices (voice) |
 | Donna Lynn Leavy als Additional Voices (voice) |
| Andrew Leviton als Additional Voices (voice) |
 | Caitlin McKenna als Additional Voices (voice) |
 | Scott Menville als Additional Voices (voice) |
 | Christopher Miller als Additional Voices (voice) |
 | Arthur Ortiz als Additional Voices (voice) |
 | Juan Pacheco als Additional Voices (voice) |
 | Devika Parikh als Additional Voices (voice) |
 | Shakira Ja'nai Paye als Additional Voices (voice) |
 | Courtney Peldon als Additional Voices (voice) |
 | Chrystee Pharris als Additional Voices (voice) |
 | Jacqueline Pinol als Additional Voices (voice) |
 | Juan Pope als Additional Voices (voice) |
 | Al Rodrigo als Additional Voices (voice) |
 | Joseph Sanfelippo als Additional Voices (voice) |
 | Justin Shenkarow als Additional Voices (voice) |
 | Dennis Singletary als Additional Voices (voice) |
 | Warren Sroka als Additional Voices (voice) |
 | Melissa Sturm als Additional Voices (voice) |
 | Holly Walker als Additional Voices (voice) |
 | Jason Linere-White als Additional Voices (voice) |
 | Ruth Zalduondo als Additional Voices (voice) |
| Stan Lee | Executive Producer |
| Stan Lee | Characters |
| Stan Lee | Thanks |
| Stan Lee | In Memory Of |
| Steve Ditko | Characters |
| Steve Ditko | Thanks |
| Steve Ditko | In Memory Of |
| Avi Arad | Producer |
| Mary Hidalgo | Casting |
| Benjamin L. Cook | Sound Effects Editor |
| Cody Cameron | Story Artist |
| Tony Lamberti | Sound Re-Recording Mixer |
| Christina Steinberg | Producer |
| Sharon Bridgeman | Story Artist |
| Caitlin McKenna | ADR Voice Casting |
| Rodney Rothman | Screenplay |
| Rodney Rothman | Director |
| Robh Ruppel | Visual Development |
| Matt Landon | Editor |
| Phil Lord | Screenplay |
| Phil Lord | Producer |
| Phil Lord | Story |
| John Puglisi | Story Artist |
| Peter Ramsey | Director |
| Christopher Miller | Producer |
| Daniel Pemberton | Original Music Composer |
| James C.J. Williams | Head of Layout |
| Bob Persichetti | Director |
| Paul Lasaine | Visual Development |
| Robert Fisher Jr. | Editor |
| Justin K. Thompson | Production Design |
| Gary A. Hecker | Foley Artist |
| Arthur D. Noda | Editor |
| Michel Gagné | Visual Development |
| Stephan Franck | Story Artist |
| Randy Singer | Foley Mixer |
| Vivek 'Vic' Sharma | Associate Editor |
| Donald Flick | Sound Effects Editor |
| Rick Owens | Foley Artist |
| Michael J. Broomberg | Foley Artist |
| Michael Semanick | Sound Re-Recording Mixer |
| Brian Michael Bendis | Executive Producer |
| Christopher S. Aud | Sound Effects Editor |
| David Werntz | Sound Effects Editor |
| Patrick Ballin | Assistant Editor |
| Michael A. Reagan | Sound Effects Editor |
| Curt Schulkey | Supervising Sound Editor |
| Geoffrey G. Rubay | Supervising Sound Editor |
| James Morioka | Dialogue Editor |
| Amy Pascal | Producer |
| John Pospisil | Sound Designer |
| Agustín Ross Beraldi | Animation |
| Heiko von Drengenberg | Story Artist |
| Scott Armstrong | Layout |
| Andy Bialk | Visual Development |
| John Bermudes | Layout |
| Guillaume Roux | Animation |
| Eddie Prickett | Animation |
| Toby Pedersen | Animation |
| Joseph Izzo | Production Manager |
| Seung Hoo Ihm | Animation |
| Atsuo Fujiwara | Animation |
| Julius Kwan | Animation |
| Philip Rudolph | Supervising Animator |
| Juan Miguel Vadell | Animation |
| Mario Richard | Animation |
| Shiyoon Kim | Character Designer |
| Nicholas St. Clair | Animation |
| Tom Cohen | Thanks |
| Noëlle Triaureau | Visual Development |
| Mark Ackland | Story Artist |
| Ramón de la Cuesta | Animation |
| Pedro de la Llave | Animation |
| Kier Lehman | Music Supervisor |
| Katie Greathouse | Music Editor |
| Ando Johnson | Sound Effects Editor |
| Jesse Andrewartha | Software Engineer |
| Atsushi Sato | Animation |
| Matthew E. Taylor | Dialogue Editor |
| Alberto Mielgo | Other |
| Andrea Blasich | Character Designer |
| Denise Koyama | Story Artist |
| Carolyn Vale | Animation |
| Craig Kellman | Character Designer |
| Anibis Lockward | Layout |
| Dean Gordon | Art Direction |
| Jerry Loveland | Visual Development |
| Craig Mullins | Visual Development |
| Rich Turner | Head of Layout |
| Paula Assadourian | Story Artist |
| Riccardo Durante | Story Artist |
| Jennifer Dolce | Associate Editor |
| Laide Agunbiade | Lighting Artist |
| Laide Agunbiade | Compositing Artist |
| Miguel Jiron | Animation Supervisor |
| Miguel Jiron | Story Artist |
| Ryan Doell | Animation Coordinator |
| Michael Muir | CG Supervisor |
| Fraser Page | Animation |
| Will Allegra | Executive Producer |
| Raffaello Vecchione | Visual Development |
| Andy Sisul | Sound Effects Editor |
| David Gimenez | Animation |
| Juan Alejandro Gomez Acosta | Animation |
| Theresa Bentz | Co-Producer |
| Ernie Rinard | Visual Development |
| Hyesook Kim | Animation |
| Ali Pournassari | Animation |
| Hiroya Sonoda | Animation |
| Gregory Verreault | Animation |
| Jeffrey M. Thompson | Visual Development |
| Neil Campbell Ross | Visual Development |
| Christian Hejnal | Co-Producer |
| Yoriaki Mochizuki | Story Artist |
| Xinling Chen | Software Engineer |
| Hanung Lee | Animation |
| Roman Llanos | Animation |
| Nathan Dillow | Animation |
| Ryan Sivley | Animation |
| Matt Kummer | Animation |
| Tony Siruno | Character Designer |
| Gabe Hordos | Visual Development |
| Bailey Brent | Animation |
| Chloé Proulx | Animation |
| Julie Bernier Gosselin | Supervising Animator |
| Donald Chan | Animation |
| Joan Marc Fuentes Iglesias | Animation |
| Andrew Perez | Animation |
| Ryan Savas | Story Artist |
| Nideep Varghese | Animation |
| Chin Ko | Visual Development |
| Alec Rubay | Foley Supervisor |
| Jimmy Wu | Animation |
| Maritza Louis | Modeling |
| Yuchung Peter Chan | Visual Development |
| David Bleich | Visual Development |
| Darren Lurie | Digital Effects Supervisor |
| Dave Blais | Supervising Animator |
| Fernando Franco | Animation |
| Robin George | Supervising Animator |
| Andrew Hudec | Animation |
| José Más Huertas | Animation |
| Kevin Jackson | Animation |
| Bobby Taylor | Animation |
| Benjamin Aguillon | CG Supervisor |
| Joshua Beveridge | Head of Animation |
| Seonna Hong | Visual Development |
| Danny Dimian | Visual Effects Supervisor |
| Jeff Panko | Supervising Animator |
| Derek Esparza | Supervising Animator |
| Jason Petrocelli | Animation |
| Yashar Kassai | Visual Development |
| Eric Chou | Animation |
| Mark Tan | Animation |
| Eddy Okba | Animation |
| Eike Hoppmann | Animation |
| Martin Campos Amoros | Animation |
| Demetrios Tzamaras | Art Department Coordinator |
| Andreea Jebelean | Animation |
| Kevin Herron | Animation |
| Luiza Alaniz | Animation |
| Charles Abou Aad | CG Supervisor |
| Matt Hausman | CG Supervisor |
| Libby Thomas Dickey | Casting Associate |
| Stein Løtveit | Animation |
| Eddie Chew | Animation |
| James R. Ward | Animation |
| Zac Overcash | Animation |
| Pawel Grochola | Effects Supervisor |
| Jason Greenblum | CG Supervisor |
| Michael Kimmel | Animation |
| Barbaros Sarisozen | Modeling |
| Ben Foskett | Orchestrator |
| Q-Hyun Kim | Animation |
| Orrie Fung | Modeling |
| Michael Trikosko | Animation |
| Patrick O'Keefe | Art Direction |
| Rebecca Karch Tomlinson | Thanks |
| Takuro Togo | Animation |
| Leandro Martins | Animation |
| Aleksandar Kolev | Animation |
| Nick Kondo | Animation |
| Federico Abib | Animation |
| Josh Adler | Animation |
| Kiran Jay Babla | Animation |
| Chad Belteau | Rigging Supervisor |
| Simone Brundisini | Animation |
| Eva Bruschi | Story Artist |
| Daniel Caylor | Animation |
| Byung Joo Choi | Animation |
| Juan Couto | Animation |
| Jeremy Cummins | Animation |
| Sérgio Dias | Animation |
| Chad Ellis | Animation |
| Leon Anthony Enriquez | Modeling |
| Bianca Gee | Animation |
| Meghana Gupte | Animation |
| David Han | Animation |
| Laura H. Han | Animation |
| Derek Henriques | Animation |
| Nathan Hibberd | Animation |
| Alfredo Hisa | Layout |
| Min Hong | Supervising Animator |
| Michael Lee Huerto | Animation |
| David Jaraiz | Matte Painter |
| Steven R.V. Johnson | Animation |
| Thanawat Khantrum | Animation |
| Arem Kim | Layout |
| Kentaro Komiya | Animation |
| André Lacroix | Animation |
| Daniel Laczkowski | Layout |
| Tarun Lak | Animation |
| Letia Lewis | Layout |
| Fraser Littlejohn | Animation |
| Jose Luis Llado Porquer | Animation |
| Andres Bedate Martin | Animation |
| David Morehead | Head of Layout |
| Vi-Dieu Nguyen | Story Artist |
| Nicholas Nøstbakken | Animation |
| Ryan O'Reilly | Animation |
| Jihyun Park | Story Artist |
| Marshall Peterman | Animation |
| Cynthia Potvin | Animation Coordinator |
| Dylan Reid | Supervising Animator |
| Samuel Arturo Rico | Animation |
| Joakim Riedinger | Animation |
| Daniel A. Rodriguez | Animation |
| Owen Rohu | Matte Painter |
| Humberto Francisco Rosa | Supervising Animator |
| Rik Schutte | Animation |
| Martin Sen | Animation |
| Tatsuyuki Shimada | Animation |
| Harrison Smith | Animation |
| Chris Su | Animation |
| Jean-Yves Teillet | Lighting Artist |
| Jean-Yves Teillet | Compositing Artist |
| Miles Thompson | Story Artist |
| Ryo Wakasugi | Animation |
| Daniel Waters | Animation |
| Lisa Bechard | Digital Producer |
| Trey Roane | Animation |
| Sarah D. Cole | First Assistant Editor |
| Andrew Skeet | Orchestrator |
| Andrew Skeet | Conductor |
| Sam Verschraegen | Animation |
| Jessica E. Smialek | Post Production Supervisor |
| Stephanie Wilkie | Production Coordinator |
| Andrew Leviton | Associate Editor |
| Kristin Müller | Animation |
| Tom Davis | Animation |
| Meghan Malloy | Production Consultant |
| Larry Weiss | CG Supervisor |
| Chelsea Gordon-Ratzlaff | Animation |
| Kip Smedley | Sound Effects Editor |
| Nathan W Klein | Orchestrator |
| Brittany Myers | Character Designer |
| Benjamin Hendricks | CG Supervisor |
| Darren Quah | Matte Painter |
| Ian McQue | Thanks |
| Wendell Dalit | Visual Development |
| Talar Boyajian | Finance |
| Andres de los Rios | Animation |
| Omar Smith | Character Designer |
| Evelyn Gunawan | Animation |
| Jane Wu | Story Artist |
| Emma Shih | Animation |
| Eric Fineman | Thanks |
| Ben Lusthaus | Thanks |
| Andrew Sweet | Thanks |
| Adam Sarophim | Animation |
| Nicolas Gillet | Animation |
| Christopher Sanchez | Animation |
| Joseph Feinsilver | Visual Development |
| Zac Retz | Visual Development |
| Kyle Fukuto | Script Coordinator |
| Florent Auguy | Character Designer |
| Denis Bodart | Character Designer |
| Sei Riondet | Character Designer |
| Jesús Alonso Iglesias | Character Designer |
| Bret Allen | Assistant Editor |
| Daniel Evans | Editorial Coordinator |
| Ian Farnsworth | Effects Supervisor |
| Ozan Basaldi | Animation |
| Kitty Fung | Animation |
| Paulo Lombardi | Animation |
| Renato Dos Santos Sena | Animation |
| Stephanie Parker | Animation |
| Joshua Kalinowski | Visual Development |
| Naveen Selvanathan | Visual Development |
| Yuhki Demers | Visual Development |
| Bastien Grivet | Visual Development |
| Andrew Hartel | Visual Development |
| Tony Ianiro | Visual Development |
| Vaughan Ling | Visual Development |
| Mike Kurinsky | Visual Development |
| Nobuteru Sasagawa | Visual Development |
| Jessica Rossier | Visual Development |
| Yun Ling | Visual Development |
| Brittany Shively | Visual Development |
| Mike Winkelman | Visual Development |
| Joe Darko | Animation |
| Nicholas Georgeou | Animation |
| Earl Brawley | Animation |
| Nir Avital | Animation |
| Emma Cartwright | Animation |
| Daniel Hernandez Leyva | Animation |
| Sangyeong Jeong | Animation |
| Soh-I Jeong | Animation |
| Sejin Lee | Animation |
| Sophia Seung Hee Lee | Animation |
| Nico Mendes | Animation |
| Alex Olea | Animation |
| Guilherme Paiva | Animation |
| Rafael Polanczyk | Animation |
| Sinu Raghavan | Animation |
| Cody Schiebelbein | Animation |
| Woo Youp Shim | Animation |
| Jia Loon Sim | Animation |
| Eulrang Song | Animation |
| Mikey Wong | Animation |
| Juan Diego Zapata | Animation |
| Keenan Goncalves | Animation |
| Kilian Aulenbacher | Matte Painter |
| Andrew Cunningham | Matte Painter |
| Oliver Bubard | Matte Painter |
| Robert Go | Matte Painter |
| David Jaraiz Sanchez | Matte Painter |
| Guillem Rovira Vigas | Matte Painter |
| Alyssa Zarate | Matte Painter |
| Steven Edwards | Production Coordinator |
| Renee Mondor | Production Coordinator |
| Naomi Sider | Production Coordinator |
| Haley Flaniken | Finance |
| Ola Mota | Finance |
| Jennifer Jejo Sleeper | Finance |
| Dominik Malone | Production Assistant |
| Christian Mejia-Acosta | Production Assistant |
| Zara Pokrandt | Production Assistant |
| Kylee A. Taylor | Production Assistant |
| Dale W. Jones | Production Assistant |
| Sean Tadlock | Production Assistant |
| Ryan Gaffney | Story Artist |
| Katie Smith | Story Artist |
| Monica Kelly | Post Production Supervisor |
| Jabari Cofer | Animation |
| Guillaume Belanger | Animation |
| Simon Zizan Black | Animation |
| Patrick Blazin | Animation |
| Martin Esnaola Scotto | Animation |
| Richard Alexander Forero Domínguez | Animation |
| Joe Gearing | Animation |
| Marcelo Gravina Zanin | Animation |
| Sofia Grigoreva | Animation |
| Rhys Hanan | Animation |
| David Hernandez | Animation |
| Chus Herrero | Animation |
| Yuko Ikeda | Animation |
| Tristan Jeanbourquin | Animation |
| Inhwan Kim | Animation |
| Jenny Yan-Yee Kong | Animation |
| Chaeyeon Lee | Animation |
| Minseok Lee | Animation |
| Evan McGowan | Animation |
| Patrick A. Mueller | Animation |
| Steve Oh | Animation |
| Sam Ortiz | Animation |
| Sean Ari Peterson | Animation |
| Jacobo Pineiro Rumbo | Animation |
| Victor G. Reano | Animation |
| Nathaniel Saunders | Animation |
| Jason Spilchak | Animation |
| Nick Starcevic | Animation |
| Joseph Taylor | Animation |
| Darragh White | Animation |
| Jeeyun Won | Animation |
| Peter Chen | Modeling |
| Marcus Ng | Modeling |
| Booby Yoonsung Jeong | Modeling |
| Unsol Parl | Modeling |
| Sung-Hum Lim | Modeling |
| Josef Dylan Swift | Modeling |
| Adrien Montero | Modeling |
| Mike Sungjoon Hong | Modeling |
| Pil Gyu Cheng | Modeling |
| Joseph Oyem | Modeling |
| Shinya Ishii | Modeling |
| Guillaume Fuentes | Modeling |
| Yeaji Jessie Lee | Modeling |
| Richard Gylser | Modeling |
| David Vidal Pedroza | Modeling |
| Yu-Cheng Huang | Modeling |
| Wendy Chan | Animation Coordinator |
| Emily Delion | Animation Coordinator |
| Vivasean Venkat | Animation Coordinator |
| Sagar Alodiya | Lighting Artist |
| Sagar Alodiya | Compositing Artist |
