 | Tobey Maguire als Spider-Man / Peter Parker |
 | Kirsten Dunst als Mary Jane Watson |
 | James Franco als Harry Osborn |
 | Alfred Molina als Doc Ock / Otto Octavius |
 | Rosemary Harris als May Parker |
 | J.K. Simmons als J. Jonah Jameson |
 | Donna Murphy als Rosalie Octavius |
 | Daniel Gillies als John Jameson |
 | Dylan Baker als Dr. Curt Connors |
 | Bill Nunn als Joseph 'Robbie' Robertson |
 | Vanessa Ferlito als Louise |
 | Aasif Mandvi als Mr. Aziz |
 | Willem Dafoe als Green Goblin / Norman Osborn |
 | Cliff Robertson als Ben Parker |
 | Ted Raimi als Hoffman |
 | Elizabeth Banks als Miss Brant |
 | Bruce Campbell als Snooty Usher |
 | Gregg Edelman als Dr. Davis |
 | Elya Baskin als Mr. Ditkovitch |
 | Mageina Tovah als Ursula |
 | Daniel Dae Kim als Raymond |
 | Hal Sparks als Elevator Passenger |
 | Joel McHale als Mr. Jacks |
 | Stan Lee als Man Dodging Debris |
 | Kelly Connell als Dr. Isaacs |
 | Brent Briscoe als Garbage Man |
 | Emily Deschanel als Receptionist |
 | Jason Fiore-Ortiz als Henry Jackson |
 | Scott Spiegel als Man on Balcony |
 | Andy Bale als OsCorp Executive |
 | Christine Estabrook als Mrs. Jameson |
 | Molly Cheek als Society Woman |
 | John Paxton als Houseman |
 | Joy Bryant als Woman at Web |
 | Joanne Baron als Skeptical Scientist |
 | Peter McRobbie als OsCorp Representative |
 | Timothy Jerome als Injured Scientist |
 | Taylor Gilbert als Mrs. Watson |
 | Peter Vouras als Stage Manager |
 | Donnell Rawlings als Pizza "Heist" Witness |
 | Zachry Rogers als Boy saved by Spider-Man |
| Ella Rogers als Girl saved by Spider-Man |
 | Louis Lombardi als Poker Player |
 | Marc John Jefferies als Amazed Kid |
 | Roshon Fegan als Amazed Kid |
 | Brendan Patrick Connor als Theater Traffic Cop |
 | Reed Diamond als 'Algernon' |
 | Dan Callahan als 'Jack' |
 | Elyse Dinh als Violinist |
 | John Landis als Doctor |
 | Tim Storms als Chainsaw Doctor |
 | Susie Park als Clawing Nurse |
 | Patricia M. Peters als Screaming Nurse |
 | Michael Edward Thomas als Man at Fire |
 | Anne Betancourt als Woman at Fire |
| Venus Lam als Child in Burning Building |
 | Bill E. Rogers als Fireman |
 | Joe Virzi als Fireman |
| Tom Carey als Train Conductor |
| Jopaul Van Epp als Boy with Mask |
| Weston Epp als Boy with Mask |
 | Peter Allas als Train Passenger |
 | Brianna Brown als Train Passenger |
 | Bill Calvert als Train Passenger |
 | Tony Campisi als Train Passenger |
 | Joey Diaz als Train Passenger |
 | Chloe Dykstra als Train Passenger |
| Simone Gordon als Train Passenger |
 | Dan Hicks als Train Passenger |
 | Julia Max als Train Passenger |
| Savannah Pope als Train Passenger |
 | Timothy Patrick Quill als Train Passenger |
 | Jill Sayre als Train Passenger |
 | Rickey G. Williams als Train Passenger |
 | Michael Arthur als NYPD Officer (uncredited) |
| Frank Bonsangue als Pizza Man (uncredited) |
 | Cindy Cheung als Chinese Daughter (uncredited) |
 | Phil LaMarr als Train Passenger (uncredited) |
 | Calvin Dean als Boomer (uncredited) |
 | Andre M. Johnson als Fireman 2 (uncredited) |
 | Peter Cincotti als Piano Player in Planetarium (uncredited) |
 | Peyton List als Little Girl Playing on Steps (uncredited) |
 | Spencer List als Little Boy Playing on Steps (uncredited) |
 | Troy Metcalf als Blue Collar Guy (uncredited) |
| Scott Ross als Pedestrian (uncredited) |
 | Bonnie Somerville als Screaming Woman (uncredited) |
| Wesley Volcy als Columbia University Student (uncredited) |
 | Lou Volpe als Man at Web (uncredited) |
 | Garrett Warren als Bearded Doctor (uncredited) |
| Joseph M. Caracciolo als Priest at Mary Jane's Wedding (uncredited) |
| Sam Raimi | Director |
| Alvin Sargent | Screenplay |
| Avi Arad | Producer |
| Lorne Orleans | Producer |
| Laura Ziskin | Producer |
| Joseph M. Caracciolo Jr. | Executive Producer |
| Kevin Feige | Executive Producer |
| Stan Lee | Executive Producer |
| Danny Elfman | Original Music Composer |
| Bill Pope | Director of Photography |
| Bob Murawski | Editor |
| Dianne Crittenden | Casting |
| Neil Spisak | Production Design |
| Jay Hart | Set Decoration |
| James Acheson | Costume Design |
| Gary Jones | Costume Design |
| Bob Finley III | Gaffer |
| John Dillon | Art Department Manager |
| Grant Curtis | Co-Producer |
| Steve Ditko | Comic Book |
| Jeff Knipp | Art Direction |
| Scott P. Murphy | Art Direction |
| Steve Saklad | Art Direction |
| Thomas Valentine | Art Direction |
| Thomas P. Wilkins | Art Direction |
| Trish Almeida | Key Hair Stylist |
| Tricia Heine | Makeup Artist |
| Eric Heffron | Assistant Director |
| Dan Bradley | Stunt Coordinator |
| Hank Amos | Utility Stunts |
| Trudy Ramirez | Script Supervisor |
| Matt Blades | Grip |
| Gershon Hinkson | Assistant Editor |
| Bill Abbott | Music Editor |
| J.C. Brotherhood | Special Effects Supervisor |
| Eran Barnea | Visual Effects |
| Brinton Bryan | Set Production Assistant |
| Larry Hopkins | Sound Mixer |
| Chris Haynes | Driver |
| Joseph M. Caracciolo | Executive Producer |
| David Campbell | Orchestrator |
| Michael V. Nicolo | Casting Associate |
| Jason Hamer | Special Effects |
| Timothy Healy | Electrician |
| Theresa Greene | Art Department Assistant |
| Jan O'Connell | Art Department Coordinator |
| Cosmas A. Demetriou | Assistant Art Director |
| Jim Ondrejko | Construction Coordinator |
| Jason Bartolone | Construction Foreman |
| Wayne Shepherd | Leadman |
| Bill Garvey | Location Scout |
| Paulette Fox | Painter |
| James Carson | Production Illustrator |
| J. Bryan Holloway | Sculptor |
| C. Scott Baker | Set Designer |
| Vinson Jae | Standby Painter |
| Joseph V. Cicio | Camera Operator |
| Tim Arasheben | Camera Technician |
| Wayne Baker | First Assistant Camera |
| Al Cerullo | Helicopter Camera |
| Kenji Luster | Steadicam Operator |
| John Bramley | Still Photographer |
| Clare Hallworth | Assistant Costume Designer |
| Kelvin R. Trahan | Hair Department Head |
| Anita Lausevic | Hairstylist |
| Julie Hewett | Makeup Department Head |
| Debbie Lucas | Seamstress |
| Gretchen Gain | Set Costumer |
| Steve Bartek | Additional Music |
| Michael Kall | Carpenter |
| Daniel Eaton | CG Supervisor |
| Michael K. Reynolds | Chef |
| William Ramirez Jr. | Craft Service |
| Cyril O'Neil | Picture Car Coordinator |
| Nour Dardari | Production Controller |
| David Irvine | Production Intern |
| Heather Sharpe | Production Office Assistant |
| Douglas Cannon | Propmaker |
| Timothy Michael Cairns | Quality Control Supervisor |
| Robert Barnett | Scenic Artist |
| Dan Harder | Set Medic |
| Cottalango Loorthu | Software Engineer |
| Danny Cangemi | Special Effects Coordinator |
| Steven Clawson | Stand In |
| Hank Amos | Stunts |
| Christopher Wolf | Systems Administrators & Support |
| Don Poole | Transportation Co-Captain |
| John Orlebeck | Transportation Coordinator |
| Richard Baratta | Unit Production Manager |
| Jody Fedele | Visual Effects Editor |
| Jim Passon | Color Timer |
| Sean Valla | First Assistant Editor |
| Sidney Brisco | Lighting Technician |
| Frank Dorowsky | Rigging Gaffer |
| Laura Berning | Location Manager |
| Tamara Bally | Production Accountant |
| Susan Dukow | Production Coordinator |
| David J. Grant | Production Supervisor |
| Sandy O'Neill | Publicist |
| Todd Bassman | Boom Operator |
| Joseph Geisinger | Production Sound Mixer |
| Dennis S. Sands | Scoring Mixer |
| Paul N.J. Ottosson | Sound Designer |
| Scott G.G. Haller | Sound Editor |
| John M. Chalfant | Sound Effects Editor |
| Paul N.J. Ottosson | Supervising Sound Editor |
| Julie Adrianson-Neary | Digital Compositors |
| Carlye Archibeque | Visual Effects Coordinator |
| Raoul Bolognini | Visual Effects Producer |
| Greg Anderson | Visual Effects Supervisor |
| Alicia Kalvin | Studio Teachers |
| Carey Smith | Digital Producer |
| Noriko Watanabe | Hairstylist |
| Scott Getzinger | Assistant Property Master |
| Egan Gauntt | Set Decorating Coordinator |
| Adam Austin | Set Dresser |
| Kyle Cooper | Title Designer |
| Ulrike Lamster | Camera Loader |
| Daniel Pershing | Dolly Grip |
| Kelly R. Borisy | Key Grip |
| Ivory Stanton | Ager/Dyer |
| Bob Moore Jr. | Key Costumer |
| Elisa Marsh | Key Makeup Artist |
| Sara Auhagen | Tailor |
| Mary Jo 'MJ' McGrath | Wardrobe Supervisor |
| Christian Ristow | Animatronics Designer |
| Dion Lam Dik-On | Fight Choreographer |
| Jason Kay | Techno Crane Operator |
| Bac DeLorme | Second Assistant Director |
| Mo Henry | Negative Cutter |
| Jenifer Bonisteel | Assistant Production Coordinator |
| Kevin Flatow | Assistant Production Manager |
| Ulrika Akander | ADR Editor |
| George Doering | Musician |
| Susan Dudeck | Supervising ADR Editor |
| Susan Dudeck | Supervising Dialogue Editor |
| Vincent Truitner | CG Animator |
| Peter Giliberti | Lead Animator |
| Bob Mano | Mechanical Designer |
| Colin Drobnis | Visual Effects Technical Director |
| Ulrika Akander | Dialogue Editor |
| Alison Fisher | Dialogue Editor |
| Doug Harlocker | Property Master |
| Sean Landeros | Sound Recordist |
| Douglas J. Schulman | Sound Mixer |
| Stan Lee | Comic Book |
| John Dykstra | Visual Effects Designer |
| Todd Harris | First Assistant Sound Editor |
| Alethea McElroy | Additional Wardrobe Assistant |
| Tina Dowd | Costume Assistant |
| Lorraine Crossman | Costumer |
| Lynda Kyle Walker | Hair Assistant |
| Alexandra K. Browne | Additional Second Assistant Director |
| Jeff Shiffman | Second Second Assistant Director |
| Dan Bradley | Second Unit Director |
| Chris John Jones | Creative Director |
| François Audouy | Graphic Designer |
| Michael Scarola | Key Construction Grip |
| Thomas Moffitt | Set Painter |
| Jerry Bingham | Storyboard Artist |
| Aaron Scully | Storyboard Assistant |
| Howard London | ADR Mixer |
| Tricia Linklater | First Assistant Sound Editor |
| Nancy Parker | Foley Artist |
| Christopher Flick | Foley Supervisor |
| H. Barclay Aaris | Special Effects Technician |
| Chris Daniels | Stunt Double |
| James Bearb | Stunt Driver |
| Anette Haellmigk | Additional Director of Photography |
| Giselle Brewton | Assistant Camera |
| Kevin Flynn | Best Boy Grip |
| Mark McDevitt | Clapper Loader |
| Steve Robertson | Dolly Grip |
| Braden Belmonte | Second Assistant Camera |
| Anthony B. LaMolinara | Animation Director |
| Luis Grané | Senior Animator |
| Anthony Harris | Digital Color Timer |
| Michael Healey | Digital Colorist |
| Jean Chien | Assistant Location Manager |
| Alex Borys | Location Assistant |
| Pete Anthony | Conductor |
| Angela Athayde | Additional Production Assistant |
| Santena Jenaris King Clough | Assistant Accountant |
| Trilochan Naik | CG Artist |
| Andy Ryan | Chief Lighting Technician |
| Patti Gannon | Compositing Supervisor |
| Tony Hobbs | Extras Casting |
| Judy Pursley | First Assistant Accountant |
| Kristen Branan | Head of Production |
| Jason Rody | Key Production Assistant |
| James Boniece | Key Rigging Grip |
| Joseph M. Horrigan | Medical Consultant |
| Marilyn DeJesus | Payroll Accountant |
| Dave Ardinger | Production Assistant |
| Shannon Gaulding | Production Executive |
| Kathryn Malone | Production Secretary |
| Basia Emano | Researcher |
| Michael Backes | Scientific Consultant |
| Carlo Pratto | Second Assistant Accountant |
| Grant Anderson | Sequence Lead |
| Kirk Bloomberg | Additional Second Assistant Camera |
| John Dykstra | Visual Effects Supervisor |
| Scott Stokdyk | Visual Effects Supervisor |
| John Frazier | Special Effects Supervisor |
| Kevin O'Connell | Sound Re-Recording Mixer |
| Greg P. Russell | Sound Re-Recording Mixer |
| Jeffrey J. Haboush | Sound Re-Recording Mixer |
| Joseph S. Alfieri | Construction Coordinator |
| Brad Martin | Stunt Double |
| Brad Martin | Stunts |
| Cinzia Angelini | Senior Animator |
| Gary Archer | Prosthetics |
| Steve Bartek | Orchestrator |
| Armen Ksajikian | Musician |
| Lisa Hannan | Sound Effects Editor |
| Chad Stahelski | Stunts |
| Diana R. Lupo | Stunts |
| Joanne Lamstein | Stunts |
| Tad Griffith | Stunt Driver |
| Jill Brown | Stunts |
| Billy Taylor | Production Assistant |
| Alexa Sheehan | Production Assistant |
| Alfred Gough | Screenstory |
| Miles Millar | Screenstory |
| Michael Chabon | Screenstory |
| Lisa Hoyle | Stunts |
| Cheryl Rusa | Utility Stunts |
