 | William Shatner als Admiral James T. Kirk |
 | Leonard Nimoy als Captain Spock |
 | DeForest Kelley als Dr. Leonard McCoy |
 | James Doohan als Montgomery Scott |
 | George Takei als Cmdr. Hikaru Sulu |
 | Walter Koenig als Cmdr. Pavel Chekov |
 | Nichelle Nichols als Cmdr. Uhura |
 | Ricardo Montalban als Khan Noonien Singh |
 | Kirstie Alley als Lt. Saavik |
 | Paul Winfield als Captain Clark Terrell |
 | Bibi Besch als Dr. Carol Marcus |
 | Merritt Butrick als Dr. David Marcus |
 | Judson Scott als Joachim |
 | Ike Eisenmann als Preston |
 | John Vargas als Jedda |
 | John Winston als Kyle |
 | Paul Kent als Beach |
| James Horner | Original Music Composer |
| Joseph R. Jennings | Production Design |
| Robert Fletcher | Costume Design |
| Nicholas Meyer | Director |
| Robert Sallin | Producer |
| Harve Bennett | Executive Producer |
| Harve Bennett | Story |
| Jack B. Sowards | Screenplay |
| Jack B. Sowards | Story |
| John Newton | Additional Soundtrack |
| Gayne Rescher | Director of Photography |
| Michael Minor | Art Direction |
| Charles Graffeo | Set Decoration |
| Mary V. Buck | Casting |
| William Paul Dornisch | Editor |
| Ralph Winter | Post Production Supervisor |
| Gilbert B. Combs | Stunts |
| Mike Washlake | Stunts |
| Allan Graf | Stunts |
| Ken Ralston | Visual Effects Supervisor |
| George P. Wilbur | Stunts |
| David J. Hudson | Sound Re-Recording Mixer |
| Mel Metcalfe | Sound Re-Recording Mixer |
| Dione Taylor | Hairstylist |
| Chuck Hicks | Stunts |
| Austen Jewell | Unit Production Manager |
| John Robotham | Stunts |
| Jim Burk | Stunts |
| Bill Couch | Stunt Coordinator |
| John Escobar | Stunts |
| Paula Moody | Stunts |
| Gary Combs | Stunts |
| Tom Morga | Stunts |
| Cecelia Hall | Supervising Sound Editor |
| George Watters II | Supervising Sound Editor |
| Tommy J. Huff | Stunts |
| Hubie Kerns Jr. | Stunts |
| Ray West | Sound Re-Recording Mixer |
| Eddy Donno | Stunts |
| Anthony Cecere | Stunts |
| Bruce Birmelin | Still Photographer |
| James R. Alexander | Sound Mixer |
| Douglas E. Wise | First Assistant Director |
| Steve Blalock | Stunts |
| William F. Phillips | Associate Producer |
| Jim Connors | Stunts |
| Bill Couch Jr. | Stunts |
| Janet Brady | Stunts |
| James Lee McCoy | Makeup Artist |
| Noyan Cosarer | Sound Re-Recording Mixer |
| Kym Washington Longino | Stunts |
| Jim Veilleux | Visual Effects Supervisor |
| Ernest Robinson | Stunts |
| Richard Espinoza | Second Assistant Director |
| Beth Nufer | Stunts |
| Mary Peters | Stunts |
| Werner Keppler | Makeup Artist |
| Fletcher Bryant | Stunts |
| Bob Dawson | Special Effects |
| Romolo Acquistapace | Gaffer |
| Ann Chatterton | Stunts |
| Martin Becker | Special Effects |
| Diane Carter | Stunts |
