 | Michael J. Fox als Stuart Little (voice) |
 | Melanie Griffith als Margalo (voice) |
 | Nathan Lane als Snowbell (voice) |
 | Geena Davis als Mrs. Little |
 | Hugh Laurie als Mr. Little |
 | Jonathan Lipnicki als George Little |
 | Steve Zahn als Monty (voice) |
 | James Woods als Falcon (voice) |
 | Maria Bamford als Teacher |
 | Anna Hoelck als Martha Little |
| Ashley Hoelck als Martha Little |
| Kevin Olson als Irwin |
 | Marc John Jefferies als Will |
| Michael C. Fuchs als Mark |
 | Frank Aquilino als Onlooker |
 | Angelo Massagli als Wallace |
 | Brad Garrett als Plumber |
 | Conan McCarty als Referee |
 | Jim Doughan als Soccerball Coach |
 | Rachael Harris als Additional Voices (voice) |
| Rob Minkoff | Director |
| Douglas Wick | Producer |
| Alan Silvestri | Original Music Composer |
| Steven Poster | Director of Photography |
| Priscilla Nedd-Friendly | Editor |
| Craig R. Maras | Animation |
| Angie Jones | Animation |
| Thor Freudenthal | Conceptual Design |
| Rob Minkoff | Executive Producer |
| Tony R. Medina | Propmaker |
| Douglas Wick | Story |
| Bruce Joel Rubin | Story |
| Bruce Joel Rubin | Screenplay |
| E.B. White | Characters |
| Jerome Chen | Visual Effects Supervisor |
| Lucy Fisher | Producer |
| Steve Waterman | Executive Producer |
| Jeff Franklin | Executive Producer |
| Jason Clark | Executive Producer |
| Bill Brzeski | Production Design |
| Gail Lyon | Executive Producer |
| Mona May | Costume Design |
| Francine Maisler | Casting |
| Bonnie Greenberg | Music Supervisor |
| Danny Aiello III | Stunt Coordinator |
| Boone Narr | Stunt Coordinator |
| Michelle Murdocca | Associate Producer |
| Michelle Murdocca | Visual Effects Producer |
| Tony Bancroft | Animation Supervisor |
| Eric Armstrong | Animation Supervisor |
| Shepherd Frankel | Art Direction |
| Rachel Shane | Associate Producer |
| Lisa K. Sessions | Set Decoration |
| Bonita DeHaven | Makeup Supervisor |
| Tammy Ashmore | Makeup Artist |
| Robert Hallowell II | Hair Supervisor |
| Mary Guerrero | Hairstylist |
| Mark Vargo | Second Unit Director |
| Mark Herman | Visual Effects Editor |
| David A. Whittaker | Supervising Sound Editor |
| Donald Flick | Sound Designer |
| Kenneth Karman | Music Editor |
| Jay Redd | Digital Effects Supervisor |
| George Denes Suhayda | Visual Effects Art Director |
| Bill Westenhofer | Visual Effects Supervisor |
| Dan Foster | Visual Effects Producer |
| Christopher Sjoholm | Digital Effects Supervisor |
| John Cenatiempo | Stunts |
