 | Andrew Garfield als Peter Parker / Spider-Man |
 | Emma Stone als Gwen Stacy |
 | Rhys Ifans als Dr. Curt Connors / The Lizard |
 | Martin Sheen als Uncle Ben |
 | Sally Field als Aunt May |
 | Denis Leary als Captain Stacy |
 | Irrfan Khan als Rajit Ratha |
 | Max Charles als Peter Parker (Age 4) |
 | Campbell Scott als Richard Parker |
 | Embeth Davidtz als Mary Parker |
 | Chris Zylka als Flash Thompson |
 | C. Thomas Howell als Jack's Father |
 | Jake Keiffer als Jack |
 | Hannah Marks als Missy Kallenback |
 | Kari Coleman als Helen Stacy |
| Michael Barra als Store Clerk |
 | Leif Gantvoort als Cash Register Thief |
 | Andy Pessoa als Gordon |
 | Kelsey Asbille als Sally Avril |
 | Kevin McCorkle als Mr. Cramer |
 | Andy Gladbach als Physics Nerd |
 | Ring Hendricks-Tellefsen als Physics Nerd |
 | Barbara Eve Harris als Miss Ritter |
 | Stan Lee als School Librarian |
 | Danielle Burgio als Nicky's Girlfriend |
 | Tom Waite als Nicky |
 | Keith Campbell als Car Thief |
 | Steve DeCastro als Car Thief Cop |
 | Jill Flint als Receptionist |
 | Mark Daugherty als OsCorp Intern |
 | Milton González als Rodrigo Guevara |
 | Skyler Gisondo als Howard Stacy |
 | Charlie DePew als Philip Stacy |
 | Jacob Rodier als Simon Stacy |
 | Vincent Laresca als Construction Worker |
 | Damien Lemon als Taxi Driver |
 | Ty Upshaw als Police Officer with Sketch |
 | James Chen als Police Officer |
 | Alexander Bedria als Officer (SWAT) |
 | Tia Texada als Sheila (Subway) |
 | Jay Caputo als Subway Guy |
 | John Burke als Newscaster (News Chopper) |
 | Terry Bozeman als Principal |
 | Jennifer Lyons als Second Girl (Subway) |
 | Michael Massee als Man in the Shadows |
 | Amber Stevens West als Ariel |
 | Max Bogner als Scientist (uncredited) |
 | Ethan Cohn als Lab Technician (uncredited) |
 | Miles Elliot als Billy Connors (uncredited) |
 | Miranda LaDawn Hill als Cheerleader (uncredited) |
 | Amanda MacDonald als Goth Girl (uncredited) |
 | Maury Morgan als Tina (uncredited) |
 | Michael Papajohn als Alfie (uncredited) |
| Stan Lee | Executive Producer |
| Laura Ziskin | Producer |
| Ve Neill | Makeup Department Head |
| Matt Tolmach | Producer |
| Avi Arad | Producer |
| Marc Webb | Director |
| James Horner | Original Music Composer |
| John Schwartzman | Director of Photography |
| Michael Grillo | Executive Producer |
| James Vanderbilt | Screenplay |
| Alan Edward Bell | Editor |
| Pietro Scalia | Editor |
| Kym Barrett | Costume Design |
| Kevin Feige | Executive Producer |
| Vincent Schicchi | Makeup Department Head |
| Steve Ditko | Characters |
| Stan Lee | Characters |
| Alvin Sargent | Screenplay |
| Steve Kloves | Screenplay |
| James Vanderbilt | Story |
| Francine Maisler | Casting |
| Leslie A. Pope | Set Decoration |
| Lynda Foote | Costume Supervisor |
| Page Buckner | Art Direction |
| Michael E. Goldman | Art Direction |
| Susan J. Wright | Costume Supervisor |
| Suzan Wexler | Art Direction |
| J. Michael Riva | Production Design |
| Eric Sundahl | Set Designer |
| Shannon Mills | Supervising Sound Editor |
| Cuitlahuac Morales Velazquez | Sculptor |
| Abe Costanza | Scenic Artist |
| David F. Klassen | Supervising Art Director |
| David Giammarco | Sound Re-Recording Mixer |
| Jerome Chen | Visual Effects Supervisor |
| Kevin Crandell | Visual Effects Editor |
| John Clinton | Visual Effects Producer |
| Paul Sonski | Assistant Art Director |
| Andrew Birdzell | Set Designer |
| John Berri | Visual Effects Editor |
| Hetal Jain | Visual Effects Producer |
| David R. Christensen | Gaffer |
| Ian Fox | Camera Operator |
| Jaimie Trueblood | Still Photographer |
| Mustapha Mimis | Set Costumer |
| Bob Moore Jr. | Set Costumer |
| Elizabeth Frank | Set Costumer |
| Terry Bradley | Visual Effects Supervisor |
| Andy Armstrong | Stunt Coordinator |
| David Luckenbach | Steadicam Operator |
| Malik Summers | Set Costumer |
| Robin Harlan | Foley |
| Michael Nelson | Camera Operator |
| Joe E. Rand | Music Editor |
| Trisha Burton | Script Supervisor |
| Noelle King | Set Designer |
| Ashley Beck | Visual Effects Supervisor |
| David Luckenbach | Camera Operator |
| Michelle Collier | Art Department Coordinator |
| Eric Hugunin | Assistant Art Director |
| Nancy A. King | Art Department Coordinator |
| William J. Law III | Set Designer |
| Shannon Mills | Sound Designer |
| Addison Teague | Supervising Sound Editor |
| James Armstrong | Stunt Coordinator |
| Joel Marrow | Transportation Coordinator |
| Russell R. Anderson | Leadman |
| Patrick Ballin | Visual Effects Editor |
| Julia Neighly | Visual Effects Producer |
| Barbara McDermott | Music Editor |
| Paul Massey | Sound Re-Recording Mixer |
| Lindsey Zamplas | Visual Effects Producer |
| Julie Orosz | Visual Effects Producer |
| Valentina Aulisi | Set Costumer |
| Elizabeth Himelstein | Dialect Coach |
| Ernie Avila | Set Designer |
| Thomas Machan | Set Designer |
| Sarah Monat | Foley |
| Sébastien Moreau | Visual Effects Supervisor |
| Peter Mercurio | Camera Operator |
| Deb Adair | Sound Re-Recording Mixer |
| Jennifer Silver | Visual Effects Producer |
| Jim Henrikson | Music Editor |
| Matt Daly | Visual Effects Producer |
| Paul Corricelli | Set Costumer |
| Addison Teague | Sound Designer |
| Mark Herman | Visual Effects Editor |
| David Fernandez | Set Costumer |
| Julie Shack | Set Costumer |
| Joseph A. Alfieri Jr. | Construction Coordinator |
| Joe Farrell | Visual Effects Supervisor |
| Russell Engels | Gaffer |
| Hugo Dominguez | VFX Artist |
| Earl A. Hibbert | Visual Effects |
| Joel Foster | Animation |
| Mike Dharney | Animation |
| Aaron Williams | VFX Artist |
| Elizabeth McClurg | Compositors |
| Sascha Wolf | Visual Effects Editor |
| Dean Andre | Sound mixer |
| Bill Corso | Makeup Department Head |
| VFx Star | Visual Effects |
| Jatinder Singh Manhas | Visual Effects |
| Robert Edwards | ADR Mixer |
| Robert Troy | Dialogue Editor |
| Andrew M. Siegel | Property Master |
| Richard Mosier | First Assistant Camera |
| Rick Harris | Key Rigging Grip |
| Jason Apperson | Lighting Technician |
| Joshua Davis | Lighting Technician |
| Jared Greenstein | Electrician |
| Stuart Kirschner | Electrician |
| James Fitzpatrick | Electrician |
| Matthew Kistenmacher | Electrician |
| Maricella Ramirez | First Assistant Camera |
| William Moore | Generator Operator |
| Daniel Cotroneo | Lighting Technician |
| Peter M. Smith | Lighting Technician |
| Jerame Maas | Electrician |
| Beth O'Brien | Electrician |
| Dobb Schreiber | Electrician |
| Trish Herremans | Electrician |
| Nick Budding | Lighting Technician |
| Dave Anderson | Electrician |
| James Mahoney | Electrician |
| Michael Alexonis | Key Grip |
| Tony Hibbard | Lighting Technician |
| Jordan Johnson | Lighting Technician |
| Greg Baldi | Camera Operator |
| Jaim O'Neil | Electrician |
| Jon Wolowec | Electrician |
| Mark Santoni | First Assistant Camera |
| Ted Goodwin | Electrician |
| Michael Arisohn | Electrician |
| Jason Cortazzo | Libra Head Technician |
| Scott Barnes | Lighting Programmer |
| William Self | Lighting Technician |
| Christopher A. Schenck | Camera Operator |
| Justin Situ | Electrician |
| John Leigh | Electrician |
| Billy Kerwick | Key Rigging Grip |
| Thomas DeRose | Lighting Technician |
| Diana Matos | Camera Loader |
| Christopher Dorowsky | Electrician |
| Les T. Tomita | Key Grip |
| Diana Ulzheimer | Camera Loader |
| Ralph Del Castillo | Dolly Grip |
| Carlos Baker | Electrician |
| James J. Ferris | Electrician |
| Alan R. Disler | First Assistant Camera |
| Darrin DeLoach | First Assistant Camera |
| Sean Higgins | Lighting Technician |
| Jim McComas | Electrician |
| Mark Schwentner | Electrician |
| Joshua Solson | Electrician |
| Shane D. Kelly | Gaffer |
| Richard Rasmussen | Lighting Programmer |
| Kurt E. Soderling | Aerial Director of Photography |
| Tommy Tieche | First Assistant Camera |
| Charles Sparks | Electrician |
| Nathan Dean Fetzer | Lighting Technician |
| Zoran Milosavljevic | Electrician |
| Damon Marcellino | Lighting Technician |
| Miguel López-Castillo | Art Direction |
| John Connell | Camera Operator |
| Jose Aguirre | Electrician |
| Jesse Gregson | Lighting Technician |
| Sean Ruggeri | Red Technician |
| Justin Corbett | Rigging Grip |
| Mike Tenerelli | Rigging Grip |
| Thomas D. Lairson Jr. | Second Assistant Camera |
| Gaylen Nebeker | Video Assist Operator |
| Gerald Quist | Key Makeup Artist |
| Aimee Macabeo | Special Effects Makeup Artist |
| Doug Dalisera | Rigging Gaffer |
| Fernando Barrios | Rigging Grip |
| Angel De Angelis | Hair Department Head |
| Yvette Stone | Key Hair Stylist |
| Tracy Estes | Lighting Technician |
| Dustin VonLossberg | Rigging Grip |
| Connie Grayson Criswell | Contact Lens Technician |
| Mike Mekash | Special Effects Makeup Artist |
| Nate Heartt | Red Technician |
| Cary Caraway | Techno Crane Operator |
| John Betancourt | Ultimate Arm Operator |
| Brian D'Haem | Lighting Technician |
| Michelle Ceglia | Hairstylist |
| Renate Leuschner | Wigmaker |
| Clayton Fowler | Rigging Grip |
| Danny Nallan | Rigging Grip |
| Barbara Cantu | Hair Department Head |
| Nikoletta Skarlatos | Key Makeup Artist |
| Jed Dornoff | Makeup Artist |
| Corinna Woodcock | Makeup Artist |
| Lindsay MacGowan | Prosthetic Supervisor |
| Jeremy Griffiths | Rigging Grip |
| Kathrine Gordon | Hair Department Head |
| Teressa Hill | Key Hair Stylist |
| Vincent Schicchi | Key Makeup Artist |
| Timothy Jipping | Rigging Grip |
| Robin Bursey | Second Assistant Camera |
| Noah Bishop | Techno Crane Operator |
| Paul Storino | Rigging Grip |
| R. Cory McCutcheon | Hairstylist |
| Joann Onorio | Hairstylist |
| Robert Wilson | Hairstylist |
| Parker Shultz | Rigging Grip |
| Kris Evans | Makeup Artist |
| Tricia Sawyer | Makeup Artist |
| Brian Bishop | Rigging Grip |
| Henry Tirl | Steadicam Operator |
| Shane Mahan | Prosthetic Supervisor |
| Andrew Clarke | Lighting Technician |
| Frank Dorowsky | Rigging Gaffer |
| Christopher Allen Nelson | Special Effects Makeup Artist |
| Richard Graves | First Assistant Director |
| Sebastian Lauer | CG Artist |
| Andy Lesniak | CG Supervisor |
| James Kuroda | Compositing Supervisor |
| Christopher Grutzmacher | Pipeline Technical Director |
| Steve Cady | Senior Animator |
| Chris W. Tucker | Visual Effects Coordinator |
| Anthony Ruey | Visual Effects Coordinator |
| Sarah Yunrong Yu | Visual Effects Coordinator |
| Dane Allan Smith | Visual Effects Producer |
| Reza Amidi | Digital Intermediate Editor |
| Mandy Arnold | Digital Intermediate Editor |
| Adrian J. Sciutto | Visual Effects Coordinator |
| Chrissy Callahan | Visual Effects Coordinator |
| Momo Zhan | Visual Effects Coordinator |
| Brian Crosbie | Visual Effects Production Assistant |
| Evan Fulton | Visual Effects Production Assistant |
| Brad J. Hayes | CG Artist |
| Ben Breckenridge | Stereoscopic Supervisor |
| Marlies Schacherl | Visual Effects Coordinator |
| Natasha Anne Francis | Visual Effects Producer |
| Melissa Franco | Visual Effects Production Assistant |
| Sebastian Butenberg | Animation Supervisor |
| Chris Waegner | CG Supervisor |
| Ross Newton | CG Supervisor |
| Theodore Bialek | CG Supervisor |
| Adam Ghering | Compositing Supervisor |
| Sebastian Kral | Pipeline Technical Director |
| Linda Drake | Visual Effects Editor |
| Hnedel Maximore | CG Artist |
| Brian Howald | Compositing Supervisor |
| Eric D Legare | Pipeline Technical Director |
| Gavin Wright | Pre-Visualization Supervisor |
| Katrissa 'Kat' Peterson | Visual Effects Coordinator |
| Chelsea Kammeyer | Visual Effects Coordinator |
| Pam Hammarlund | Visual Effects Producer |
| Kathleen Latlip | Assistant Editor |
| John Persichetti | Digital Colorist |
| Thomas Cheng | CG Artist |
| Andrew Turner | Visual Effects Coordinator |
| Claudia Butenberg | Visual Effects Coordinator |
| Kai Schadwinkel | CG Artist |
| Brandon Bartlett | CG Supervisor |
| Duncan Rochfort | Visual Effects Editor |
| Steve Love | First Assistant Director |
| Adam Watkins | CG Supervisor |
| Robin Scott Graham | Compositing Supervisor |
| Danielle DiMarco Barto | Visual Effects Coordinator |
| Nancy Lamontagne | Visual Effects Coordinator |
| Morning Star Schott | Digital Intermediate Producer |
| Robert Mead | First Assistant Editor |
| Tong Tran | CG Artist |
| David Alexander Smith | Digital Effects Supervisor |
| Marvin Kim | Modelling Supervisor |
| Michael Langford | Senior Animator |
| Laura M. Meredith | Visual Effects Coordinator |
| Eric Levin-Hatz | Visual Effects Coordinator |
| Robert Drwila | Assistant Editor |
| Steve Bowen | Digital Intermediate Colorist |
| Amy Lu | Senior Animator |
| Genevieve Claire | Visual Effects Coordinator |
| Perry Kain | Visual Effects Coordinator |
| Vincent Truitner | CG Animator |
| Craig Cesareo | Pipeline Technical Director |
| Louise Baker | Pre-Visualization Supervisor |
| Dan Cortez | Visual Effects Coordinator |
| Mingzhe Yang | Visual Effects Coordinator |
| Shanshan Xie | Visual Effects Coordinator |
| Jordan Soles | Visual Effects Producer |
| Giang T. Nguyen | CG Artist |
| Max Tyrie | Lead Animator |
| Ina Marczinczik | Senior Animator |
| Colin de Andrade | Visual Effects Coordinator |
| Phelicia Sperrazzo | Visual Effects Coordinator |
| Jordan Freda | Visual Effects Producer |
| David Schaub | Animation Supervisor |
| Robert Winter | CG Supervisor |
| John Bowers | Compositing Supervisor |
| Shawn Smolensky | Visual Effects Production Assistant |
| Richard Smith | Lead Animator |
| Eddie Chew | Senior Animator |
| Veronica Kablan | Visual Effects Coordinator |
| Davell Lee Libbett | Visual Effects Coordinator |
| Wendy Washbrook | Extras Casting |
| Benjamin Sutor | Online Editor |
| Elizabeth Chodar | Casting Assistant |
| Lauren Cokeley | Extras Casting |
| Andy Henry | Casting Associate |
| Susanne Scheel | Casting Assistant |
| Sara Conte | Extras Casting |
| Ivory Stanton | Ager/Dyer |
| Jennifer Vecchiarello | First Assistant Editor |
| Jerad Marantz | Character Designer |
| Lisa Curtis Saunders | Software Engineer |
| Steve Ditko | Comic Book |
| Stan Lee | Comic Book |
| Gary Archer | Prosthetics |
| Gine Lui | Location Scout |
| Robin Lynn Bonaccorsi | Stunts |
| Vic Armstrong | Stunts |
| Jennifer Caputo | Stunt Double |
| Jill Brown | Stunt Driver |
| Samantha MacIvor | Stunt Double |
| Jennifer Lamb | Stunts |
| Christina Fong | Second Assistant Director |
| Shawnna Thibodeau | Stunts |
| Aja Frary | Stunts |
