 | Ben Stiller als Larry Daley |
 | Amy Adams als Amelia Earhart |
 | Owen Wilson als Jedediah Smith |
 | Hank Azaria als Kahmunrah / The Thinker / Abe Lincoln |
 | Robin Williams als Theodore Roosevelt |
 | Christopher Guest als Ivan the Terrible |
 | Alain Chabat als Napoleon Bonaparte |
 | Steve Coogan als Octavius |
 | Ricky Gervais als Dr. McPhee |
 | Bill Hader als George Armstrong Custer |
 | Jon Bernthal als Al Capone |
 | Patrick Gallagher als Attila the Hun |
 | Jake Cherry als Nicky Daley |
 | Rami Malek als Ahkmenrah |
 | Mizuo Peck als Sacajawea |
 | Kerry van der Griend als Neanderthal #1 |
 | Matthew Harrison als Neanderthal #2 |
 | Rick Dobran als Neanderthal #3 |
 | Randy Lee als Hun #1 |
 | Darryl Quon als Hun #2 |
 | Gerald Wong als Hun #3 |
 | Paul Chih-Ping Cheng als Hun #4 |
 | Jay Baruchel als Sailor Joey Motorola |
 | Mindy Kaling als Docent |
 | Keith Powell als Tuskegee Airman #1 |
 | Craig Robinson als Tuskegee Airman #2 |
 | Samuel Patrick Chu als Teenage Boy #1 |
 | Augustus Oicle als Teenage Boy #2 |
| Kai James als Teenage Boy #3 |
 | Clint Howard als Air and Space Mission Control Tech #1 |
 | Matty Finochio als Air and Space Mission Control Tech #2 |
 | George Foreman als George Foreman |
 | Josh Byer als Capone Gangster #1 |
 | Sophie Levy als Young Girl #1 |
 | Tess Levy als Young Girl #2 |
 | Christina Schild als 'American Gothic' Woman |
 | Robert Thurston als 'American Gothic' Farmer |
 | Alberta Mayne als Kissing Nurse |
 | Clifton MaCabe Murray als Kissing Sailor |
 | Caroll Spinney als Oscar the Grouch (voice) |
| Thomas Morley als Darth Vader |
 | Dan Joffre als Town Car Driver |
| Dave Hospes als Astronaut |
 | Regina Taufen als New York Reporter |
 | Shawn Levy als Infomercial Father |
 | Joe Jonas als Cherub #1 (voice) |
 | Kevin Jonas als Cherub #2 (voice) |
 | Nick Jonas als Cherub #3 (voice) |
 | Eugene Levy als Albert Einstein (voice) |
 | Brad Garrett als Easter Island Head (voice) |
 | Ed Helms als Larry Daley's Assistant (uncredited) |
 | Jonah Hill als Brundon the Security Guard (uncredited) |
 | Thomas Lennon als Wilbur Wright (uncredited) |
 | Crystal the Monkey als Dexter / Able (uncredited) |
| Shawn Levy | Director |
| Shawn Levy | Producer |
| Thomas Lennon | Screenplay |
| Chris Columbus | Producer |
| Michael Barnathan | Producer |
| Aravind Jayaraman | Lighting Artist |
| Guy Bar'ely | Animation |
| Joel Foster | Animation |
| Julie D'Antoni | Visual Effects |
| Sagar Patil | Visual Effects Art Director |
| Mike Marcuzzi | Digital Effects Supervisor |
| Daniel Lindsey | VFX Artist |
| James Bamford | Stunts |
| J.J. Makaro | Stunt Coordinator |
| Helen Jarvis | Supervising Art Director |
| Alan Silvestri | Music |
| John Schwartzman | Director of Photography |
| Krista Bell | Stunt Double |
| Chad Bellamy | Stunts |
| Greg Hanson | Stunt Double |
| Trevor Addie | Stunts |
| Nickolas Baric | Stunts |
| Loyd Bateman | Stunts |
| Bill Anagnos | Utility Stunts |
| Robert Alonzo | Utility Stunts |
| Fraser Aitcheson | Stunts |
| Lloyd Adams | Stunts |
| Donna Isaacson | Casting |
| Marlene Stewart | Costume Design |
| Dan DeLeeuw | Visual Effects Supervisor |
| Ellen Somers | Associate Producer |
| Don Zimmerman | Editor |
| Dean Zimmerman | Editor |
| Claude Paré | Production Design |
| Thomas M. Hammel | Executive Producer |
| Josh McLaglen | Executive Producer |
| Mark Radcliffe | Executive Producer |
| Anne M. Simonet | Unit Production Manager |
| Thomas M. Hammel | Unit Production Manager |
| Josh McLaglen | First Assistant Director |
| Misha Bukowski | Second Assistant Director |
| Eric Hedayat | Production Supervisor |
| Garrett Warren | Stunt Coordinator |
| Gary Capo | Second Unit Director |
| Lin MacDonald | Set Decoration |
| William H. Orr | Special Effects Coordinator |
| Dean Eilertson | Property Master |
| Jason B. Landels | Assistant Property Master |
| Michelle Hendriksen | Assistant Property Master |
| Claudio Palavecino | Assistant Property Master |
| Grant Van Der Slagt | Art Direction |
| Michael Diner | Art Direction |
| Sean Goojha | Art Department Coordinator |
| Lance White | Video Assist Operator |
| Ian Fox | "A" Camera Operator |
| Ian Fox | Second Unit Director of Photography |
| David Crone | "B" Camera Operator |
| David Crone | Steadicam Operator |
| Richard Mosier | First Assistant "A" Camera |
| Patrick Stepien | First Assistant "B" Camera |
| Justin Bergler | Second Assistant Camera |
| Tyler Woeste | Second Assistant Camera |
| Jean-Marc Saldini | Camera Loader |
| Doane Gregory | Still Photographer |
| Rob Young | Production Sound Mixer |
| Kelly Zombor | Boom Operator |
| Darryl Marko | Utility Sound |
| Diane Durant | Script Supervisor |
| Herbert Dwight Raymond IV | First Assistant Editor |
| Jeff Beattie | Visual Effects Editor |
| Terry Wilson | Supervising Music Editor |
| Craig Henighan | Supervising Sound Editor |
| John A. Larsen | Supervising Sound Editor |
| Paul Massey | Sound Re-Recording Mixer |
| David Giammarco | Sound Re-Recording Mixer |
| Laurie Dobbie | Property Buyer |
| Michael A. Billings | Assistant Set Decoration |
| Zoe Jirik | Set Decorating Coordinator |
| Joel Venti | Storyboard Artist |
| Michael Anthony Jackson | Storyboard Artist |
| Marc Baird | Storyboard Artist |
| Mira Caveno | Set Designer |
| Jay Mitchell | Set Designer |
| Bryan Sutton | Set Designer |
| Barbara Harris | ADR Voice Casting |
| Victoria Down | Makeup Department Head |
| Sherry Linder-Gygli | Hair Department Head |
| Andrew W. Davidson | Gaffer |
| Sean Oxenbury | Rigging Gaffer |
| Keith Woods | Rigging Gaffer |
| Shane Dobie | Best Boy Electric |
| Mike Kirilenko | Key Grip |
| David McIntosh | Key Rigging Grip |
| David Cameron | Best Boy Grip |
| Ryan Monro | Dolly Grip |
| Darin Wong | Dolly Grip |
| John Casey | Costume Supervisor |
| Jana MacDonald | Costume Coordinator |
| John Catron | Production Accountant |
| Fawn McDonald | Production Coordinator |
| Rino Pace | Location Manager |
| Jason Collier | Assistant Location Manager |
| Daniel Mansfield | Second Second Assistant Director |
| John Dale | Construction Coordinator |
| Mario Tomas-Niedworok | Paint Coordinator |
| Patricia Johnson | Unit Publicist |
| Rob Vreugde | Transportation Coordinator |
| Craig Henighan | Sound Designer |
| R.J. Kizer | Supervising ADR Editor |
| Laura Graham | ADR Editor |
| Vanessa Lapato | ADR Editor |
| Coll Anderson | Sound Effects Editor |
| John Murray | Foley Supervisor |
| Scott Curtis | Foley Editor |
| Doug Jackson | Foley Editor |
| Charleen Richards-Steeves | ADR Mixer |
| Skip Longfellow | First Assistant Sound Editor |
| Jason Tuttle | Assistant Sound Editor |
| Eric A. Larsen | Assistant Sound Editor |
| Tim Gomillion | Sound Recordist |
| David Lucarelli | ADR Recordist |
| Dan O'Connell | Foley Artist |
| John T. Cucci | Foley Artist |
| James Ashwill | Foley Mixer |
| Jim Passon | Color Timer |
| Eileen Godoy | Digital Intermediate Producer |
| Devon Miller | Digital Intermediate Editor |
| Raymond Chen | Visual Effects Supervisor |
| Bill Tlusty | Visual Effects Producer |
| Audra Neil | Set Decoration Buyer |
| Milan Trenc | Characters |
| Robert Ben Garant | Screenplay |
| Jennifer Mylrea | Utility Stunts |
| Bianca Giancoli | Utility Stunts |
| Kimberly Chiang | Stunts |
| J.J. Perry | Utility Stunts |
| Lauro Chartrand | Stunts |
| Clint Carleton | Stunt Double |
