 | Cedric Hardwicke als Dr. Ludwig Frankenstein / Dr. Henry Frankenstein (ghostly image) |
 | Ralph Bellamy als Erik Ernst |
 | Lionel Atwill als Dr. Bohmer |
 | Bela Lugosi als Ygor |
 | Evelyn Ankers als Elsa Frankenstein |
 | Lon Chaney Jr. als The Frankenstein Monster |
 | Janet Ann Gallow als Cloestine Hussman |
 | Barton Yarborough als Dr. Kettering |
 | Doris Lloyd als Martha the Housekeeper |
 | Leyland Hodgson als Chief Constable |
 | Olaf Hytten als Adolph Hussman |
 | Holmes Herbert als Magistrate |
 | Richard Alexander als Villager at Council Meeting (uncredited) |
 | Lionel Belmore als Councilman (uncredited) |
 | Chet Brandenburg als Villager (uncredited) |
 | Colin Clive als Dr. Henry Frankenstein (flashback) (uncredited) |
 | Harry Cording als Mob Leader (uncredited) |
 | George Eldredge als Constable (uncredited) |
 | Dwight Frye als Villager at Meeting / Grave Robber (flashback) (uncredited) |
 | Lawrence Grant als Mayor (uncredited) |
 | Otto Hoffman als Villager (uncredited) |
 | Brandon Hurst als Hans, Villager at Council Meeting (uncredited) |
 | Michael Mark als Councilman (uncredited) |
| Jimmy Phillips als Boy Playing Indian (uncredited) |
 | William Smith als Boy (uncredited) |
 | Ernie Stanton als Constable (uncredited) |
 | Julius Tannen als Sektal (uncredited) |
 | Harry Tenbrook als Villager at Hearing (uncredited) |
| Glen Walters als Mother of Hungry Children (uncredited) |
 | Janet Warren als Goose-herd (uncredited) |
 | Teddy Infuhr als Boy (uncredited) |
| George Waggner | Producer |
| Erle C. Kenton | Director |
| Scott Darling | Screenplay |
| Russell A. Gausman | Set Decoration |
| Ted J. Kent | Editor |
| Elwood Bredell | Director of Photography |
| Ellis Burman | Makeup Artist |
| Vera West | Costume Design |
| Milton Krasner | Director of Photography |
| Jack Pierce | Makeup Artist |
| Hans J. Salter | Original Music Composer |
| Mary Shelley | Characters |
| Charles Carroll | Sound |
| Bernard B. Brown | Sound Director |
| Eric Taylor | Original Story |
| Charles S. Gould | Assistant Director |
| John P. Fulton | Special Effects |
| Eddie Parker | Stunts |
| Charles Maxwell | Orchestrator |
| Charles Previn | Music |
| Harold H. MacArthur | Art Direction |
| Jack Otterson | Art Direction |
