 | Noomi Rapace als Dr. Elizabeth Shaw |
 | Michael Fassbender als David |
 | Guy Pearce als Peter Weyland |
 | Idris Elba als Janek |
 | Logan Marshall-Green als Charlie Holloway |
 | Charlize Theron als Meredith Vickers |
 | Sean Harris als Fifield |
 | Rafe Spall als Millburn |
 | Emun Elliott als Chance |
 | Benedict Wong als Ravel |
 | Kate Dickie als Ford |
 | Branwell Donaghey als Mercenary #1 |
 | Vladimir "Furdo" Furdik als Mercenary #2 |
 | C.C. Smiff als Mercenary #3 |
 | Shane Steyn als Mercenary #4 |
 | Ian Whyte als Last Engineer |
 | John Lebar als Ghost Engineer |
 | Daniel James als Sacrifice Engineer |
 | Patrick Wilson als Shaw's Father |
 | Lucy Hutchinson als Young Shaw |
 | Giannina Facio als Shaw's Mother |
 | Anil Biltoo als Linguist Teacher |
 | Louisa Staples als Greeting Message Violinist |
 | James Embree als Mechanic #1 |
 | Florian Robin als Mechanic #2 |
 | Matthew Burgess als Mechanic #3 |
 | Eugene O'Hare als Mechanic #4 |
 | Richard Thomson als Archaeological Assistant |
 | Philip McGinley als Archaeological Assistant |
 | Jenny Rainsford als Archaeological Assistant |
 | Rhona Croker als Archaeological Assistant |
| Wambui Wa-Ngatho als Automated Voice (Swahili) (voice) |
| Wannaporn Rienjang als Automated Voice (Thai) (voice) |
| Zed Sevcikova als Automated Voice (Czech) (voice) |
| Sonam Dugdak als Automated Voice (Tibetan) (voice) |
| Reynir Thor Eggertsson als Automated Voice (Icelandic) (voice) |
| Shin-Ichiro Okajima als Automated Voice (Japanese) (voice) |
| Charalambos Dendrinos als Automated Voice (Greek) (voice) |
| Berhane Woldegabriel als Automated Voice (Amharic) (voice) |
| Annie Penn als Ship Computer Voice (voice) |
 | Robin Atkin Downes als Ship Computer Voice (voice) |
| James Currie als Weyland Guest (uncredited) |
 | Dan Dewhirst als Peter Weyland Files (uncredited) |
 | Harry Fowler als William Potter (archive footage) (uncredited) |
| Ian MacNaughton als Hartley (archive footage) (uncredited) |
 | Phill Martin als Elephantine Engineer (uncredited) |
 | Arnold Montey als Elephantine Engineer (uncredited) |
 | Peter O'Toole als Lawrence (archive footage) (uncredited) |
| Matt Rook als Elder Engineer (uncredited) |
| Stuart Saunders als R.S.M. (archive footage) (uncredited) |
| Alex Cameron | Art Direction |
| Janty Yates | Costume Design |
| Arthur Max | Production Design |
| Avy Kaufman | Casting |
| Sonja Klaus | Set Decoration |
| Ridley Scott | Producer |
| Damon Lindelof | Writer |
| Nina Gold | Casting |
| Michael Ellenberg | Executive Producer |
| Walter Hill | Producer |
| Mark Huffam | Executive Producer |
| Jon Spaihts | Writer |
| Pietro Scalia | Editor |
| Damon Lindelof | Executive Producer |
| Ridley Scott | Director |
| Marc Streitenfeld | Original Music Composer |
| Dariusz Wolski | Director of Photography |
| Michael Costigan | Executive Producer |
| Karen Wakefield | Art Direction |
| David Giler | Producer |
| John King | Supervising Art Director |
| Rob Inch | Stunt Coordinator |
| Perry Evans | Gaffer |
| Helen Xenopoulos | Assistant Art Director |
| Mark P. Stoeckinger | Supervising Sound Editor |
| Michelle Eisenreich | Visual Effects Producer |
| Georgiana Sayer | Set Costumer |
| Marc Homes | Art Direction |
| Graham Hall | Camera Operator |
| Daniele Massaccesi | Steadicam Operator |
| Jamie Dixon | Visual Effects Supervisor |
| Gary Spratling | Camera Operator |
| Steve Hart | Location Manager |
| Ron Bartlett | Sound Re-Recording Mixer |
| Bruno de Santa | Set Costumer |
| Catherine Charlton | Dialect Coach |
| Annette Wullems | Visual Effects Producer |
| Tom Whitehead | Assistant Art Director |
| John T. Cucci | Foley |
| Sarah Griggs | Art Department Coordinator |
| Trent Claus | Visual Effects Supervisor |
| Calandra Meredith | Set Costumer |
| Annie Penn | Script Supervisor |
| Anthony Caron-Delion | Art Direction |
| Paul Inglis | Art Direction |
| Victor Ray Ennis | Supervising Sound Editor |
| Dan O'Connell | Foley |
| Martin Hill | Visual Effects Supervisor |
| Claudio Campana | Assistant Art Director |
| Dick Edwards | Visual Effects Supervisor |
| Joseph Bonn | Music Editor |
| Allen Maris | Visual Effects Producer |
| Laura Davison | Construction Coordinator |
| Tom Weaving | Assistant Art Director |
| James Newell | Scenic Artist |
| David Morgan | Camera Operator |
| Kerry Brown | Still Photographer |
| Daniele Massaccesi | Camera Operator |
| Del Spiva | Music Editor |
| Amal Baggar | Script Coordinator |
| Gordon Harmer | Costume Supervisor |
| Peter Devlin | Transportation Coordinator |
| Doug Hemphill | Sound Re-Recording Mixer |
| Peter Dorme | Art Direction |
| Philip Elton | Assistant Art Director |
| Michael Queen | Compositors |
| Kirsty Whalley | Music Editor |
| Nana Fischer | Hair Designer |
| Nicky Knowles | Hairstylist |
| Miranda Marks | Production Coordinator |
| Enzo Angileri | Hair Designer |
| Aisling Nairn | Makeup Artist |
| Peta Dunstall | Hairstylist |
| Jana Carboni | Makeup Artist |
| David Vyle Levy | Conceptual Design |
| Mark 'Rocky' Evans | Electrician |
| Julie Feiner | Dialogue Editor |
| Margit Pfeiffer | Dialogue Editor |
| Michael Hertlein | Dialogue Editor |
| Daniel S. Irwin | Supervising Dialogue Editor |
| Dennis Rogers | Sound Recordist |
| Tim Gomillion | Sound Recordist |
| Charley Henley | Visual Effects Supervisor |
| Trevor Wood | Visual Effects Supervisor |
| Richard Stammers | Visual Effects Supervisor |
| Neville Page | Concept Artist |
| Ronald Shusett | Characters |
| Dan O'Bannon | Characters |
| Paul Butterworth | Visual Effects Designer |
| Carolina Jiménez | Layout |
| Maher Asaad Baker | Visual Effects |
| Archie Campbell-Baldwin | Art Department Assistant |
| H. R. Giger | Art Direction |
| Mike Potter | Stunts |
| Davide Losi | Prosthetics |
| Ferran Domenech | Animation Supervisor |
| Simon Hayes | Production Sound Mixer |
