 | James Arnold Taylor als Leonardo (voice) |
 | Mitchell Whitfield als Donatello / Additional Voices (voice) |
 | Nolan North als Raphael / Nightwatcher (voice) |
 | Mikey Kelley als Michelangelo (voice) |
 | Sarah Michelle Gellar als April O'Neil (voice) |
 | Chris Evans als Casey Jones (voice) |
 | Mako als Splinter (voice) |
 | Patrick Stewart als Winters (voice) |
 | Kevin Smith als Diner Cook (voice) |
 | Laurence Fishburne als Narrator (voice) |
 | Zhang Ziyi als Karai (voice) |
 | John DiMaggio als Colonel Santino (voice) |
 | Paula Mattioli als General Serpiente (voice) |
 | Kevin Michael Richardson als General Aguila / Additional Voices (voice) |
 | Fred Tatasciore als General Gato / Additional Voices (voice) |
 | Dee Bradley Baker als Additional Voices (voice) |
 | Greg Baldwin als Splinter (voice) |
 | Jeff Bennett als Additional Voices (voice) |
 | Jim Cummings als Additional Voices (voice) |
 | Grey DeLisle als Additional Voices (voice) |
 | Chris Edgerly als Additional Voices (voice) |
 | Kim Mai Guest als Additional Voices (voice) |
 | Jennifer Hale als Additional Voices (voice) |
 | Jess Harnell als Additional Voices (voice) |
 | Phil LaMarr als Additional Voices (voice) |
 | Paul Michael Robinson als Additional Voices (voice) |
 | Tara Strong als Additional Voices (voice) |
 | Billy West als Additional Voices (voice) |
 | Kevin Munroe als Diner Patron (voice) (uncredited) |
| Thomas K. Gray | Producer |
| Kevin Munroe | Director |
| Galen Walker | Producer |
| Simon Murton | Production Design |
| John Damien Ryan | Editor |
| Steve Lumley | Director of Photography |
| Kevin Munroe | Writer |
| Kevin Eastman | Characters |
| Peter Laird | Characters |
| Adam Holmes | Layout |
| Earl A. Hibbert | Visual Effects Editor |
| Mona Heftre | Head of Production |
| Barbara Russo | Post Production Producer |
| Shuk Fan Leung | Production Manager |
| Peter Laird | Executive Producer |
| Felix Ip | Co-Producer |
| Kyle Clark | Producer |
| Gary Richardson | Executive Producer |
| Francis Kao | Executive Producer |
| Justine Lam | Production Manager |
| Michael J. Arnold | Line Producer |
| Jojo Chuk | Production Manager |
| William Maurer | Production Manager |
| Kris Zhai | Production Supervisor |
| Paul Wang | Producer |
| Frederick U. Fierst | Executive Producer |
| Tom Gray Thomas Gray | Producer |
| Brad Arensman | Post Production Supervisor |
| Shanna Bassinger | Production Manager |
| Klaus Badelt | Original Music Composer |
