 | Yo Oizumi als Professor Layton (voice) |
 | Maki Horikita als Luke Triton (voice) |
 | Saki Aibu als Emmy Altava (voice) |
 | 水樹奈々 als Janice Quatlane (voice) |
 | Atsuro Watabe als Jean Descole (voice) |
 | 大塚芳忠 als Inspector Clamp Grosky (voice) |
 | 家弓家正 als Oswald Whistler (voice) |
 | 折笠富美子 als Melina Whistler (voice) |
 | 井上喜久子 als Celia Raidley (voice) |
 | 諸星すみれ als Nina (voice) |
 | 飯塚昭三 als Curtis O'Donnell (voice) |
 | 三宅健太 als Marco Brock (voice) |
 | 豊口めぐみ als Amelia Ruth (voice) |
 | 中田譲治 als Frederick Bargland (voice) |
 | LiLiCo als Annie Dretche (voice) |
 | 山寺宏一 als Pierre Starbuck (voice) |
 | 稲葉実 als Don Paolo (voice) |
 | 斎藤志郎 als Inspector Chelmey (voice) |
 | 能登麻美子 als Flora Reinhold (voice) |
 | 納谷六朗 als Dr. Andrew Schrader (voice) |
| 橋本昌和 | Director |
| Tomohito Nishiura | Original Music Composer |
| 徳田夢之介 | Key Animation |
| Nobutoshi Ogura | Key Animation |
| 堀内博之 | Key Animation |
| Hiroshi Shimizu | Key Animation |
| 岡村玲子 | Key Animation |
| 杉光登 | Key Animation |
| Mitsutoshi Kubo | Key Animation |
| 海谷敏久 | Key Animation |
| 杉光登 | Animation Director |
| 堀内博之 | Animation Director |
| Norihiro Matsubara | Animation Director |
| Sayuri Ichiishi | Animation Director |
| Shinichi Yoshino | Animation Director |
| 鍋田香代子 | Animation Director |
| 高橋英樹 | Animation Director |
| 川面恒介 | Animation Director |
| 川面恒介 | Key Animation |
| 鍋田香代子 | Key Animation |
| 菅沼芙実彦 | Key Animation |
| 早川麻美 | Key Animation |
| 藤井康雄 | Key Animation |
| 小島明日香 | Key Animation |
| Kento Toya | Key Animation |
| 大東百合恵 | Key Animation |
| 大導寺美穂 | Key Animation |
| Katsumi Ikeda | Key Animation |
| Takao Maki | Key Animation |
| Tsuyoshi Yoshioka | Key Animation |
| 石井明治 | Key Animation |
| Kenji Irie | Key Animation |
| Miyuki Ueda | Key Animation |
| Akira Kojima | Key Animation |
| 伊勢奈央子 | Key Animation |
| 中西彩 | Key Animation |
| Takashi Tsuda | Key Animation |
| Atsushi Ogata | Key Animation |
| Yasue Ohno | Key Animation |
| 小山知洋 | Key Animation |
| Tomoki Ogawa | Key Animation |
| Junpei Fukuchi | Key Animation |
| Hosato Shima | Key Animation |
| 鈴木美咲 | Key Animation |
| 牧野博美 | Key Animation |
| Yasukazu Shoji | Key Animation |
| Koji Fukasawa | Key Animation |
| Hidetoshi Owashi | Key Animation |
| Tadaaki Miyata | Key Animation |
| 吉田優子 | Key Animation |
| 宮岡真弓 | Key Animation |
| 夏住愛子 | Key Animation |
| Yuko Iwaoka | Key Animation |
| 黒石崇裕 | Key Animation |
| 久保雅一 | Executive Producer |
| Kazuya Hamana | Executive Producer |
| Naoya Fujimaki | Co-Executive Producer |
| 阿部秀司 | Co-Executive Producer |
| 橋本昌和 | Storyboard Artist |
| 今石洋之 | Storyboard Artist |
| Joji Shimura | Storyboard Artist |
| Junji Nishimura | Storyboard Artist |
| 増井壮一 | Storyboard Artist |
| Naoto Yamatani | Sound Effects |
| 西佐知子 | Sound Effects |
| 緒方康恭 | Sound Effects |
| Tomokazu Mii | Sound Effects |
| 長谷川卓也 | Sound Effects |
| Akiko Muto | Sound Effects |
| 倉橋静男 | Sound Effects |
| 三間雅文 | Sound Director |
| 竹田悠介 | Art Direction |
| Toshio Henmi | Editor |
| Riko Shinohara | Art Direction |
| 杉光登 | Character Designer |
| 杉光登 | Supervising Animation Director |
| Aya Matsui | Screenplay |
| Toshiaki Okuno | Producer |
| 堀川憲司 | Producer |
| Arimasa Okada | Producer |
| 山内章弘 | Producer |
| Tsuneyoshi Saito | Main Title Theme Composer |
| Hiroshi Aso | Co-Producer |
| Toshihiro Kitta | Co-Producer |
| Koji Goromaru | Co-Producer |
| 菊池宣広 | Co-Producer |
| Madoka Hosoya | Co-Producer |
| 佐藤孝一 | Key Animation |
