 | Boris Karloff als The Monster |
 | Ernest Thesiger als Doctor Pretorius |
 | Colin Clive als Henry Frankenstein |
 | O. P. Heggie als Hermit |
 | Una O'Connor als Minnie |
 | Valerie Hobson als Elizabeth |
 | Dwight Frye als Karl |
 | Elsa Lanchester als Mary Wollstonecraft Shelley / The Monster's Bride |
 | Ted Billings als Ludwig |
 | E. E. Clive als Burgomaster |
 | Gavin Gordon als Lord Byron |
 | Douglas Walton als Percy Bysshe Shelley |
 | Reginald Barlow als Hans |
 | Lucien Prival als Butler |
 | Mary Gordon als Hans' Wife |
 | Anne Darling als Shepherdess |
 | Billy Barty als Baby (uncredited) |
 | Robert Adair als Hunter in Woods (uncredited) |
| Norman Ainsley als Archbishop (uncredited) |
| Frank Benson als Villager (uncredited) |
 | Maurice Black als Gypsy (uncredited) |
 | Walter Brennan als Peasant (uncredited) |
| Mae Bruce als Villager (uncredited) |
| A.S. Byron als King Homunculus (uncredited) |
 | John Carradine als Lost Hunter at Hermit's Cottage (uncredited) |
 | D'Arcy Corrigan als Procession Leader (uncredited) |
 | Grace Cunard als Villager (uncredited) |
| J. Gunnis Davis als Uncle Glutz (uncredited) |
| Marie DeForrest als Ballerina (uncredited) |
 | Elspeth Dudgeon als Gypsy's Mother (uncredited) |
 | Helen Jerome Eddy als Gypsy's Wife (uncredited) |
| Neil Fitzgerald als Rudy (uncredited) |
 | Brenda Fowler als Mother (uncredited) |
 | John George als Villager (uncredited) |
 | Helen Gibson als Villager (uncredited) |
 | Marilyn Harris als Girl (uncredited) |
 | Carmencita Johnson als Friend of Murdered Girl (uncredited) |
 | Rollo Lloyd als Neighbor (uncredited) |
 | Murdock MacQuarrie als Sympathetic Villager (uncredited) |
| Josephine McKim als Little Mermaid (uncredited) |
 | Torben Meyer als Victim in flashback (uncredited) |
| Edwin Mordant als Coroner (uncredited) |
| Charles Murphy als Guard (uncredited) |
| Joseph North als Servant (uncredited) |
 | Helen Parrish als Communion Girl (uncredited) |
 | Edward Peil Sr. als Villager (uncredited) |
 | Tempe Pigott als Auntie Glutz (uncredited) |
| Sarah Schwartz als Marta (uncredited) |
| Peter Shaw als Devil (uncredited) |
| Mary Stewart als Neighbor (uncredited) |
 | Frank Terry als Lost Hunter at Hermit's Cottage (uncredited) |
| Anders Van Haden als Villager (uncredited) |
 | Dorothy Vernon als Maid (uncredited) |
| Lucio Villiegas als Priest (uncredited) |
 | Joan Woodbury als Queen Homunculus (uncredited) |
| Carl Laemmle Jr. | Producer |
| Ted J. Kent | Editor |
| Franz Waxman | Original Music Composer |
| James Whale | Director |
| John J. Mescall | Director of Photography |
| Vera West | Costume Design |
| Charles D. Hall | Art Direction |
| William Hurlbut | Adaptation |
| William Hurlbut | Screenplay |
| Maurice Pivar | Supervising Film Editor |
| John L. Balderston | Adaptation |
| Jack Pierce | Makeup Artist |
| Peter Shaw | Stand In |
| Fred Stoll | Grip |
| John P. Fulton | Special Effects |
| C. Bakaleinikoff | Orchestrator |
| Robert Florey | Story |
| Irma Kusely | Hairstylist |
| Otto Lederer | Assistant Makeup Artist |
| Gilbert Kurland | Sound Supervisor |
| William Hedgcock | Sound Engineer |
| Josef Berne | Adaptation |
| Lawrence G. Blochman | Adaptation |
| Philip MacDonald | Adaptation |
| Tom Reed | Additional Writing |
| R.C. Sherriff | Adaptation |
| Edmund Pearson | Screenplay |
| Morton Covan | Adaptation |
| Irma Kusely | Hair Setup |
| Otto Lederer | Makeup Trainee |
| Fred Frank | Assistant Director |
| Harry Mancke | Assistant Director |
| Joseph A. McDonough | Assistant Director |
| David S. Horsley | Special Effects Assistant |
| Ken Strickfaden | Special Effects Technician |
| John P. Fulton | Visual Effects Camera |
| Cleo E. Baker | Visual Effects |
| Russell Lawson | Visual Effects |
| Jack Cosgrove | Visual Effects |
| David S. Horsley | Visual Effects |
| William Dodds | Assistant Camera |
| Alan Jones | Camera Operator |
| Larry Aicholtz | Scoring Mixer |
| Abe Meyer | Music Coordinator |
| Clifford Vaughan | Orchestrator |
| Oliver Wallace | Musician |
| Flo Brummel | Script Consultant |
| George DeNormand | Stand In |
| Monte Montague | Stand In |
| Edwin Wetzel | Sound Effects Editor |
| Mary Shelley | Original Story |
