 | Owen Wilson als Reggie (voice) |
 | Woody Harrelson als Jake (voice) |
 | Amy Poehler als Jenny (voice) |
 | George Takei als S.T.E.V.E. (voice) |
 | Colm Meaney als Myles Standish (voice) |
 | Keith David als Chief Broadbeak (Voice) |
 | Dan Fogler als Governor William Bradford (voice) |
 | Lesley Nicol als Female Pilgrim (voice) |
 | Jimmy Hayward als President of the United States/Ranger/Leatherbeak/Hazmats (voice) |
 | Kaitlyn Maher als President's Daughter (voice) |
 | Carlos Alazraqui als Amos (voice) |
 | Josh Lawson als Gus (Voice) |
 | Dwight Howard als Cold Turkey (Voice) |
 | Danny Carey als Danny (voice) |
 | Carlos Ponce als Narrator/Alejandro (voice) |
 | Robert Beltran als Chief Massasoit (voice) |
 | Scott Mosier als Pizza Dude (voice) |
| Scott Mosier | Screenplay |
| Jimmy Hayward | Screenplay |
| Jimmy Hayward | Director |
| Jimmy Almeida | Animation |
| Tyler DeLisle | Lighting Artist |
| Andy Hass | Animation |
| Sue Houston Safianoff | Animation |
| Dixie Pizani | Animation |
| Travis Tohill | Animation |
| Jay Jackson | Animation |
| Scott Mosier | Producer |
| David I. Stern | Story |
| John J. Strauss | Story |
| Aron Warner | Executive Producer |
| John J. Strauss | Executive Producer |
| David I. Stern | Executive Producer |
| Dominic Lewis | Original Music Composer |
| Chris Cartagena | Editor |
| Kevin R. Adams | Production Design |
| Rich McKain | Supervising Animator |
| David Esneault | Digital Supervisor |
| Scott Peterson | Digital Supervisor |
| Jeff Biancalana | Head of Story |
| Kerry Rock | Casting Director |
| Paul Downs | Animation |
| Phil Langone | Storyboard Artist |
