 | Milla Jovovich als Alice |
 | Oded Fehr als Carlos Olivera |
 | Ali Larter als Claire Redfield |
 | Iain Glen als Dr. Isaacs |
 | Ashanti als Betty |
 | Christopher Egan als Mikey |
 | Spencer Locke als K-Mart |
 | Matthew Marsden als Slater |
 | Linden Ashby als Chaser |
 | Jason O'Mara als Albert Wesker |
 | Mike Epps als L.J. |
 | Joe Hursley als Otto |
 | James Tumminia als Lab Tech |
 | Madeline Carroll als White Queen |
 | Valorie Hubbard als Ma |
 | Carrie Eklund als Camp Survivor (uncredited) |
 | Brian Steele als Rancid / Tyrant |
 | John Eric Bentley als Umbrella Tech |
 | Kirk B.R. Woller als Scientist |
 | Rick Cramer als Ice Hockey / Corridor Guard |
 | Peter O'Meara als British Envoy |
 | William Abadie als French Envoy |
 | Ramón Franco als Runty |
 | Shane Woodson als Piggy |
 | Geoff Meed als Pock Mark |
 | Rusty Joiner als Eddie |
 | Connor McCoy als Small Boy |
 | Carolina Jiménez als Mirror Woman |
 | Gary A. Hecker als Tyrant Vocal (voice) |
 | Martin Crewes als Chad Kaplan (uncredited) |
 | Richard Epcar als Additional Voices (voice) (uncredited) |
 | Stefen Hayes als Yuri Loginova (uncredited) |
 | Gary Hudson als Umbrella Captain (uncredited) |
 | Ryan McCluskey als Mr. Grey (uncredited) |
 | Carsten Norgaard als Zombie Steve (uncredited) |
 | César Ámigo Aguilar als Foreign Envoy (uncredited) |
| Russell Mulcahy | Director |
| Paul W. S. Anderson | Producer |
| Jeremy Bolt | Producer |
| Bernd Eichinger | Producer |
| Samuel Hadida | Producer |
| Victor Hadida | Executive Producer |
| Robert Kulzer | Producer |
| Héctor López Lechuga | Line Producer |
| Martin Moszkowicz | Executive Producer |
| Kelly Van Horn | Executive Producer |
| Charlie Clouser | Original Music Composer |
| David Johnson | Director of Photography |
| Niven Howie | Editor |
| Scot Boland | Casting |
| Victoria Burrows | Casting |
| Eugenio Caballero | Production Design |
| Marco Niro | Art Direction |
| Barbara Enriquez | Set Decoration |
| Joseph A. Porro | Costume Design |
| David Forrest | Makeup Department Head |
| Sylvia Fernández | Assistant Hairstylist |
| Tena Parker | Assistant Hairstylist |
| Carol Brown | Assistant Makeup Artist |
| Elvia Felix Herrera | Assistant Makeup Artist |
| Laura Hill | Contact Lens Technician |
| Bunny Parker | Hair Department Head |
| Isabel Amezcua | Hairdresser |
| Estrella Lorrabaquio | Hairdresser |
| Lourdes Flores | Key Makeup Artist |
| Humberto Escamilla | Makeup Artist |
| Marco A. Garcia | Makeup Artist |
| Richard Redlefsen | Makeup Effects |
| Jorge Siller | Makeup Effects |
| Gabriel Tapia | Art Department Coordinator |
| Carlos Benassini | Assistant Art Director |
| Alejandro García | Assistant Set Decoration |
| Patricia Cuevas | Assistant Set Decoration |
| Sandra Flores | Assistant Set Decoration |
| Miguel Pineda | Construction Buyer |
| Héctor Romero | Construction Manager |
| Dereck Hecker | Property Master |
| Gustavo Castellanos | Property Master |
| Guillermo Granados | Props |
| Carlos Torres | Props |
| Jonathan Gesinski | Storyboard Artist |
| Mauricio Waldo | Swing |
| Reyes Santamaría V. | Best Boy Electric |
| Victor Correa | Best Boy Electric |
| Salvador 'Apache' Serrano | Best Boy Grip |
| Cesar Islas Manjarrez | Camera Loader |
| Gerardo Ruffinelli | Camera Loader |
| Eduardo Flores Torres | Camera Loader |
| Pablo Reyes | Camera Operator |
| Tim Ross | Camera Operator |
| Luis David Sansans | Camera Operator |
| Antonio Huerta | Camera Technician |
| Ernesto Serrano | Dolly Grip |
| Geovani Rodriguez | Dolly Grip |
| Manuel García | Electrician |
| Néstor Martínez | Electrician |
| Luis Velazquez | Electrician |
| Miguel Lara | Electrician |
| Saul Dominguez | Electrician |
| Roman Covarrubias | Electrician |
| Rob Wood | First Assistant "A" Camera |
| Israel Páez | First Assistant "A" Camera |
| Sergio García Terrazas | First Assistant "B" Camera |
| Jose Gutierrez | First Assistant "B" Camera |
| Jorge Zúñiga | First Assistant "C" Camera |
| Fernando Peralta | First Assistant "C" Camera |
| Benito Aguilar | Gaffer |
| Juan Carlos Lazo | Gaffer |
| Pablo Castillo | Grip |
| Juan Cruz | Grip |
| Adolfo Rodríguez | Grip |
| César Camacho | Grip |
| Salvador Orendain | Grip |
| Miguel A. Pacheco | Grip |
| Erik Gonzales | Grip |
| Andrés Medina | Key Grip |
| Peter Chrimes | Key Grip |
| Antonio Vargas | Key Grip |
| Juan Pablo Ortigosa M. | Second Assistant "A" Camera |
| Fernando Gutierrez | Second Assistant "A" Camera |
| Fernando Díaz | Second Assistant "B" Camera |
| Fernando Sanchez | Second Assistant "B" Camera |
| Katie Matheson | Second Assistant "C" Camera |
| Igor Meglic | Second Unit Director of Photography |
| Luis David Sansans | Steadicam Operator |
| Norbert Kaluza | Steadicam Operator |
| Rolf Konow | Still Photographer |
| Ana Nuñez | Assistant Costume Designer |
| Arturo Mayne | Costume Assistant |
| Marco Villavicencio | Costume Assistant |
| María de Jesús Luna | Costume Assistant |
| Joseph A. Porro | Costume Designer |
| Adolfo Ramírez | Costume Supervisor |
| Leticia Pichardo | Costumer |
| Francisco Stoll | Costumer |
| Rocio Ceja | Costumer |
| Pedro Raúl Flores | Tailor |
| James Simcik | ADR Editor |
| Bill Devine | ADR Mixer |
| Jeff Gomillion | ADR Mixer |
| Dean St. John | ADR Mixer |
| Terry Garcia | Assistant Sound Editor |
| Jesus Alvarez | Boom Operator |
| José Manuel Aguilar | Boom Operator |
| Nestor Cruz Sanchez | Cableman |
| Joan Rowe | Foley Artist |
| Sean Rowe | Foley Artist |
| Ryan Maguire | Foley Mixer |
| Luciano Larobina | Sound Mixer |
| Alberto Ramirez | Sound Mixer |
| Chris Jenkins | Sound Re-Recording Mixer |
| Frank A. Montaño | Sound Re-Recording Mixer |
| Miguel Rivera | Supervising ADR Editor |
| Miguel Rivera | Supervising Dialogue Editor |
| Derek Pippert | Foley |
| Eric A. Norris | Supervising Sound Editor |
| Scott A. Hecker | Supervising Sound Editor |
| Dan Carnegie | Animation |
| Hubert Chan | Animation |
| Peter Dydo | Animation |
| Jason Edwardh | Animation |
| Elliot Mallon | Animation |
| Matt Ralph | Animation |
| Gavin Soares | Animation |
| Jim Su | Animation |
| Mike Mulock | Animation |
| Robert Bloom | Compositor |
| Ian Britton | Compositor |
| Megumi Kanazawa | Compositor |
| Hojin Park | Compositor |
| Ruben Salazar | Compositor |
| John Watson | Compositor |
| Lisa-Marie Marrelli | Compositor |
| Barb Benoit | Digital Compositor |
| Jason Chan | Digital Compositor |
| Ovidiu Cinazan | Digital Compositor |
| Anand Dorairaj | Digital Compositor |
| Omar Gudjonsson | Digital Compositor |
| Annu Gulati | Digital Compositor |
| Avi Salem | Digital Compositor |
| Carolyn Shelby | Digital Compositor |
| Tamara Stone | Digital Compositor |
| David Thompson | Digital Compositor |
| Armando Velazquez | Digital Compositor |
| John-John Traoré | Digital Compositor |
| Kenny Lam | Digital Compositor |
| Michael Becki | Digital Compositor |
| Tim M. Townsend | Digital Compositor |
| Neil Williamson | Digital Effects Supervisor |
| Aaron Weintraub | Digital Effects Supervisor |
| Tom Turnbull | Digital Effects Supervisor |
| Andrew Nguyen | Lead Animator |
| Mandy Au | Lighting Artist |
| Dominic Remane | Lighting Artist |
| Mark Stepanek | Lighting Artist |
| Cyril Frederick Chu | Lighting Artist |
| Sarah Barber | Production Assistant |
| Matt Glover | Production Assistant |
| Aisha Malik | Production Assistant |
| Sandeep Bachhe | Sequence Lead |
| Palak Krishna Murthy | Sequence Lead |
| Jesh Murthy | Sequence Lead |
| Aaron Barclay | Sequence Lead |
| Rob Del Ciancio | Sequence Lead |
| Daniel Mizuguchi | Sequence Lead |
| Wojciech Zielinski | Sequence Lead |
| Rubina Cokar | Visual Effects Coordinator |
| Luke Groves | Visual Effects Coordinator |
| Victoria Holt | Visual Effects Coordinator |
| Mark Herman | Visual Effects Editor |
| Fiona Campbell Westgate | Visual Effects Producer |
| Dennis Berardi | Visual Effects Supervisor |
| Evan Jacobs | Visual Effects Supervisor |
| James Bladon | Music Editor |
| Tod Feuerman | Additional Editor |
| Ryan Folsey | Additional Editor |
| Richard Glazerman | Assistant Editor |
| Chris Mistorni | Assistant Editor |
| David Raymond | Assistant Editor |
| Ben Howdeshell | Assistant Editor |
| Tristan M. Brighty | Assistant Editor |
| Michael Mintz | Digital Intermediate Colorist |
| Joseph Alaniz | Digital Intermediate Producer |
| Kevin LaRosa | Aerial Coordinator |
| Jesús Almontes | Armorer |
| Charles Taylor | Armorer |
| Karla Bukantz | Assistant Production Coordinator |
| Carlos Larios | Assistant Production Coordinator |
| Mauricio Ballesteros | Craft Service |
| Howard Baral | Post Production Accountant |
| Yolanda Chavarria | Post Production Accountant |
| Dominique Donovan | Post Production Accountant |
| Carla Raygoza | Production Coordinator |
| Patrick Zorer | Production Executive |
| Liz Brandenburg | Production Secretary |
| Sue Field | Script Supervisor |
| Israel Pasco | Script Supervisor |
| Bruno Hernandez | Security |
| Norberto Nava | Security |
| Mario Olivares | Security |
| Jose Luis Palomino | Set Medic |
| Sarahi Marin | Set Production Assistant |
| Edgar Gallaga | Set Production Assistant |
| Manuel Castro | Set Production Assistant |
| David Oliver | Set Production Assistant |
| Nancy A. Flint | Studio Teacher |
| Christopher C. Jones | Title Graphics |
| Andrea Gamboa | Travel Coordinator |
| Yhara Mejia | Travel Coordinator |
| Kym Langlie | Unit Publicist |
| Javier Gunther | Picture Car Coordinator |
| Xavier Rodriguez Quiriarte | Transportation Captain |
| Balo Bucio | Stunt Coordinator |
| Rick Forsayeth | Stunt Coordinator |
| Layton Morrison | Stunt Coordinator |
| Steven McMichael | Stunt Coordinator |
| Joanne Leach | Stunt Double |
| Patrick Tatopoulos | Concept Artist |
| José Castellanos | Lighting Technician |
| Sharon Simms | Stunt Double |
| Michiko Nishiwaki | Stunt Double |
| Krista Bell | Utility Stunts |
| Chrissy Weathersby Ball | Stunt Double |
| Priscilla Solorzano | Stunt Double |
| Miriam Meza | Stunt Double |
| Nadia Hernandez | Utility Stunts |
| Martha Alicia | Utility Stunts |
| Paul W. S. Anderson | Writer |
| O.J. Knighten | Animal Wrangler |
