 | Cate Blanchett als Elizabeth I, Queen of England |
 | Clive Owen als Sir Walter Raleigh |
 | Geoffrey Rush als Sir Francis Walsingham |
 | Laurence Fox als Sir Christopher Hatton |
 | Tom Hollander als Amyas Paulet |
 | Abbie Cornish als Elizabeth Throckmorton |
 | Rhys Ifans als Robert Reston |
 | Jordi Mollà als King Philip II of Spain |
 | Samantha Morton als Mary, Queen of Scots |
 | Eddie Redmayne als Anthony Babington |
 | Adrian Scarborough als Calley |
 | Adam Godley als William Walsingham |
 | Christian Brassington als Archduke Charles |
 | Robert Cambrinus als Count Georg von Helfenstein |
 | David Threlfall als Dr. John Dee |
 | Vidal Sancho als Spanish Minister |
 | Kelly Hunter als Ursula Walsingham |
 | John Shrapnel als Lord Howard |
 | Sam Spruell als Torturer |
 | David Sterne als Cellarman |
 | David Robb als Admiral Sir William Winter |
 | Jonathan Bailey als Courtier |
| Steve Lately als Walsingham's Servant |
 | Kate Fleetwood als Woman with Baby |
| Aimee King als Infanta Isabel of Spain |
 | Susan Lynch als Annette |
| Kristin Coulter Smith als Mary Walsingham |
 | Hayley Burroughs als Queen Elizabeth's Waiting Lady #1 |
| Kirsty McKay als Queen Elizabeth's Waiting Lady #2 |
| Lucia Ruck Keene als Queen Elizabeth's Waiting Lady #3 |
| Lucienne Venisse-Back als Queen Elizabeth's Waiting Lady #4 |
| Elise McCave als Laundry Woman |
| Penelope McGhie als Margaret |
| Coral Beed als First Court Lady |
 | Rosalind Halstead als Second Court Lady |
 | Steven Loton als Manteo |
| Martin Baron als Wanchese |
 | David Armand als Walsingham's Agent |
 | Steven Robertson als Sir Francis Throckmorton |
| Jeremy Barker als Ramsey |
 | George Innes als Burton |
| Kirstin Smith als Mary Walsingham |
 | Tim Preece als Old Throckmorton |
| Benjamin May als Dance Master |
| Glenn Doherty als Royal Servant |
 | Chris Brailsford als Dean of Peterborough |
 | Dave Legeno als Executioner |
 | Antony Carrick als Spanish Archbishop |
 | John Atterbury als Marriage Priest |
| Alex Giannini als First Spanish Officer |
 | Joe Ferrara als Second Spanish Officer |
| Alexander Barnes als Courtier |
| Charles Bruce als Courtier |
| Jeremy Cracknell als Courtier |
| Benedict Green als Courtier |
| Adam Smith als Courtier |
| Simon Stratton als Courtier |
| Crispin Swayne als Courtier |
| Kitty Fox als Mary Stuart's Lady in Waiting |
| Kate Lindesay als Mary Stuart's Lady in Waiting |
| Katherine Templar als Mary Stuart's Lady in Waiting |
| Morne Botes als Courtier (uncredited) |
| Finn Morrell als Young Boy (uncredited) |
| Shane Nolan als Tyger Salior (uncredited) |
| Shekhar Kapur | Director |
| Remi Adefarasin | Director of Photography |
| William Nicholson | Screenplay |
| Michael Hirst | Screenplay |
| Jill Bilcock | Editor |
| Andrew Haddock | Editor |
| Fiona Weir | Casting |
| Richard Roberts | Set Decoration |
| Alexandra Byrne | Costume Design |
| Craig Armstrong | Original Music Composer |
| A.R. Rahman | Original Music Composer |
| David Allday | Art Direction |
| Christian Huband | Art Direction |
| Jason Knox-Johnston | Art Direction |
| Phil Sims | Art Direction |
| Andy Thomson | Art Direction |
| Morag Ross | Hairstylist |
| Dorka Nieradzik | Hairstylist |
| Marion McCormack | Hairstylist |
| Susan Howard | Makeup Artist |
| Joe Hopker | Makeup Artist |
| Uxue Laguardia | Makeup Artist |
| Mark Mostyn | Production Manager |
| Anna Skrein | Art Department Coordinator |
| James Gemmill | Scenic Artist |
| Rohan Harris | Scenic Artist |
| Steve Mitchell | Scenic Artist |
| Helen Xenopoulos | Assistant Art Director |
| Ian Whiteford | Greensman |
| Duncan McDevitt | Sculptor |
| Stephen McClure | Sculptor |
| Alexandra Harwood | Sculptor |
| Steve Deahl | Sculptor |
| Mark Auguste | Supervising Sound Editor |
| Tim Cavagin | Sound Re-Recording Mixer |
| Steve Single | Sound Re-Recording Mixer |
| Ian Ward | CG Supervisor |
| Michelle Corney | Visual Effects Producer |
| Victoria Mowlam | Visual Effects Producer |
| Aled Robinson | Visual Effects Editor |
| John Lockwood | Visual Effects Supervisor |
| Richard Stammers | Visual Effects Supervisor |
| Steve Street | Visual Effects Supervisor |
| Greg Powell | Stunt Coordinator |
| Simon Finney | Camera Operator |
| Mark Milsome | Camera Operator |
| Ben Wilson | Camera Operator |
| Roger Tooley | Steadicam Operator |
| Alf Tramontin | Steadicam Operator |
| Laurie Sparham | Still Photographer |
| David Worley | Additional Camera |
| Suzi Turnbull | Costume Supervisor |
| Martin Chitty | Set Costumer |
| Peter K. Christopher | Set Costumer |
| Fiona McCann | Set Costumer |
| Maurizio Torti | Set Costumer |
| Billy Browne | First Assistant Editor |
| Tony Lewis | Music Editor |
| Jane Gibson | Choreographer |
| Angela Wharton | Script Supervisor |
| Penny Dyer | Dialect Coach |
| Nick Daubeny | Location Manager |
| Alex Gladstone | Location Manager |
| Manus Home | Location Manager |
| Stacy Mann | Unit Publicist |
| Frank Walsh | Supervising Art Director |
| Sam Auguste | Dialogue Editor |
| Tim Hands | ADR Editor |
| David Balfour | Property Master |
| Alan Stewart | Second Unit Director of Photography |
| Mario Vaccaro | Foley Artist |
| Andrew Neil | Foley Editor |
| Robert Edwards | ADR Recordist |
| Michele Tandy | Finance |
| Kirsten Lane | Music Consultant |
| Andy Smart | Stunts |
| Gordon Seed | Stunts |
| Dominic Preece | Stunts |
| Peter Pedrero | Stunts |
| Ray Nicholas | Stunts |
| Daniel Naprous | Stunts |
| Mark Mottram | Stunts |
| Peter Miles | Stunts |
| Marc Mailley | Stunts |
| Paul Kennington | Stunts |
| John Kearney | Stunts |
| Rowley Irlam | Stunts |
| Rob Inch | Stunts |
| Gary Hoptrough | Stunts |
| Paul Herbert | Stunts |
| James Grogan | Stunt Double |
| Andy Godbold | Stunts |
| Aldonio Danny Frietas | Stunts |
| Pete Ford | Stunts |
| Dave Fisher | Stunts |
| Bradley Farmer | Stunts |
| Rick English | Stunts |
| Ben Dimmock | Stunts |
| George Cottle | Stunts |
| Abbi Collins | Stunt Double |
| Nick Chopping | Stunts |
| David Anders | Stunts |
| Guy Hendrix Dyas | Production Design |
