 | Wesley Snipes als Eric Brooks / Blade |
 | Stephen Dorff als Deacon Frost |
 | Kris Kristofferson als Abraham Whistler |
 | N'Bushe Wright als Dr. Karen Jenson |
 | Donal Logue als Quinn |
 | Udo Kier als Gitano Dragonetti |
 | Arly Jover als Mercury |
 | Traci Lords als Racquel |
 | Kevin Patrick Walls als Officer Krieger |
 | Tim Guinee als Dr. Curtis Webb |
 | Sanaa Lathan als Vanessa Brooks |
 | Eric Edwards als Pearl |
 | Donna Wong als Nurse |
 | Carmen Thomas als Senior Resident |
 | Shannon Lee als Resident |
 | Kenny Johnson als Heatseeking Dennis |
 | Clint Curtis als Creepy Morgue Guy |
 | Judson Scott als Pallantine |
 | Sidney S. Liufau als Japanese Doorman |
 | Keith Leon Williams als Kam |
 | Andray Johnson als Paramedic |
 | Stephen R. Peluso als Paramedic |
| Marcus Aurelius als Pragmatic Policeman |
 | John Enos III als Blood Club Bouncer |
 | Eboni 'Chrystal' Adams als Martial Arts Kid |
 | Lyle Conway als Lyle Conway |
 | Freeman White als Menacing Stud |
 | D.V. DeVincentis als Vampire Underling |
 | Marcus Salgado als Frost's Goon |
 | Esau McKnight als Frost's Goon |
 | Erl Van Douglas als Von Esper |
 | Matt Schulze als Crease |
 | Lennox Brown als Pleading Goon |
| Yvette Ocampo als Party Girl |
 | Irena Stepić als Slavic Vampire Lord |
 | Jenya Lano als Russian Woman |
 | Levan Uchaneishvili als Russian Vampire |
| Richard 'Dr.' Baily als Cardboard cut-out in Subway (uncredited) |
| Nikki DiSanto als Vampire Victim (uncredited) |
 | Ryan Glorioso als Blood Bath Vampire (uncredited) |
 | Jeff Imada als Henchman (uncredited) |
| Elliott James als Blood Club (uncredited) |
 | Stephen Norrington als Vampire (uncredited) |
 | Gerald Okamura als Vampire (uncredited) |
 | Frankie Ray als Vampire Lord (uncredited) |
| Carrie Seeley als Woman in Elevator (uncredited) |
| Beth Theriac als Woman in Elevator (uncredited) |
 | Ted King als Vampire at rave (uncredited) |
 | Steven Ho als Frost's Goon |
 | Will Leong als Henchman (uncredited) |
 | Al Goto als Henchman (uncredited) |
| Stephen Norrington | Director |
| David S. Goyer | Writer |
| Robert Engelman | Producer |
| Peter Frankfurt | Producer |
| Wesley Snipes | Producer |
| Avi Arad | Executive Producer |
| Joseph Calamari | Executive Producer |
| Lynn Harris | Executive Producer |
| Stan Lee | Executive Producer |
| Michael De Luca | Executive Producer |
| Mark Isham | Original Music Composer |
| Theo van de Sande | Director of Photography |
| Paul Rubell | Editor |
| Rachel Abroms | Casting |
| Jory Weitz | Casting |
| Sanja Milković Hays | Costume Design |
| Jon Divens | Co-Producer |
| Andrew J. Horne | Co-Producer |
| Kirk M. Petruccelli | Production Design |
| Barry Chusid | Art Direction |
| Greg J. Grande | Set Decoration |
| Michael Germain | Makeup Department Head |
| Garet Reilly | Costume Supervisor |
| Thell Reed | Armorer |
| Renate Leuschner | Wigmaker |
| Cyndi Reece-Thorne | Makeup Artist |
| Kim Santantonio | Hairstylist |
| Melissa C. Ho | Art Department Coordinator |
| Anthony Lattanzio | Construction Coordinator |
| Chad S. Frey | Set Designer |
| A. Todd Holland | Set Designer |
| Anthony F. Jimenez | Leadman |
| Laurah Grijalva | Lead Painter |
| Charli Palazzo | Lead Painter |
| Harry Cohen | Supervising Sound Effects Editor |
| Greg Hedgepath | Supervising Sound Effects Editor |
| Harry Cohen | Sound Designer |
| Ken Teaney | Sound Re-Recording Mixer |
| Sean Rowe | Foley |
| Lou Carlucci | Special Effects Coordinator |
| Jennifer Bergman | Visual Effects Producer |
| Matthew Justice | Visual Effects Producer |
| Zeke Morales | Visual Effects Editor |
| Chris DeAngelis | Visual Effects Editor |
| William Plant | Visual Effects Supervisor |
| Kevin O'Neill | Visual Effects Supervisor |
| Mark Kolpack | Visual Effects Supervisor |
| Richard E. Hollander | Visual Effects Supervisor |
| Chuck Comisky | Visual Effects Supervisor |
| Moshe Levin | Camera Operator |
| Randy Nolen | Steadicam Operator |
| Bruce W. Talamon | Still Photographer |
| David Katz | Video Assist Operator |
| Jono Kouzouyan | Gaffer |
| Daniele Colajacomo | Animation Supervisor |
| Thomas A. Carlson | Music Editor |
| Carlton Kaller | Music Editor |
| Paul Rabjohns | Music Editor |
| Craig Fehrman | Transportation Coordinator |
| J. Marc Strachan | Location Manager |
| Guy Adan | Unit Publicist |
| Jennifer Teves | Production Supervisor |
| Jeff Ward | Stunt Coordinator |
| Paul Curtis | ADR Editor |
| John C. Stuver | ADR Editor |
| Robert J. Lattanzio | Painter |
| Miles Teves | Sculptor |
| Patrick Janicke | Production Illustrator |
| A. Anthony Cappello | First Assistant Camera |
| Bill Cousins | Grip |
| Joy Zapata | Hair Department Head |
| Judy Crown | Key Hair Stylist |
| Marina Babitchenko | Seamstress |
| Gregory B. Peña | Set Costumer |
| Gus Olafsson | Set Dressing Artist |
| Kenneth Brooks | Carpenter |
| Lisa Y. Turner | Chef |
| Amin Chande | Craft Service |
| Antoine Durr | Digital Effects Supervisor |
| Anthony Ardito | Driver |
| Sara Romilly | Post Production Supervisor |
| Thomas S. Dickson | Projection |
| Brian Berkel | Propmaker |
| David O. Krupnick | Set Medic |
| Rodney Barnes | Set Production Assistant |
| Lennox Brown | Stand In |
| Henry Kingi Jr. | Stunts |
| Bob Froehlig | Systems Administrators & Support |
| Tommy Hooper | Technical Supervisor |
| Walter McClain | Transportation Captain |
| Mato | Color Timer |
| Gigi Rivkin | Editorial Production Assistant |
| Catherine Chase | First Assistant Editor |
| Greg Kittelson | Best Boy Electric |
| R. Bruce Prochal | Electrician |
| Kate Choi | Lighting Technician |
| Jody Levin | Executive In Charge Of Post Production |
| Carla Fry | Executive In Charge Of Production |
| Jonathan Davidson | Production Accountant |
| Marlene Hart | Production Coordinator |
| Danny Rubio | Production Manager |
| Carey Ann Strelecki | Researcher |
| Randall L. Johnson | Boom Operator |
| Dana Sano | Music Supervisor |
| Ken Kugler | Orchestrator |
| Ken King | Production Sound Mixer |
| Michael Kamper | Sound Effects Editor |
| Everett Burrell | 3D Supervisor |
| Dennis Bennett | Digital Compositors |
| Lisa Ann Glass | Visual Effects Coordinator |
| Miles Teves | Conceptual Design |
| Larry Odien | Animatronic and Prosthetic Effects |
| Wesley Snipes | Martial Arts Choreographer |
| Stuart Mintz | Lighting Supervisor |
| Gary Nolin | Line Producer |
| Roee Sharon | Unit Production Manager |
| Paul Prokop | Production Controller |
| Erica Froker | Camera Loader |
| Michael Neumann | First Assistant Director |
| Al Eisenmann | Assistant Property Master |
| Tony Sepian | Dolly Grip |
| Robert Gray | Key Grip |
| Sandy Kenyon | Key Costumer |
| Brian Wade | Makeup Effects Designer |
| Freddy Negrete | Tattoo Designer |
| Perrin Cutting | Digital Effects Producer |
| Ray McCort | Pilot |
| Rebecca Strickland | Second Assistant Director |
| Barbara Harris | ADR Voice Casting |
| Anthea Strangis | Assistant Production Coordinator |
| Kimberly Auslander | Casting Assistant |
| John Chandler | Foley Editor |
| Ryan Ferro | Utility Sound |
| Lance Armstrong | 3D Artist |
| Andy Clement | 3D Animator |
| Chow Emrich | Lead Animator |
| Paul A. Edwards | Camera Operator |
| Nicole Rubio | Script Supervisor |
| John C. Stuver | Dialogue Editor |
| Paul Curtis | Dialogue Editor |
| Steven Melton | Property Master |
| Lee Orloff | Sound Mixer |
| John Owens | Electrician |
| Kate Samhat | Costume Assistant |
| Irena Stepić | Costumer |
| Derek Johansen | Second Second Assistant Director |
| Jason Brubaker | Storyboard Artist |
| Jonathan Bobbitt | Swing |
| Eric Thompson | ADR Mixer |
| Colin Rogers | ADR Recordist |
| Pembrooke Andrews | Assistant Dialogue Editor |
| Joan Rowe | Foley Artist |
| Shawn Kennelly | Foley Recordist |
| Richard Terry Tjelmeland | Special Effects Technician |
| Allen Maris | Visual Effects Production Assistant |
| Clayton J. Barber | Stunt Double |
| Jennifer Bell | Assistant Camera |
| Michael Stuart Clark | Best Boy Grip |
| Garrett Benson | Second Assistant Camera |
| Mike Sowa | Digital Color Timer |
| Brandon Smith | Post Production Coordinator |
| Stephen Sobisky | Project Manager |
| Rocky Brooks | Assistant Location Manager |
| Patrick Noonan | Location Production Assistant |
| Bob Bowen | Music Coordinator |
| Kelley Rogers | Art Department Production Assistant |
| Mario Estarella | Assistant Chef |
| Linda Drake | Effects Supervisor |
| Marcy Brown | Extras Casting Assistant |
| Carolyn Noonan | First Assistant Accountant |
| Ervin Wayne Spowehn | Generator Operator |
| Richard Crompton | Key Rigging Grip |
| Steve Gilbert | Rigging Grip |
| Dino Juico | Production Assistant |
| Leon Dudevoir | Production Executive |
| Orian Williams | Second Assistant Production Coordinator |
| Marv Wolfman | Characters |
| Gene Colan | Characters |
| Steven Ho | Stunts |
| Christopher Romano | Sequence Supervisor |
| Gary Archer | Prosthetics |
| David Leitch | Stunts |
| Jeannie Epper | Stunts |
| Anita Hart | Stunts |
| Francine Morris | Stunts |
| Rosine 'Ace' Hatem | Stunts |
| Jeff Imada | Stunts |
| Jennifer Watson-Johnston | Stunts |
| Joyce McNeal | Stunts |
| Diana R. Lupo | Stunts |
| Jeff Brockton | Stunt Double |
| Mark Chadwick | Stunts |
| Kiante Elam | Stunts |
| Eric Chambers | Stunts |
| Mark De Alessandro | Stunt Double |
| Ousaun Elam | Stunt Double |
| Thomas DuPont | Stunts |
| Keith Campbell | Stunts |
| Simone Boisseree | Stunts |
| Danielle Burgio | Stunts |
| Damian Achilles | Stunts |
| Rochelle Ashana | Stunts |
| Kenny Endoso | Stunts |
| Al Goto | Stunts |
| Marjean Holden | Stunts |
| Clay Donahue Fontenot | Stunt Double |
| Hollis Hill | Stunts |
| Marguerite Happy | Stunts |
| Steven Ito | Stunts |
| Henry Kingi Jr. | Stunt Coordinator |
| Henry Kingi | Stunts |
| Diana Lee Inosanto | Stunts |
| Maria R. Kelly | Stunt Double |
| Khristian Lupo | Stunts |
| John C. Meier | Stunts |
| Randolph LeRoi | Stunts |
| Jimmy Nickerson | Stunts |
| Susie Park | Stunts |
| Eddie Perez | Stunts |
| Jane Oshita | Stunts |
| J.J. Perry | Stunts |
| Simon Rhee | Stunts |
| Darrin Prescott | Stunts |
| Kerry Rossall | Stunts |
| Gilbert Rosales | Stunts |
| Erik Rondell | Stunts |
| Jimmy N. Roberts | Stunts |
| Felipe Savahge | Stunts |
| Christina Ritzi | Stunt Double |
| Steven Ritzi | Stunts |
| Danny Wong | Stunts |
| April Weeden | Stunt Double |
| Scott Wilder | Stunts |
| David Wald | Stunts |
| Tim Trella | Stunts |
| Gerald Okamura | Stunts |
| Kim Robert Koscki | Stunts |
| Danny Wynands | Stunts |
| Michiko Nishiwaki | Stunts |
| Brian Smrz | Stunts |
