 | Kyla Pratt als Maya Dolittle |
 | Peter Coyote als President Sterling |
 | Malcolm Stewart als Chief Dorian |
 | Niall Matter als Cole Fletcher |
 | Elise Gatien als Courtney Sterling |
 | Karen Holness als Lisa Dolittle |
 | Christine Chatelain als Selma |
 | Kwesi Ameyaw als Prince Tharoor |
 | Stephanie Belding als Doris Park-Weaver |
 | Christopher Gaze als Academic #1 |
 | Andrew McIlroy als Academic #2 |
 | Kirsten Kilburn als Co-Ed |
 | Adam Thomas als Frat Boy |
 | Jason Griffith als Guard |
 | Alex Kliner als Janitor |
 | Jennifer Coolidge als Daisy (voice) |
 | Richard Kind als Groundhog (voice) |
 | Phil Proctor als Monkey (voice) |
 | Ben Diskin als Anteater (voice) |
 | Greg Ellis als Wallaby (voice) |
 | Nolan North als Parrot (voice) |
 | Diana Yanez als Chinchilla (voice) |
 | Eliza Bayne als Various Talking Animals (voice) (uncredited) |
 | Keith Dallas als Director (uncredited) |
 | Norm Macdonald als Lucky (voice) (uncredited) |
 | Steve Oatway als Mailman (uncredited) |
| Kathleen Laccinole | Co-Writer |
| Matt Lieberman | Writer |
| Craig Shapiro | Director |
| Hugh Lofting | Story |
| Norma Hill-Patton | Makeup Designer |
| Jennifer Page | Casting |
| Don MacDonald | Music |
| Marian Koprada | Third Assistant Director |
| Matt Lieberman | Story |
| Kirsten Franson | Art Direction |
| Ron Stannett | Cinematography |
| Victoria Söderholm | Set Decoration |
| Petra Demas | Assistant Editor |
| Craig Forrest | Unit Production Manager |
| Eugene Baldovino | Post Production Assistant |
| Brian Manis | Executive Producer |
| Duane Dickinson | Stunt Coordinator |
| Emanuela Daus | Makeup Artist |
| Alexia S. Droz | Second Assistant Director |
| Ken Lee | Boom Operator |
| Dan Carr | Location Manager |
| Michael Trent | Editor |
| Corinne Clark | Casting |
| Jason L. Wood | Casting |
| Ali Salamati | Third Assistant Director |
| Connie Dolphin | Co-Producer |
| Ingrid Kenning | Script Supervisor |
| David Markowitz | First Assistant Director |
| Ken Davies | Property Master |
| Brentan Harron | Production Design |
| Lorraine Carson | Costume Design |
| Dawn Knight | Post Production Supervisor |
| William Butler | Production Sound Mixer |
| John Davis | Producer |
